A5064577946- Protein purification and stability
- Viral Infectious Diseases and Gene Expression in Insects
- Monoclonal and Polyclonal Antibodies Research
- Blood groups and transfusion
- Microfluidic and Capillary Electrophoresis Applications
- Digestive system and related health
- Polysaccharides and Plant Cell Walls
- Magnetic and Electromagnetic Effects
- Enzyme Production and Characterization
BOKU University
2018-2023
Austrian Centre of Industrial Biotechnology (Austria)
2022
Fusion protein technologies improve the expression and purification of recombinant proteins, but removal tags involved requires specific proteases. The circularly permuted caspase-2 (cpCasp2) with its cleavage site, efficiently generates untagged protein. While cpCasp2 is possible before all 20 proteinogenic amino acids, valine, leucine, isoleucine, aspartate glutamate suffers from slow, proline extremely turnover. To make platform fusion process even more general such that any an authentic...
Royal jelly has received attention because of its necessity for the development queen honeybees as well claims benefits on human health; this product hypopharyngeal glands worker bees contains a large number proteins, some which have been claimed to various biological effects only in their glycosylated state. However, although there glycomic and glycoproteomic analyses past, none glycan structures previously defined would appear potential trigger specific functions. In current study, whole...
Continuous precipitation coupled with continuous tangential flow filtration is a cost-effective alternative for the capture of recombinant antibodies from crude cell culture supernatant. The removal surge tanks between unit operations, by adoption tubular reactors, maintains harvest and mass product advantage narrow residence time distribution (RTD). We developed process implementing two orthogonal methods, CaCl
Precipitation of antibodies by means suitable precipitation agents is considered as an alternative to state-of-the-art affinity chromatography, although redissolution the precipitated product with minimal formation aggregates still poses challenges. One these find appropriate buffer composition, promoting rapid at high yields, impact on quality. Here we report development a robust method for efficient two industrially relevant antibodies, namely adalimumab and trastuzumab, which were ZnCl2....