A5044163102- Protein purification and stability
- Viral Infectious Diseases and Gene Expression in Insects
- Monoclonal and Polyclonal Antibodies Research
- Microbial Natural Products and Biosynthesis
- SARS-CoV-2 and COVID-19 Research
- Bacteriophages and microbial interactions
- Microbial Metabolic Engineering and Bioproduction
- Transgenic Plants and Applications
- Pancreatic function and diabetes
- Genetics, Bioinformatics, and Biomedical Research
- Medicinal Plant Research
- Microbial Metabolism and Applications
- Fungal and yeast genetics research
- Glycosylation and Glycoproteins Research
- COVID-19 epidemiological studies
- Genomics and Phylogenetic Studies
- COVID-19 diagnosis using AI
- Enzyme Production and Characterization
- vaccines and immunoinformatics approaches
- Biofuel production and bioconversion
- Plant Genetic and Mutation Studies
- Immunotherapy and Immune Responses
- Diet, Metabolism, and Disease
- Genetic factors in colorectal cancer
- Plant Growth and Agriculture Techniques
National Research and Innovation Agency
2022-2024
Indonesian Institute of Sciences
2014-2023
Indonesian Agency for Agricultural Research and Development
2022
Pusat Penelitian dan Pengembangan Peternakan
2022
Ministry of Health
2021
Biotechnology Research Center
2019
University of Stuttgart
2002
Gesellschaft zur Förderung von Medizin-, Bio- und Umwelttechnologien
1995
Whole-genome sequencing (WGS) has played a significant role in understanding the epidemiology and biology of SARS-CoV-2 virus. Here, we investigate use WGS Southeast East Asian countries as genomic surveillance during COVID-19 pandemic. Nottingham–Indonesia Collaboration for Clinical Research Training (NICCRAT) initiative facilitated collaboration between University Nottingham team Center Biotechnology, National Innovation Agency (BRIN), to carry out small number Indonesia using Oxford...
Background Indonesia is one of the Southeast Asian countries with high case numbers COVID-19 up to 4.2 million confirmed cases by 29 October 2021. Understanding genome SARS-CoV-2 crucial for delivering public health intervention as certain variants may have different attributes that can potentially affect their transmissibility, well performance diagnostics, vaccines, and therapeutics. Objectives We aimed investigate dynamics circulating over a 15-month period in Bogor its surrounding areas...
Indonesia is one of the most biodiverse countries in world and a promising resource for novel natural compound producers. Actinomycetes produce about two thirds all clinically used antibiotics. Thus, exploiting Indonesia’s microbial diversity actinomycetes may lead to discovery A total 422 actinomycete strains were isolated from three different unique areas tested their antimicrobial activity. Nine potent bioactive prioritized further drug screening approaches. The nine cultivated solid...
Cervical cancer caused by Human papillomavirus (HPV) is one of the most common causes death in women worldwide. Even though disease can be avoided immunization, expensive price HPV vaccines makes it hard to accessed middle-low-income countries. Thus, development generic needed address inequalities life-saving access. This study aimed develop HPV52 L1 VLP-based recombinant vaccine using Pichia pastoris expression system. The l1 gene was codon-optimized based on P. codon usage resulting CAI...
A three-dimensional model of the variable domain atrazine-specific Fab fragment K411B was constructed by molecular modeling using known structures highly homologous immunoglobulins as templates. Molecular dynamic simulations and cross-reactivity data were used to predict residues responsible for binding hapten 4-chloro-6-(isopropylamino)-1,3,5-triazine-2-(6-aminohexanecarboxylic acid) (iPr/Cl/C6) instead atrazine. Specific pockets could be defined chlorine, isopropylamino group C6-spacer...
In the past ten years, diabetes prevalence has increased rapidly in low- and middle-income countries due to lifestyle changes. This number of diabetic patients leads escalation recombinant insulin demand, which is creating a large global market. Pichia pastoris appeared as an alternative host produce proteins. It excellent qualifications expression for large-scale production proteins therapeutic use. this study, we attempted express precursor (IP) P. pastoris. We used synthetic IP-encoding...
In Indonesia, early-onset colorectal cancer (EOCRC) rates are higher in patients < 50 years old compared to Western populations, possibly due a frequency of Lynch syndrome (LS) CRC patients. We aimed examine the association KRAS and PIK3CA mutations with LS.
The recombinant yeast Pichia pastoris produced insulin precursor (IP) by secreting it extracellularly. Synthetic prepro-leaders were developed for IP secretory expression in Saccharomyces cerevisiae, and we employed a P. clone (CL-4) with synthetic IP-encoding gene its genome to generate IP. secreted was analyzed using LC-MS/MS analysis compare the theoretical molecular weight. A pulse-batch method utilized produce 2 l bioreactor. purified further converted into mature human (HI) through...
