A5064041734- Cancer Genomics and Diagnostics
- Cancer Cells and Metastasis
- Immunotherapy and Immune Responses
- RNA Research and Splicing
- Epigenetics and DNA Methylation
- Cancer-related molecular mechanisms research
- Multiple Myeloma Research and Treatments
- Glycosylation and Glycoproteins Research
- Mosquito-borne diseases and control
- RNA modifications and cancer
- Malaria Research and Control
- Bacteriophages and microbial interactions
- Fibroblast Growth Factor Research
- Viral Infectious Diseases and Gene Expression in Insects
- Microbial infections and disease research
- Invertebrate Immune Response Mechanisms
- Glioma Diagnosis and Treatment
- RNA and protein synthesis mechanisms
- Proteoglycans and glycosaminoglycans research
- Aquaculture disease management and microbiota
- RNA Interference and Gene Delivery
National Human Genome Research Institute
2023-2025
National Institutes of Health
2023-2025
University of Copenhagen
2018-2022
Copenhagen University Hospital
2018-2022
Novo Nordisk (Denmark)
2016
Abstract Isolation of metastatic circulating tumor cells (CTCs) from cancer patients is high value for disease monitoring and molecular characterization. Despite the development many new CTC isolation platforms in last decade, their detection has remained a challenge due to lack specific sensitive markers. In this feasibility study, we present method based on binding malaria rVAR2 protein oncofetal chondroitin sulfate (ofCS). We show that efficiently captures CTCs hepatic, lung, pancreatic,...
Diffuse gliomas are the most common primary malignant brain tumor. Although extracranial metastases rarely observed, recent studies have shown presence of circulating tumor cells (CTCs) in blood glioma patients, confirming that a subset capable entering circulation. The isolation and characterization CTCs could provide non-invasive method for repeated analysis mutational phenotypic state during course disease. However, efficient detection has proven to be challenging due lack consistently...
Aberrant alternative splicing, prevalent in cancer, impacts various cancer hallmarks involving proliferation, angiogenesis, and invasion. Splicing disruption often results from somatic point mutations rewiring functional pathways to support cell survival. We introduce iSoMAs ( iSo form expression M utation As sociation), an efficient computational pipeline leveraging principal component analysis technique, explore how influence transcriptome-wide gene at the isoform level. Applying 33 types...
Early detection and monitoring of cancer progression is key to successful treatment. Therefore, much research invested in developing technologies, enabling effective valuable use non-invasive liquid biopsies. This includes the analysis circulating tumor cells (CTCs) from blood samples. Recombinant malaria protein VAR2CSA (rVAR2) binds a unique chondroitin sulfate modification present on vast majority cancers thereby holds promise as near-universal cell-targeting reagent isolate CTCs complex...
Abstract: The ability to detect several types of cancer using a non-invasive, blood-based test holds the potential revolutionize oncology screening. We mined tumor methylation array data from Cancer Genome Atlas (TCGA) covering 14 and identified two novel, broadly oc-curring markers at TLX1 GALR1. To evaluate their performance as general-ized screening approach, along with our previously reported bi-omarker, ZNF154, we rigorously assessed each marker individually or combined. Utilizing TCGA...
The ability to detect several types of cancer using a non-invasive, blood-based test holds the potential revolutionize oncology screening. We mined tumor methylation array data from Cancer Genome Atlas (TCGA) covering 14 and identified two novel, broadly-occurring markers at TLX1 GALR1. To evaluate their performance as generalized screening approach, along with our previously reported biomarker, ZNF154, we rigorously assessed each marker individually or combined. Utilizing TCGA applying...
Analysis of circulating tumor cells (CTCs) from blood samples provides a non-invasive approach for early cancer detection. However, the rarity CTCs makes it challenging to establish assays with required sensitivity and specificity. We combine highly sensitive CTC capture assay exploiting cell binding recombinant malaria VAR2CSA protein (rVAR2) detection colon-related mRNA transcripts (USH1C CKMT1A). Cancer are detected by RT-qPCR using proprietary Target Enrichment Long-probe Quantitative...
Abstract Aberrant alternative splicing is prevalent in cancer and affects most hallmarks involving proliferation, angiogenesis, invasion. Somatic point mutations can exert their cancer-driving functions via disruption. We propose “SoMAS” ( So matic M utation associated with A lternative S plicing), an efficient computational pipeline based on principal component analysis techniques, to explore the role of somatic shaping landscape both cis - trans -acting mechanisms. Applying SoMAS 33 types...
Abstract Metastasis is the leading cause of cancer-related deaths. The metastatic process requires malignant cells to break out primary tumor and travel distant sites through vasculatory system. Studies have shown that blood circulating (CTCs) can be used for patient management as CTC enumeration provides a non-invasive tool stratification monitoring disease status. Furthermore, there great interest in fully characterizing this highly subfraction cells. Various assays been developed enable...