A5049466934- Neonatal Respiratory Health Research
- Nursing Roles and Practices
- Tendon Structure and Treatment
- Child Abuse and Related Trauma
- Foot and Ankle Surgery
- Orthopedic Surgery and Rehabilitation
- Innovations in Medical Education
- Child Abuse and Trauma
- Musculoskeletal Disorders and Rehabilitation
- Cardiac, Anesthesia and Surgical Outcomes
- Shoulder Injury and Treatment
- Neuroscience of respiration and sleep
- Corneal Surgery and Treatments
- Hospital Admissions and Outcomes
- Cardiovascular Issues in Pregnancy
- Spondyloarthritis Studies and Treatments
- Traumatic Brain Injury and Neurovascular Disturbances
- Infant Nutrition and Health
- Cellular transport and secretion
- Corneal surgery and disorders
- Restraint-Related Deaths
- Immune Response and Inflammation
- Cardiac Arrest and Resuscitation
- Health Sciences Research and Education
- Electrolyte and hormonal disorders
University of Pittsburgh
2016-2024
Allegheny Health Network
2021
University of Pittsburgh Medical Center
2021
Pulmonary Associates
2019
Mercy Medical Center
2019
Saint Louis University
2017
James A. Haley Veterans' Hospital
2017
Magee-Womens Hospital
2017
Pennsylvania State University
2017
Jefferson University Hospitals
2017
Increased mortality from infection in patients with pulmonary alveolar proteinosis occurs association high levels of autoantibodies against granulocyte-macrophage colony-stimulating factor (GM-CSF). We tested the hypothesis that neutrophil functions are impaired and GM-CSF cause dysfunction.We studied 12 subjects proteinosis, 61 healthy control subjects, either cystic fibrosis or end-stage liver disease. also GM-CSF-/- mice wild-type mice. evaluated basal functions, after priming by to...
Granulocyte/macrophage colony-stimulating factor (GM-CSF) autoantibodies (GMAb) are strongly associated with idiopathic pulmonary alveolar proteinosis (PAP) and believed to be important in its pathogenesis. However, levels of GMAb do not correlate disease severity also present at low healthy individuals.Our primary objective was determine whether human would reproduce PAP primates. A secondary the concentration resulting loss GM-CSF signaling vivo (i.e., critical threshold).Nonhuman primates...
Polyuria is a frequent symptom of hyperparathyroidism.Impairment renal concentrating ability, often out proportion to other signs insufficiency, has been noted in patients with hyperparathyroidism (1-3) as well and animals hypercalcemia due causes (4-6).In many hyperpara- thyroidism, extensive deposits calcium the parenchyma, secondary fibrosis, superim- posed pyelonephritis vascular disease have combined destroy considerable amounts substance.The mechanism reported de- crease ability such...
Methane-utilizing bacteria (methanotrophs) are important in both environmental and biotechnological applications, due to their ability convert methane multicarbon compounds. However, systems-level studies of metabolism have not been carried out methanotrophs. In this work we integrated genomic transcriptomic information provide an overview central metabolic pathways for utilization Methylosinus trichosporium OB3b, a model alphaproteobacterial methanotroph. Particulate monooxygenase,...
Acute hypercalcemia was produced by infusing calcium gluconate intravenously in dogs undergoing mannitol diuresis. The fraction of filtered sodium which reabsorbed decreased, association with a fall glomerular filtration rate. Slight to moderate decreases T c H 2 O were observed, but urine did not become hypotonic plasma.
Surfactant protein B (SP-B) is detected in the airways as a sulfhydryl-dependent dimer (M(r) approximately 16,000). To test hypothesis that formation of homodimers critical for SP-B function, cysteine residue reported to be involved dimerization was mutated serine (Cys(248) --> Ser) and targeted distal respiratory epithelium transgenic mice. Transgenic lines which demonstrated appropriate processing, sorting, secretion human monomer were crossed with +/- mice achieve expression absence...
