A5012066208- Adipokines, Inflammation, and Metabolic Diseases
- Adipose Tissue and Metabolism
- Metabolism, Diabetes, and Cancer
- FOXO transcription factor regulation
- Cardiomyopathy and Myosin Studies
- Bioinformatics and Genomic Networks
- Cardiovascular Disease and Adiposity
- Cardiovascular Effects of Exercise
- Muscle Physiology and Disorders
- Fatty Acid Research and Health
- Nutrition and Health in Aging
- Cardiac Valve Diseases and Treatments
- Immune cells in cancer
- Microbial Metabolic Engineering and Bioproduction
- RNA Research and Splicing
- Gene Regulatory Network Analysis
- Atherosclerosis and Cardiovascular Diseases
- Immune Cell Function and Interaction
- Cardiovascular Function and Risk Factors
- Caveolin-1 and cellular processes
- Protein Kinase Regulation and GTPase Signaling
- PI3K/AKT/mTOR signaling in cancer
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Cancer, Lipids, and Metabolism
- Genetics, Aging, and Longevity in Model Organisms
University of Gothenburg
2018-2025
Sahlgrenska University Hospital
2022-2025
Wallenberg Wood Science Center
2022
Linköping University
2013-2018
Obesity is associated with extensive expansion and remodeling of the adipose tissue architecture, including its microenvironment extracellular matrix (ECM). Although obesity has been reported to induce fibrosis, composition ECM under healthy physiological conditions remained underexplored debated. Here, we used a combination three established techniques (picrosirius red staining, colorimetric hydroxyproline assay, sensitive gene expression measurements) evaluate status in metabolically lean...
The immune checkpoint B7-H3 (CD276) is a member of the B7 family that has been studied in tumor microenvironment and immunotherapy, but its potential role metabolism remains largely unknown. Here, we show highly expressed mouse human adipose tissue at steady state, with highest levels adipocyte progenitor cells. rapidly down-regulated upon initiation differentiation. Combined RNA sequencing metabolic studies reveal stimulates glycolytic mitochondrial activity progenitors. Loss progenitors...
BACKGROUND: Muscle proteins of the obscurin protein family play important roles in sarcomere organization and sarcoplasmic reticulum T-tubule architecture function. However, their precise molecular functions redundancies between members as well involvement cardiac diseases remain to be fully understood. METHODS: To investigate functional Obsc (obscurin) its close homolog Obsl1 (obscurin-like 1) heart, we generated analyzed knockout mice for , Obsc/Obsl1 double knockouts. RESULTS: We show...
Dysregulated chronic inflammation plays a crucial role in the pathophysiology of atherosclerosis and may be result impaired resolution. Thus, restoring levels specialized pro-resolving mediators (SPMs) to promote resolution has been proposed as therapeutic strategy for patients with atherosclerosis, addition standard clinical care. Herein, we evaluated effects SPM lipids, lipoxin A4 (LXA4) B4 (LXB4), on neutrophils isolated from compared healthy controls. Patients displayed altered...
Type 2 diabetes is characterized by insulin resistance in the expanding adipose tissue of obesity. The manifests human adipocytes as system-wide impairment signalling. An exception regulation transcription factor FOXO1 (forkhead box protein O1), which phosphorylated downstream mTORC2 (mammalian/mechanistic target rapamycin complex with raptor) and therefore not exhibiting impaired response to insulin. However, abundance, activity, reduced half from patients diabetes. To elucidate effect we...
The search for biomarkers associated with obesity-related diseases is ongoing, but it not clear whether plasma and serum can be used interchangeably in this process. Here we high-throughput screening to analyze 358 proteins 76 lipids, selected because of their relevance obesity-associated diseases, from age- sex-matched lean obese humans. Most the proteins/lipids had similar concentrations serum, a subset showed significant differences. Notably, key marker cardiovascular disease PAI-1...
The insulin receptor substrate-1 (IRS1) is phosphorylated on serine 307 (human sequence, corresponding to murine 302) in response as part of a feedback loop that controls IRS1 phosphorylation tyrosine residues by the receptor. This turn directly affects downstream signaling and human adipocytes implicated pathogenesis resistance type 2 diabetes. inhibited rapamycin, specific inhibitor mammalian target rapamycin (mTOR) complex with raptor (mTORC1). mTORC1-downstream p70 ribosomal protein S6...
Adipose tissue inflammation drives obesity-related cardiometabolic diseases. Enhancing endogenous resolution mechanisms through administration of lipoxin A4, a specialized pro-resolving lipid mediator, was shown to reduce adipose and subsequently protects against obesity-induced systemic disease in mice. Here, we demonstrate that lipoxins 3D-cultured human adipocytes explants from obese patients. Approximately 50% patients responded particularly well by reducing inflammatory cytokines...
The ubiquitously expressed IQ motif-containing GTPase activating protein-1 (IQGAP1) is a scaffolding protein implicated in an array of cellular functions, particular by binding to cytoskeletal elements and signaling proteins. A role IQGAP1 adipocytes has not been reported. We therefore investigated the interactome primary human adipocytes. Immunoprecipitation quantitative mass spectrometry identified caveolae caveolae-associated proteins as major interactors alongside confirmed...
As our ability to measure the complexity of intracellular networks has evolved, it become increasingly clear that we need new methods for data analysis: involving mathematical modeling. Nevertheless, is still uncontroversial publish and interpret experimental results without a model-based proof reasoning correct. In present study, argue this attitude probably needs change in future. We illustrate modeling by considering common technique using dominant-negative constructs. More specifically,...
Abstract Muscle proteins of the obscurin protein family play important roles in sarcomere organization, sarcoplasmic reticulum (SR) and T-tubule architecture function. However, their precise molecular functions redundancies between members as well involvement cardiac diseases remain to be fully understood. To investigate functional its close homologue obscurin-like 1 (Obsl1) heart, we generated analyzed knockout mice for obscurin, Obsl1, obscurin/Obsl1 double-knockouts (dKO). We show that...
Study Objective Obesity is a predisposing factor for cardiometabolic and kidney disease. Inflammation has been identified as an underlying risk factor. However, studies report conflicting results on the impact of short‐ long‐term weight loss obesity‐related inflammation. Here we investigate levels inflammation in lean, metabolically healthy obese (MHO) unhealthy (MUO) patients, loss, using multiplex arrays bioinformatics analysis. Methods Obese patients scheduled to undergo gastric bypass...
Study Objective Obesity‐related co‐morbidities pose a serious health care system burden. However, 30% of obese subjects do not present with cardiometabolic complications. Here we perform comprehensive lipidome and proteome analyses to identify inflammatory fingerprints that characterize metabolically healthy (MHO) unhealthy (MUO) phenotypes. Methods Obese patients (BMI 35–50) donated plasma before undergoing bariatric surgery (clinicaltrial.gov NCT02322073). Patients were classified as MHO...