A5089135652- Genomics and Chromatin Dynamics
- Fungal and yeast genetics research
- Epigenetics and DNA Methylation
- Plant Molecular Biology Research
- Cancer-related gene regulation
- Colorectal Cancer Treatments and Studies
- Plant Gene Expression Analysis
- Genetic factors in colorectal cancer
- Virology and Viral Diseases
- RNA Research and Splicing
- Colorectal Cancer Screening and Detection
- Ubiquitin and proteasome pathways
- Colorectal Cancer Surgical Treatments
- RNA modifications and cancer
- Gene expression and cancer classification
- Metabolism, Diabetes, and Cancer
- Growth Hormone and Insulin-like Growth Factors
- Fungal Biology and Applications
- Telomeres, Telomerase, and Senescence
- CRISPR and Genetic Engineering
- Vaccine Coverage and Hesitancy
- Polyamine Metabolism and Applications
- Fermentation and Sensory Analysis
- Cancer Treatment and Pharmacology
- Cancer Genomics and Diagnostics
University of Maryland, Baltimore
2022-2024
California State University, Sacramento
2024
University of Maryland, Baltimore County
2013-2023
University of Maryland Marlene and Stewart Greenebaum Comprehensive Cancer Center
2022-2023
Cornell University
2018-2020
Grove City College
2018
University of Maryland, College Park
2017
Stanford University
2010-2014
Mayo Clinic
2001-2012
University of California, Berkeley
2002-2012
Purpose With three available chemotherapy drugs for advanced colorectal cancer (CRC), response rate (RR) and survival outcomes have improved with associated morbidity, accentuating the need tools to select optimal individualized treatment. Pharmacogenetics identifies likelihood of adverse events or based on variants in genes involved drug transport, metabolism, cellular targets. Patients Methods Germline DNA was extracted from 520 patients North American Gastrointestinal Intergroup N9741...
OBJECTIVE—The Mayo Health System Diabetes Translation Project sought to assess models of community-based diabetes care and use a electronic management system (DEMS). Planned is redesigned model chronic disease that involves guideline implementation, support self-management, clinical information systems. RESEARCH DESIGN AND METHODS—We studied adult diabetic patients attending three primary practice sites in Wisconsin Minnesota. We implemented planned at all DEMS the 16 providers. assessed...
ABSTRACT We previously demonstrated that plasmid-deficient Chlamydia muridarum retains the ability to infect murine genital tract but does not elicit oviduct pathology because it fails activate Toll-like receptor 2 (TLR2). derived a plasmid-cured derivative of human isolate trachomatis D/UW-3/Cx, strain CTD153, which also TLR2, indicating this virulence phenotype is associated with plasmid loss in both C. and muridarum. As observed , CTD153 displayed impaired accumulation glycogen within...
Purpose Previous studies have suggested that higher plasma 25-hydroxyvitamin D 3 [25(OH)D] levels are associated with decreased colorectal cancer risk and improved survival, but the prevalence of vitamin deficiency in advanced its influence on outcomes unknown. Patients Methods We prospectively measured 25(OH)D 515 patients stage IV participating a randomized trial chemotherapy. Vitamin was defined as lower than 20 ng/mL, insufficiency to 29 sufficiency ≥ 30 ng/mL. examined association...
Purpose Racial disparities in colorectal cancer (CRC) survival are documented, but there few data on comparative response to chemotherapy. A subgroup analysis of a multisite National Cancer Institute–sponsored trial (N9741) was performed comparing outcomes black and white patients with metastatic CRC receiving uniform treatment. Patients Methods Adverse events (AEs), rate (RR), time progression (TTP), overall (OS), dose-intensity were examined as function self-reported race 1,412 treated...
<i>Objective:</i> Epidermal growth factor receptor inhibitors can result in a severe rash 5–10% of patients and detract from quality life. The objective this study was to identify clinical predictors the hope utilizing such factors design future palliative prevention trials. <i>Methods:</i> 933 cetuximab-treated enrolled on N0147, an adjuvant chemotherapy trial for colon cancer, were evaluated risk rash. <i>Results:</i> Within cohort, 50 (5%) developed...
