A5074664830- HIV Research and Treatment
- HIV/AIDS drug development and treatment
- Cancer-related molecular mechanisms research
- HIV/AIDS Research and Interventions
- RNA Research and Splicing
- HIV-related health complications and treatments
- RNA modifications and cancer
- CAR-T cell therapy research
- Hepatitis C virus research
- RNA Interference and Gene Delivery
- Hormonal Regulation and Hypertension
- Electrolyte and hormonal disorders
- Nanowire Synthesis and Applications
- interferon and immune responses
Ghent University Hospital
2016-2023
Ghent University
2017-2019
University College London
2017
VIB-UGent Center for Inflammation Research
2017
The Royal Free Hospital
2017
Studying the effects of HIV infection on host transcriptome has typically focused protein-coding genes. However, recent advances in field RNA sequencing revealed that long non-coding RNAs (lncRNAs) add an extensive additional layer to cell's molecular network. Here, we performed profiling throughout a primary vitro investigate lncRNA expression at different replication cycle processes (reverse transcription, integration and particle production). Subsequently, guilt-by-association,...
The modification of CD4+ T cells with exogenous nucleic acids or proteins is a critical step in several research and therapeutic applications, such as HIV studies cancer immunotherapies. However, efficient cell transfections are not always easily achieved when working these primary hard-to-transfect cells. While the typically performed by viral transduction electroporation, their use associated safety issues cytotoxicity. Vapor nanobubble (VNB) photoporation sensitizing gold nanoparticles...
HIV-1 DNA quantification serves as an important reservoir biomarker in HIV cure trials. However, the high genetic diversity of represented by different subtypes may bring inaccuracy quantifying and a sensitive validated assay covering diverse is lacking. Therefore, we cross-validated total assays described literature using three-step comparative analysis. First, bioinformatics tool was developed in-house to perform silico evaluation 67 assays. Secondly, these selected were vitro panel and,...
Abstract Background Validated biomarkers to evaluate HIV-1 cure strategies are currently lacking, therefore requiring analytical treatment interruption (ATI) in study participants. Little is known about the safety of ATI and its long-term impact on patient health. Objectives was assessed potential predicting viral rebound were evaluated. Methods PBMCs, plasma CSF collected from 11 HIV-1-positive individuals at four different timepoints during (NCT02641756). Total integrated DNA,...
BackgroundA wide range of host restriction factors (RF) become upregulated upon HIV-1 infection to suppress viral infectivity and may aid viremic control in vivo. This cross-sectional study evaluated RFs dependency HIV infected individuals with progressive or non-progressive infection, as well early late treated cohorts that exhibit different viro-immunological profiles due differences timing treatment-initiation.MethodsThe expression profile IFIT1, MX1, APOBEC3G, SAMHD1, BST2 (encoding...
Lately, the interest in long non-coding RNAs (lncRNAs) as potential drug targets and predictive markers context of HIV-1 has peaked, but their vivo expression regulation remains largely unexplored. Therefore, present study examined lncRNA patterns during a clinical antiretroviral treatment interruption (ATI) trial. Peripheral blood mononuclear cells were isolated from ten patients at four timepoints: prior to ATI, 7-15 days after stop, viral rebound 3 months post therapy re-initiation. RNA...