A5102815247- Echinoderm biology and ecology
- Marine Bivalve and Aquaculture Studies
- Aquatic life and conservation
- Protein Hydrolysis and Bioactive Peptides
- Invertebrate Immune Response Mechanisms
- Marine Biology and Environmental Chemistry
- Congenital heart defects research
- Zoonotic diseases and public health
- COVID-19 epidemiological studies
- Angiogenesis and VEGF in Cancer
- Spacecraft and Cryogenic Technologies
- Connective tissue disorders research
- Ubiquitin and proteasome pathways
- SARS-CoV-2 and COVID-19 Research
- Antimicrobial Peptides and Activities
- Zebrafish Biomedical Research Applications
Fundación Universitaria de Popayán
2022
University of Puerto Rico at Río Piedras
2006-2020
University of Puerto Rico System
2007-2014
University of Massachusetts Amherst
2013
Abstract Background Among deuterostomes, the regenerative potential is maximally expressed in echinoderms, animals that can quickly replace most injured organs. In particular, sea cucumbers are excellent models for studying organ regeneration since they regenerate their digestive tract after evisceration. However, echinoderms have been sidelined modern studies partially because of lack genome-wide profiling approaches afforded by genomic tools. For last decade, our laboratory has using...
Repair and regeneration are key processes for tissue maintenance, their disruption may lead to disease states. Little is known about the molecular mechanisms that underline repair of digestive tract. The sea cucumber Holothuria glaberrima represents an excellent model dissect characterize events during intestinal regeneration. To study gene expression profile, cDNA libraries were constructed from normal, 3-day, 7-day regenerating intestines H. glaberrima. Clones randomly sequenced queried...
Metazoan immunity is mainly associated with specialized cells that are directly involved the immune response. Nevertheless, both in vertebrates and invertebrates other organs might respond to activation participate either or indirectly ongoing process. However, most of what known about invertebrate has been restricted effector little information available on responses tissues organs. We now focus reactions intestinal tissue an echinoderm. Our study employs a non-conventional model,...
The ubiquitin proteasome system (UPS) is the main proteolytic of cells. Recent evidence suggests that UPS plays a regulatory role in regeneration processes. Here, we explore possibility involved during intestinal sea cucumber Holothuria glaberrima. These organisms can regenerate most their digestive tract following process evisceration. Initially, identified components H. glaberrima UPS, including sequences for Rpn10, β3, and ubiquitin-RPL40. Predicted proteins from mRNA showed high degree...
Mouse embryos lacking the polycomb group gene member Yin-Yang1 (YY1) die during peri-implantation stage. To assess post-gastrulation role of YY1, a conditional knock-out (cKO) strategy was used to delete YY1 from visceral endoderm yolk sac and definitive embryo. cKO display profound defects at 9.5 days post coitum (dpc), including disrupted angiogenesis in mesoderm derivatives altered epithelial characteristics endoderm. Significant changes both cell death proliferation were confined...
Abstract In recent years, transcriptomic databases have become one of the main sources for protein discovery. our studies nervous system and digestive tract regeneration in echinoderms, we identified several transcripts that attracted attention. One these molecules corresponds to a previously unidentified transcript ( Orpin ) from sea cucumber Holothuria glaberrima appeared be upregulated during intestinal regeneration. We now second highly similar sequence analyzed predicted proteins using...
In recent years, transcriptomic databases have become one of the main sources for protein discovery. our studies nervous system and digestive tract regeneration in echinoderms, we identified several transcripts that attracted attention. One these molecules corresponds to a previously unidentified transcript (Orpin) from sea cucumber Holothuria glaberrima appeared be upregulated during intestinal regeneration. We now second highly similar sequence analyzed predicted proteins using...
Holothuria glaberrima exhibits the capacity to regenerate body parts after injury or loss. This ability is mediated by an initial blastemal formation which requires proliferation and reorganization of precursor cells. Accordingly, various molecules play key roles in different aspects regeneration a part. The use microarrays high‐throughput sequencing lead our laboratory identify series new associated processes regeneration. Such case for protein Orpin. Based on EST database, we found that...
Our laboratory has pioneered the use of echinoderm Holothuria glaberrima as a novel model system to study organ regeneration. This organism can regenerate its digestive tract following evisceration. Undergraduate students from various programs, namely NSF-REU, NIH-MARC and NIGMS-MBRS have played important roles in development system. Here we present particular project an example undergraduate participation research. The partial sequence CUB-domain containing gene was obtained regenerating...
The regeneration of the intestine in sea cucumber H. glaberrima represents an excellent model to dissect and characterize organogenetic events during formation this organ. Our laboratory has been using system identify genes required for intestinal that are thought be conserved evolution. cDNA libraries were constructed from gastrointestinal tract glaberrima. correspond non-regenerating, 3day 7day regenerating animals. Random sequencing clones generated a total 5660 ESTs forming 1996 gene...