A5103264482- Microfluidic and Capillary Electrophoresis Applications
- Innovative Microfluidic and Catalytic Techniques Innovation
- Biosensors and Analytical Detection
- Advanced Biosensing Techniques and Applications
- Microfluidic and Bio-sensing Technologies
- Advanced biosensing and bioanalysis techniques
- Gene expression and cancer classification
- Photoacoustic and Ultrasonic Imaging
- Electrowetting and Microfluidic Technologies
- Advanced Chemical Sensor Technologies
- Plasmonic and Surface Plasmon Research
- Acoustic Wave Resonator Technologies
- Service-Oriented Architecture and Web Services
- Nanofabrication and Lithography Techniques
- Nanoplatforms for cancer theranostics
- Optical Imaging and Spectroscopy Techniques
- Biosimilars and Bioanalytical Methods
- Model-Driven Software Engineering Techniques
Tsinghua University
2013-2021
Indiana University School of Medicine
2015-2017
Center for Innovation
2017
Beijing Biocytogen (China)
2013
A plastic microdevice with an embedded filter paper disc was developed for rapid DNA extraction and “<italic>in situ</italic>” PCR amplification.
Plastic microfluidic devices with embedded chitosan-modified Fusion 5 filter paper (unmodified one purchased from GE Healthcare) have been successfully developed for DNA extraction and concentration, utilizing two different mechanisms capture: the physical entanglement of long-chain molecules fiber matrix electrostatic adsorption to fibers. This new method not only provided a high efficiency at pH by synergistically combining these capture together, but also resisted elution filters > 8 due...
A fully integrated and automated microsystem consisting of low-cost, disposable plastic chips for DNA extraction PCR amplification combined with a reusable glass capillary array electrophoresis chip in modular-based format was successfully developed warfarin pharmacogenetic testing. performed by adopting filter paper-based method, followed "in situ" that carried out directly the same reaction chamber without elution. products were then co-injected sizing standards into separation channels...
A fully automated microsystem consisting of a disposable DNA extraction and PCR microchip, as well compact control instrument, has been successfully developed for genetic testing hereditary hearing loss from human whole blood. were integrated into single 15-μL reaction chamber, where piece filter paper was embedded capturing genomic DNA, followed by in-situ amplification without elution. Diaphragm microvalves actuated external solenoids together with "one-way" fluidic strategy operated...
We have successfully developed an integrated microsystem that combines two plastic microchips for DNA extraction and PCR amplification with a glass capillary array electrophoresis chip together in compact control detection instrument automated forensic short tandem repeat (STR) analysis. followed by "in situ PCR" was conducted single reaction chamber of the microchip based on filter paper-based methodology. products were then mixed sizing standards injection electrode injected into...
We present an integrated microfluidic device capable of performing single-stranded DNA (ssDNA) preparation and magnetic bead-based microarray analysis with a white-light detection for detecting mutations that account hereditary hearing loss. The entire operation process, which includes loading streptavidin-coated beads (MBs) biotin-labeled polymerase chain reaction products, active dispersion the MBs binding, alkaline denaturation DNA, dynamic hybridization bead-labeled ssDNA to tag array,...
White light interference is used as a label-free method to detect nanoscale changes on surfaces. However, the signal-to-noise ratio of white very low, thus resulting in inaccurate results. In this paper, we report corrected based hyperspectral interferometry overcome shortcoming method. A platform was established, and DNA hybridization microarray constructed quantitate thickness variation molecules solid surface. We fluorescence resonance energy transfer (FRET) validate results our Compared...
A sensitive and high resolution small animal in vivo imaging system using upconversion nanoparticles (UNPs) microarrays was developed. The fluorescence tomography UNPs could achieve higher precision than that ordinary fluorophores, which theoretically explained by the finite element method (FEM). Given autofluorescence-insensitive property of UNPs, a subcutaneous detection sensitivity 0.93 × 10−4 wt% be achieved with UNP volume ∼10 μL tissue phantoms. Furthermore, fluorophore (25, 50 100 μm...
Biomolecular interactions can be detected by many established technologies such as fluorescence imaging, surface plasmon resonance (SPR)<sup>[1-4]</sup>, interferometry and radioactive labeling of the analyte. In this study, we have designed constructed a label-free, real-time sensing platform its operating imaging instrument that detects using optical phase differences from accumulation biological material on solid substrates. This system allows us to monitor biomolecular in real time...