A5065572897- Advanced biosensing and bioanalysis techniques
- Biosensors and Analytical Detection
- Mass Spectrometry Techniques and Applications
- Advanced Photocatalysis Techniques
- Gas Sensing Nanomaterials and Sensors
- Hemoglobin structure and function
- Catalytic Processes in Materials Science
- MicroRNA in disease regulation
- RNA Interference and Gene Delivery
- Electrochemical Analysis and Applications
- Protein Interaction Studies and Fluorescence Analysis
- Analytical Chemistry and Sensors
- Per- and polyfluoroalkyl substances research
- Gold and Silver Nanoparticles Synthesis and Applications
- Parkinson's Disease Mechanisms and Treatments
- Analytical chemistry methods development
- HIV/AIDS drug development and treatment
- Electrochemical sensors and biosensors
- RNA and protein synthesis mechanisms
- Monoclonal and Polyclonal Antibodies Research
- thermodynamics and calorimetric analyses
- Molecular Junctions and Nanostructures
- Biochemical and Structural Characterization
- Cellular transport and secretion
- DNA and Nucleic Acid Chemistry
University of Antwerp
2020-2024
In this manuscript, we compare different analytical methodologies to validate or disprove the binding capabilities of aptamer sequences. This was prompted by lack a universally accepted and robust quality control protocol for characterization performances coupled with observation independent yet inconsistent data sets in literature. As an example, chose three aptamers reported affinity nanomolar range ampicillin, β-lactam antibiotic, used as biorecognition elements several detection...
Abstract Perfluorooctanoic acid (PFOA) is a toxic compound that absorbed and distributed throughout the body by noncovalent binding to serum proteins such as human albumin (hSA). Though interaction between PFOA hSA has been already assessed using various analytical techniques, high resolution detailed analysis of mode still lacking. We report here crystal structure in complex with medium‐chain saturated fatty (FA). A total eight distinct sites, four occupied PFOAs FAs, have identified. In...
Mutations in the Parkinson's disease (PD)-associated protein leucine-rich repeat kinase 2 (LRRK2) commonly lead to a reduction of GTPase activity and increase activity. Therefore, strategies for drug development have mainly been focusing on design LRRK2 inhibitors. We recently showed that central RocCOR domains (Roc: Ras complex proteins; COR: C-terminal Roc) bacterial homolog cycle between dimeric monomeric form concomitant with GTP binding hydrolysis. PD-associated mutations can slow down...
Artemisinin (ART) is a vital medicinal compound that used alone or as part of combination therapy against malaria. ART thought to function by attaching heme covalently and alkylating range proteins. Using biophysical methods, we demonstrate bound three-way junction duplex containing DNA molecules. Binding first shown for the cocaine-binding aptamer extensively studied using this molecule. Isothermal titration calorimetry methods show binding both entropically enthalpically driven at...
Photoelectrochemical detection of nucleic acid-based cancer biomarkers offers opportunities for highly sensitive, selective, and fast quantitative using low-cost measurement instruments. In order to establish itself as a standard method identifying quantifying acids, we have developed multiplexing strategy LED technology photoelectrochemical in 96 samples simultaneously. A dedicated setup based on the 96-well plate configuration with custom-made array was developed. Subsequently,...
The haem group is a promising redox probe for the design of albumin-based voltammetric sensors. Among endogenous ligands carried by human serum albumin (hSA), characterised reversible behaviour and its binding kinetics strongly depend on hSA's conformation, which, in turn, depends presence other ligands. In this work, potential applicability haem, especially hemin, as was first tested proof-of-concept study using perfluorooctanoic acid (PFOA) model analyte. PFOA known to bind hSA occupying...
Native mass spectrometry allows the screening of possible protein bioreceptors for perfluorinated alkyl substances.
MicroRNAs (miRNAs) are small oligonucleotides (18-25 bases), biologically relevant for epigenetic regulation of key processes, particularly in association with cancer. Research effort has therefore been directed towards the monitoring and detection miRNAs to progress (early) cancer diagnoses. Traditional strategies expensive, a lengthy time-to-result. In this study we develop an oligonucleotide-based assay using electrochemistry specific, selective sensitive circulating miRNA (miR-141)...