To evaluate clinical characteristics and survival outcomes among patients with locally advanced or metastatic colorectal cancer who achieve a complete response (CR) to systemic treatment either alone multimodality approach.Data were collected retrospectively from CRC enrolled onto the phase III trial N9741, National Cancer Institute-funded Gastrointestinal Intergroup-sponsored study coordinated by North Central Treatment Group. Patients randomly assigned combinations of oxaliplatin,...
The genome is nonrandomly organized within the nucleus, but it remains unclear how gene position affects expression. Silenced genes have frequently been found associated with nuclear periphery, and environment at periphery believed to be refractory transcriptional activation. However, in budding yeast, several highly regulated classes of genes, including GAL7-10-1 cluster, are known translocate concurrent their To investigate role positioning on GAL expression, we monitored effects mutations...
Abstract Purpose: Insulin-like growth factor (IGF)-I and IGF-II stimulate neoplastic cell inhibit apoptosis, whereas IGF-binding protein-3 (IGFBP-3) inhibits the bioavailability of IGF-I has independent proapoptotic activity. We examined influence baseline plasma levels IGF-I, IGF-II, IGFBP-3, C-peptide on outcome among patients receiving first-line chemotherapy for metastatic colorectal cancer. Experimental Design: The as well data prognostic factors body size were measured at 527...
Accurate chromosome segregation is dependent on the centromere-specific histone H3 isoform known generally as CenH3, or Cse4 in budding yeast. Cytological experiments have shown that appears at extracentromeric loci yeast cells deficient for both CAF-1 and HIR H3/H4 deposition complexes, consistent with increased nondisjunction these double mutant cells. Here, we examined molecular aspects of this mislocalization. Genome-scale chromatin immunoprecipitation analyses demonstrated broader...
Despite recent advances in our ability to detect adaptive evolution involving the cis-regulation of gene expression, knowledge molecular mechanisms underlying these adaptations has lagged far behind. Across all model organisms, causal mutations have been discovered for only a handful expression adaptations, and even these, mechanistic details (e.g. trans-regulatory factors involved) not determined. We previously reported polygenic adaptation down-regulation ergosterol biosynthesis pathway...
In the presence of oxidative stress, cellular defense systems that can detoxify reactive oxygen species are activated through multiple signaling cascades and transcriptional reprogramming. The budding yeast Saccharomyces cerevisiae has served as an excellent model for genetically-identifying factors important response to stress. Here, we describe two assays testing gene deletion strains or overexpressing a interest viability following stress induced by hydrogen peroxide treatment. These...
A complex interplay between multiple chromatin modifiers is critical for cells to regulate structure and accessibility during essential DNA-templated processes such as transcription. However, the coordinated activities of these in regulation gene expression are not fully understood. We previously determined that budding yeast histone H4 methyltransferase Set5 functions together with Set1, H3K4 methyltransferase, specific cellular contexts. Here, we sought understand relationship...
We tested the hypothesis that 12 lymph node (LN) count and other surgical variables would not predict survival in a setting where techniques were standardized surgeons credentialed audited.The National Quality Forum has adopted 12-node minimum as quality metric due to strong association between survival.We performed secondary analysis of data from Clinical Outcomes Surgical Therapy (COST) multicenter randomized trial testing laparoscopic versus open colectomy. Surgeons video-audited for...
Genes adjacent to telomeres are subject transcriptional repression mediated by an integrated set of chromatin modifying and remodeling factors. The Saccharomyces cerevisiae have served as a model for dissecting the function diverse proteins in gene silencing, their study has revealed overlapping roles many either promoting or antagonizing repression. H3K4 methyltransferase Set1, which is commonly linked activation, been implicated telomere silencing. Set5 H4 K5, K8, K12 that functions with...
The Set4 protein in the yeast Saccharomyces cerevisiae contains both a PHD finger and SET domain, common signature of chromatin-associated proteins, shares sequence homology with Set3, fly UpSET, human mixed-lineage leukemia 5 (MLL5). However, biological role for its potential function chromatin regulation has not been well defined. Here, we analyzed cell phenotypes associated loss or overexpression, which revealed that protects against oxidative stress induced by hydrogen peroxide. Gene...