- Enzyme Production and Characterization
- Biofuel production and bioconversion
- Animal Nutrition and Physiology
- Advancements in Battery Materials
- Probiotics and Fermented Foods
- Supercapacitor Materials and Fabrication
- Gut microbiota and health
- Microbial Metabolites in Food Biotechnology
- Studies on Chitinases and Chitosanases
- Transition Metal Oxide Nanomaterials
- Enzyme Catalysis and Immobilization
- Advanced battery technologies research
- Phytase and its Applications
- Bacteriophages and microbial interactions
- Plant Virus Research Studies
- Rabbits: Nutrition, Reproduction, Health
- Protein Hydrolysis and Bioactive Peptides
- Moringa oleifera research and applications
- Mast cells and histamine
- Plant and Fungal Interactions Research
- Cassava research and cyanide
- Antimicrobial Peptides and Activities
- Ruminant Nutrition and Digestive Physiology
- Animal Genetics and Reproduction
- Livestock and Poultry Management
China Agricultural University
2016-2025
Sanya University
2024-2025
Tibet University
2021
Feed Research Institute
2016-2018
Wuhan Textile University
2014-2017
Ministry of Education of the People's Republic of China
2015
Peking University
2007
An ultrasensitive label-free bioelectrochemical method for rapid determination of thrombin has been developed by directly detecting the redox activity adenine (A) nucleobases anti-thrombin aptamer using a pyrolytic graphite electrode. The protocol involves sandwich format. Thrombin, captured immobilzed antibody on microtiter plates, is detected aptamer−Au nanoparticle bio bar codes. A systematic optimization parameters carried out via ELAA (enzyme linked assay) performance. were released...
Abstract Development of three-dimensional nano-architectures on current collectors has emerged as an effective strategy for enhancing rate capability and cycling stability the electrodes. Herein, a novel type Ni 3 V 2 O 8 nanowires, organized by ultrathin hierarchical nanosheets (less than 5 nm) Ti foil, been obtained two-step hydrothermal synthesis method. Studies structural thermal properties as-prepared nanowire arrays are carried out their morphology changed obviously in following heat...
With rapid population expansion, urban development, and improved quality of life in most parts the world, agricultural development is playing an increasingly important role global economy. Agricultural essential for food security bringing millions people out poverty starvation to build maintain a stable society. In Asia, livestock industry crucial part has been growing at unprecedented pace last few decades. Livestock products contribute 40% total output sales, are faster than any other...
As a feed additive, xylanase has been widely applied in the of monogastric animals, which contains multiple plant polysaccharides. However, during manufacture, high pelleting temperatures challenge wild-type xylanases. The aim this study was to improve thermostability Aspergillus sulphureus acidic xylanase. According predicted protein structure, series disulphide bridges and proline substitutions were created by PCR, mutants expressed Pichia pastoris. Enzyme properties evaluated following...
Isolated Bacillus velezensis strain NA16, which produces proteases, amino acids and the transcription levels of different keratinolytic enzymes disulfide reductase genes in whole gene sequencing, was evaluated during feather degradation. The result shows under optimum fermentation conditions, chicken showed total acid concentration 7599 mg/L, degradation efficiency 99.3% at 72 h, protease activity 1058 U/mL keratinase 288 48 h. Goose 4918 mg/L (96 h), 98.9% 120 Chicken broth h high 17 acids,...
ABSTRACT Cecropin AD, a chimeric antimicrobial peptide obtained from cecropins, is effective at killing specific microorganisms. However, highly efficient expression system still needed to allow for commercial application of cecropin AD. For the exogenous we fused AD gene with small ubiquitin-like modifier (SUMO) and signal SacB, while Bacillus subtilis was constructed based on cells genetically modified by introduction an operon including isopropyl-β- d -thiogalactopyranoside...
A total of 288 crossbred (Duroc×Landrace×Yorkshire) growing pigs were used in two experiments to investigate the effects adding β-mannanase corn-soybean meal-based diets on pig performance and apparent tract digestibility (ATTD). Both lasted 28 d split into phases namely 1 14 days (phase 1) 15 2). In Exp. 1,144 weighing 23.60±1.59 kg BW assigned one four containing 0, 200, 400 or 600 U/kg β-mannanase. Increasing level increased weight gain (quadratic effect; p<0.01) feed efficiency (linear...
This study aimed to improve palm kernel cake by reducing anti-nutritional factors with enzymes and enhancing its nutritional value through microbial fermentation. It also examined the effects of these treatments on in broiler chicken diets. Palm was hydrolyzed using xylanase mannanase under various conditions. Co-fermentation Lactobacillus plantarum QZSL Saccharomyces boulardii mafic-1701 assessed different parameters. In animal experiment, 350 male Cobb chicks were divided into seven...
Introduction Galacto-oligosaccharides (GOS) are beneficial for alleviating lactose intolerance (LI). GOS have the ability to modify composition of intestinal microbiota. The development diseases could be influenced by gut Nevertheless, it remains unclear whether microbiota exerts an effect when alleviate LI, alterations in influence inflammatory response and digestion. Methods We first investigated effects on mice with established intolerance. Next, we demonstrated that prophylactic...
The efficient degradation of lignocellulose is essential for valorizing agricultural waste and reducing environmental pollution. An process requires an enzyme cocktail capable comprehensively deconstructing lignocellulosic components. In this study, the secretome Coriolopsis trogii Mafic-2001 induced by rice straw was examined, enzymatic composition reaction conditions were optimized. secreted that included ligninolytic enzymes, cellulases, hemicellulases. However, relative abundances...
Development of three-dimensional nanoarchitectures on current collectors has emerged as an effective strategy for enhancing rate capability and cycling stability the electrodes. Herein, a new type porous iron vanadate (Fe0.12V2O5) nanowire arrays Ti foil been synthesized by hydrothermal method. The as-prepared Fe0.12V2O5 nanowires are about 30 nm in diameter several micrometers length. effect reaction time resulting morphology is investigated mechanism formation proposed. As electrode...
This study evaluated the effects of Bacillus amyloliquefaciens TL106, isolated from Tibetan pigs' faeces, on growth performance, immune response, intestinal barrier function, morphology jejunum, caecum and colon, gut microbiota in mice with enterohaemorrhagic Escherichia coli (EHEC)‐induced diseases. In all, 40 female C57BL/6J were randomly divided into four groups: fed a normal diet (Control), oral administration TL106 daily (Ba), challenged EHEC O157:H7 day 15 (O157) (Ba+O157). The was...
In this study, α-L-arabinofuranosidase (AF) from Aspergillus awamori was heterologously expressed in Pichia pastoris X33, with a 1-fold increase AF activity after codon and vector optimization. remained stable at 60-65 °C displayed broad pH stability range of 2.5-8.0. It also demonstrated considerable resistance to pepsin trypsin. Furthermore, compared xylanase alone, exhibited marked synergistic effect the degradation expanded corn bran, distillers' dried grains solubles, reducing sugars by...