Purpose To assess residents' perceptions of the impact resident work hour restrictions on patient care, education, and job satisfaction. Method Four focus groups internal medicine residents at Barnes-Jewish Hospital Washington University School Medicine were conducted during February March 2004. Twenty-six housestaff from first three years residency participated; all volunteers. Transcripts analyzed for major themes. Results Both interns supported limitations enjoyed benefits working fewer...
Ankle arthritis is a debilitating condition that causes severe functional impairment. While arthrodesis has been the gold standard of surgical treatment for this condition, significant improvements in total ankle arthroplasty have made it viable alternative. The purpose study was to look at midterm follow-up Agility ankle.A retrospective review prospectively collected data conducted on 127 consecutive ankles implanted between 2002 and 2009. Charts were reviewed collect patient demographics....
Desquamative interstitial pneumonia (DIP) is a rare, smoking-related, diffuse parenchymal lung disease characterized by marked accumulation of alveolar macrophages (AMs) and emphysema, without extensive fibrosis or neutrophilic inflammation. Because smoking increases expression pulmonary GM-CSF (granulocyte/macrophage–colony stimulating factor) stimulates proliferation activation AMs, we hypothesized that chronic exposure mice to increased may recapitulate DIP. Wild-type (WT) were subjected...
Allergen challenge in the lung of humans and animals is associated with surfactant dysfunction, but mechanism this effect has not been established. By using a murine model asthma we now report allergen-induced airway inflammation on expression transgenes regulated by human protein (hSP)-C promoter. The hSP-C 3.7-kilobase pair promoter was used to direct eotaxin, an eosinophil-selective chemokine, into lungs several transgenic lines. As expected, mice expressed increased amounts eotaxin mRNA...
Background: CT scans are often used in the evaluation of blunt trauma patients. Many negative. Clinical predictors positive abdominal would be beneficial patient care. Methods: A prospective study 213 patients at a Level I center presenting with who underwent scan. Indications for scan were analyzed statistically, using univariate and multivariate models. Results: Univariate χ2 tests showed abnormal pelvis x-ray (p = 0.0002) an intubated 0.03) When subjected to logistic regression, these two...
✓ Neoplastic angioendotheliosis is a rare disorder characterized by intravascular neoplastic proliferation of endothelial cells within vessels all caliber in the meninges and neuropil. Ischemic infarcts brain spinal cord result from occlusion lumina or fibrin thrombi. Transition reactive to endothelium can be identified many vessels. Steroid therapy beneficial reduction severity symptoms, but cannot alter course disease. Therapeutic intervention must undertaken promptly after diagnosis...
Pediatric condition falsification may masquerade as chronic and serious digestive disease, including intestinal pseudo-obstruction. The purpose of this study was to define clinical criteria discriminate between these two conditions. We compared medical records 8 pediatric victims those 14 children with Clinical features suggesting in toddlers presenting severe complaints included (a) daily abdominal pain, (b) illness involving three or more organ systems, (c) an accelerating disease...
The 79-amino acid, mature SP-B peptide contains three intramolecular disulfide bonds shared by all saposin-like proteins. This study tested the hypothesis that bond formed between cysteine residues 35 and 46 (residues 235 246 of proprotein) is essential for proper function SP-B. To test role this bridge in vivo, a construct was generated which human proprotein were mutated to serine cloned under control 3.7-kilobase hSP-C promoter (hSP-BC235S/C246S). In two transgenic mouse lines, expression...
To achieve durable recognition as a promising animal experiment-abandoning tool in ophthalmology, vitro engineered tissue equivalents of the human cornea should exhibit proper morphogenesis. Regarding this issue, we were seeking for natural cell microenvironment fulfilling minimum requirements to allow corneal keratinocytes develop balanced epithelial morphology with regular spatial appearance homeostatic biomarkers. Hence, established cocultures 3D cell-based collagen scaffolds comprising...