Human insulin was the first FDA-approved biopharmaceutical drug produced through recombinant DNA technology. The previous studies successfully expressed human precursors (HIP) in Pichia pastoris truncated and full-length α-factor clones. matting (Matα), a signal secretion, direct HIP protein into culture media. This study aimed to compare expression from secretory signals clones that grown two types of media, buffered methanol complex medium (BMMY) basal salt (BSMM). ImageJ analysis HIP's...
Streptomyces sp. SHP22-7 is a novel strain isolated from mangrove sample on Enggano Island, Indonesia. Here, we present the 7.9-Mbp genome sequence of SHP22-7, which will provide insight into its natural compound biosynthetic potential.
Abstract A year after the World Health Organisation (WHO) declared COVID-19 as a pandemic, much has been learned regarding SARS-CoV-2 epidemiology, vaccine production, and disease treatment. Whole-genome sequencing (WGS) played significant role in contributing to our understanding of epidemiology biology this virus. In paper, we investigate use WGS Southeast East Asia impact technological development, access resources, demography individual countries on its uptake. Using Oxford Nanopore...
Marine actinomycetes were isolated from mangrove sediment the coast in Enggano, Bengkulu Province, Indonesia using medium NBRC No. 802 modified by addition of 2% NaCl. A total 29 isolates three samples and evaluated their potential to produce bioactive metabolites. Screening marine performed against bacterial pathogens had been done. Bacteria test used was Escherichia coli 14 237, Staphylococcus aureus NBRC13 276, Bacillus subtilis 3134. result showed that seven have inhibitory effects...
Tuberculosis (TB) is an airborne illness generated by Mycobacterium tuberculosis (Mtb), also one of the prominent infectious killers adults worldwide. There a pressing need to expand novel anti-mycobacterial drugs because increasing resistance pathogenic mycobacteria existing antibiotics. Native compounds acquired from microbial resources and medicinal cultivars have played essential part as origin TB medications. The microplate resazurin reduction assay (MRRA) generally utilized assess...
Indonesia is one of the most biodiverse countries in world and a promising resource for novel natural compound producers. Actinomycetes produce about two-thirds all clinically used antibiotics. Thus, exploiting Indonesia&rsquo;s microbial diversity actinomycetes may lead to discovery A total 422 actinomycete strains were isolated from three different unique areas tested their antimicrobial activity. Nine potent bioactive prioritized further drug screening approaches. The nine cultivated...
Pichia pastoris is an alternative yeast expression system to produce heterologous proteins. It has excellent characteristics for industrial cell factory, such as its ability reach high densities, secretory capacity, and a low level of native In our previous study, we introduced synthetic insulin precursor (IP)-encoding gene constructed in pD902 vector into P. pastoris. However, the recombinant strains expressed little amount IP protein. Here, modified conditions, including inoculum density,...
Intimin is the main adhesin of Enterohemorrhagic E. coli (EHEC) O157:H7 bacteria which are most common leading infectious cause bloody diarrhea and acute kidney failure in children who develop hemolytic uremic syndrome (HUS). required for persistent bacterial colonization to eukaryotic host cell its receptor-binding activity localized at C-terminus 282 amino acids (Intimin282). Thus, Intimin282 an attractive antigen candidate that could be useful vaccine diagnostic systems against EHEC...
Abstract Co-cultivation is one of the ways to induce discovery new compounds by activating cryptic genes in a microorganism’s genome. Actinomycetes an order bacteria with many clusters gene coding for bioactive that has not yet been expressed In this study, co-cultivation between strain DHE5-1 pathogenic are conducted. Activity assay was done disk diffusion method and secondary metabolites identified using HPLC UV-Vis. The results showed activity from as antimicrobial agent against...
Tuberculosis (TB) infection is one of the primaryinfectious diseases in many developing countries; even there are minor cases some developed countries. TB spread through air and ismore probable when using improper disinfectant on medical laboratory equipment which related to research. The appropriate disinfectants commonly usedin can reduce risk transmission disease. Alcoholic compoundsare common with a broad spectrum activity towardsmicrobes,viruses, fungus. We employed molecular docking...
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Abstract Background In Indonesia, early-onset colorectal cancer (EOCRC) rates are higher in patients <50 years old compared to western populations, possibly due a frequency of Lynch Syndrome (LS) CRC patients. We aim examine the association KRAS and PIK3CA mutation with LS. Methods this cross-sectional study, PCR-HRM-based test was used for screening MSI mononucleotide markers (BAT25, BAT26, BCAT25, MYB, EWSR1), MLH1 promoter methylation, oncogene mutations BRAF(V600E), (exon 2 3), 9 20)...
Trypsin plays a crucial role in processing recombinant precursor insulins, especially removing peptide linker or spacer by cleaving the after lysine arginine residue. To reduce cell toxicity of expressed trypsin and increase secretory expression, we developed strategy for producing bovine trypsinogen using methylotrophic yeast Pichia pastoris. The expression cassette contains gene encoding truncated α-mating factor from Saccharomyces cerevisiae with cleavage site Kex2 endoprotease, short...