A5038270737- Electrospun Nanofibers in Biomedical Applications
- Bone Tissue Engineering Materials
- 3D Printing in Biomedical Research
- Tissue Engineering and Regenerative Medicine
- Osteoarthritis Treatment and Mechanisms
- Silk-based biomaterials and applications
- Nanoplatforms for cancer theranostics
- Cellular Mechanics and Interactions
- Nanoparticle-Based Drug Delivery
- Mesenchymal stem cell research
- RNA Interference and Gene Delivery
- Collagen: Extraction and Characterization
- Graphene and Nanomaterials Applications
- Advanced biosensing and bioanalysis techniques
- Advanced Photocatalysis Techniques
- DNA and Nucleic Acid Chemistry
- Advanced Nanomaterials in Catalysis
- biodegradable polymer synthesis and properties
- Periodontal Regeneration and Treatments
- Gold and Silver Nanoparticles Synthesis and Applications
- Extracellular vesicles in disease
- Cancer Cells and Metastasis
- RNA and protein synthesis mechanisms
- Hydrogels: synthesis, properties, applications
- TiO2 Photocatalysis and Solar Cells
National Institute for Materials Science
2016-2025
National Alliance on Mental Illness
2021
Ibaraki University
2019
University of Tsukuba
2015-2017
Weatherford College
2010
Hitachi General Hospital
2009
RIKEN
2007
Nara Institute of Science and Technology
2001-2003
Tokushima University
1999-2001
Kyoto University
1996-2001
Gold nanoparticles (AuNPs) have been extensively explored for biomedical applications due to their advantages of facile synthesis and surface functionalization. Previous studies suggested that AuNPs can induce differentiation stem cells into osteoblasts. However, how the size shape affect response has not elucidated. In this work, a series bovine serum albumin (BSA)-coated Au nanospheres, nanostars nanorods with different diameters 40, 70 110 nm were synthesized effects on osteogenic human...
Gelatin hydrogels can mimic the microenvironments of natural tissues and encapsulate cells homogeneously, which makes them attractive for cartilage tissue engineering. Both mechanical biochemical properties affect phenotype chondrocytes. However, influence each property on chondrocyte is unclear due to difficulty in separating roles these properties. In this study, we aimed study hydrogel stiffness while excluding role factors, such as adhesion site density hydrogels. By altering degree...
There is increasing evidence that microstructures play an important role in the maintenance of multipotency stem cells. However, it not clear how micropatterns affect stemness We prepared different sizes, shapes and aspect ratios used them for culture mesenchymal cells (MSCs) derived from human bone marrow to investigate their influence on MSCs at single cell level. The percentage positively stained by markers decreased with spreading area ratio. cellular geometry controlled geometrical had...
Extracellular matrix (ECM) scaffolds derived from cultured cells have drawn increasing attention for use in tissue engineering. We developed a method to prepare cell-derived ECM by combining three-dimensional cell culture, decellularization, and selective template removal. Cell-ECM-template complexes were first formed culture of poly(lactic-co-glycolic acid) (PLGA) mesh deposit their own ECM. The subsequently decellularized remove cellular components. Finally, the PLGA was selectively...
P/Ag/Ag<sub>2</sub>O/Ag<sub>3</sub>PO<sub>4</sub>/TiO<sub>2</sub> composite photocatalyst has been successfully prepared by a sol–gel method. It shows highly enhanced photocatalytic ability and stability under simulated solar light irradiation.
Electrospinning has recently emerged as a leading technique for the formation of nanofibrous structures made synthetic and natural extracellular matrix components. In this study, scaffolds were obtained by electrospinning combination poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) type-I collagen in 1,1,1,3,3,3-hexafluoro-2-isopropanol (HIFP). The resulting fibers ranged from 300 to 600 nm diameter. Their surfaces characterized attenuated total reflection Fourier transform infrared...
Elastography is a technique for detecting the stiffness of tissues. We applied elastography diagnosis prostate cancer and evaluated usefulness biopsy. The subjects this study were 311 patients who underwent during needle biopsy at Hitachi General Hospital. Strain images obtained compression tissue displayed on monitor recorded computer. elastographic moving (EMI) retrospectively. evaluable results compared in terms feasibility accuracy. median patient age was 67 years (range 50–85 years),...
An extracellular microenvironment, including an matrix (ECM), is important factor in regulating stem cell differentiation. During tissue development, the ECM dynamically remodeled to regulate functions. Here, we developed matrices mimicking remodeling during osteogenesis of mesenchymal cells (MSCs). The were prepared from cultured MSCs controlled at different stages and referred as "stepwise osteogenesis-mimicking matrices." supported adhesion proliferation showed effects on MSCs. On early...
Micropatterned surfaces are very useful to control cell microenvironment and investigate the physical effects on function. In this study, poly(vinyl alcohol) (PVA) micropatterns polystyrene cell-culture plates were prepared using UV photolithography. Cell adhesive geometries of triangle, square, pentagon, hexagon, circle surrounded by nonadhesive PVA manipulate shapes. These different had same small surface areas for spreading. Human mesenchymal stem cells (MSCs) cultured micropatterned...