A5027179435- Genomics and Phylogenetic Studies
- Microbial Natural Products and Biosynthesis
- Enzyme Production and Characterization
- Plant Pathogens and Fungal Diseases
- Biofuel production and bioconversion
- Microbial Community Ecology and Physiology
- Microbial Metabolism and Applications
- Probiotics and Fermented Foods
- Food and Agricultural Sciences
- Microbial Metabolites in Food Biotechnology
- Plant Disease Resistance and Genetics
- Tannin, Tannase and Anticancer Activities
- Genetics, Bioinformatics, and Biomedical Research
- Mycorrhizal Fungi and Plant Interactions
- Microbial Metabolic Engineering and Bioproduction
- Fungal Biology and Applications
- Food Chemistry and Fat Analysis
- Seaweed-derived Bioactive Compounds
- Actinomycetales infections and treatment
- Ruminant Nutrition and Digestive Physiology
- Studies on Chitinases and Chitosanases
- Proteins in Food Systems
- Bacteriophages and microbial interactions
- Protein Hydrolysis and Bioactive Peptides
- Phytase and its Applications
National Research and Innovation Agency
2021-2024
Indonesian Agency for Agricultural Research and Development
2024
Indonesian Institute of Sciences
2011-2021
IPB University
2014
State University of Papua
2006
The most biological multiplicity on this planet is almost certainly concealed in soils. Many valuable bacteria had been extensively dispersed soils worldwide, with from terrestrial, desserts and Antarctic. Hence, become an intensively utilized ecological niche for the inhabitants to generate various useful biologically active natural products such asantibiotics, antifungal, antiviral, antioxidant, neuroprotection, anticancer other important compounds. Bacteria including Actinobacteria have...
An actinomycete strain, ID05-A0528(T), was isolated using the SDS-yeast extract pre-treatment method from soil under mahogany (Swietenia mahogani) trees in West Timor, Indonesia, and examined by a polyphasic taxonomic approach. Chemotaxonomic phylogenetic characterizations demonstrated that novel strain belongs to genus Dietzia. 16S rRNA gene sequencing studies showed related Dietzia cinnamea (97.2 %). Results of phenotypic analyses determined ID05-A0528(T) is different known species It...
Six actinomycete strains isolated from soil and plant-litter samples in Indonesia were studied for their taxonomic position by using a polyphasic approach. Phylogenetically, all the located broad cluster of genus Actinokineospora . Chemotaxonomic data [cell-wall diamino acid, meso -diaminopimelic acid; cell-wall peptidoglycan, type III (A1 γ ); major sugars, galactose arabinose; menaquinone, MK-9(H 4 fatty iso-C 16 : 0 ; phospholipid, phosphatidylethanolamine] supported affiliation six to...
Indonesia is one of the most biodiverse countries in world and a promising resource for novel natural compound producers. Actinomycetes produce about two thirds all clinically used antibiotics. Thus, exploiting Indonesia’s microbial diversity actinomycetes may lead to discovery A total 422 actinomycete strains were isolated from three different unique areas tested their antimicrobial activity. Nine potent bioactive prioritized further drug screening approaches. The nine cultivated solid...
Two actinomycete strains, ID05-A0653(T) and ID06-A0464(T), were isolated from soils of West Timor Lombok island, respectively, in Indonesia. 16S rRNA gene sequence analysis clearly demonstrated that the isolates belonged to family Pseudonocardiaceae closely related genus Actinophytocola. Strains ID06-A0464(T) exhibited 98.1 98.2 % similarity, with Actinophytocola oryzae GMKU 367(T). The grew well on ISP media produced white aerial mycelium. Short spore chains formed directly substrate...
A novel Gram-stain-positive actinobacterium, designated PS-14-7(T), was isolated from the rhizosphere of a mangrove on Pramuka Island, Indonesia, and its taxonomic position investigated using polyphasic approach. The peptidoglycan type strain PS-14-7(T) A4α lysine diagnostic diamino acid peptidoglycan. predominant menaquinone MK-8(H4) major fatty acids were anteiso-C(15 : 0), C(16 0) iso-C(16 0). DNA G+C content 72.8 mol%. Phylogenetic analysis based 16S rRNA gene sequence comparisons...
<abstract> <p>The objective of this study was to determine the characteristics fresh rice straw silage quality prepared with addition <italic>Lactiplantibacillus plantarum</italic> 1A-2 and crude cellulase alone or in combination. Quality observed through chemical composition, structure <italic>in vitro</italic> digestibility. Six treatments were used study, i.e., 1) without any treatment as control, 2) 0.1% <italic>L. (LAB1), 3) 1% (E1), 4) enzyme....
A novel spherical actinobacterium, designated RS-2-3T, was isolated from the rhizosphere of a mangrove growing on Rambut Island, Indonesia, and its taxonomic position investigated using polyphasic approach. Phylogenetic analysis based 16S rRNA gene sequence comparison revealed that strain RS-2-3T related to members genus Kocuria. The highest similarity value observed with Kocuria marina KMM 3905T (97.0 %). peptidoglycan type found be A3α an interpeptide bridge comprising l-Ala4-5....
Tea is one of the most popular beverages in world. Produced from Camellia sinensis leaves, tea has been studied to provide health benefits due content important metabolites. This study aimed investigate catechin contents, antioxidant, and antibacterial activities Indonesian varieties, namely green tea, black white tea. infusion was prepared by extracting 1 g each sample into 10 mL distilled water incubated at 80 °C for 60 min. The epigallocatechin gallate (EGCG) contents extracts were...
Tuberculosis (TB) is the foremost cause of infectious fatality globally. The primary global challenge in combatting TB lies addressing emergence drug-resistant variants disease. However, number newly approved agents for treating has remained remarkably low over recent decades. Hence, research endeavors discovering novel anti-TB are always needed. In present study, we screened 1,500 culture extracts from actinomycetes isolated Indonesia their inhibitory activity against
The taxonomic positions of actinomycete strains ID03-0915T and ID03-0825, isolated from soil on the Indonesian island Bali, were examined using a polyphasic approach. morphological chemotaxonomic characteristics these organisms are typical genus Streptomyces. Phylogenetic analyses performed almost-complete 16S rRNA gene sequences demonstrated that closely related to Streptomyces glauciniger lilacinus. However, DNA-DNA hybridization phenotypic revealed differed known species. Therefore, we...
Two actinomycete strains, designated LIPI11-2-Ac034T and LIPI11-2-Ac042T, were isolated from leaf litter collected Cibodas Botanical Garden, West Java, Indonesia. Phylogenetic analysis based on 16S rRNA gene sequences suggested that both isolates belong to the genus Actinoplanes. These closely related Actinoplanes ferrugineus durhamensis with similarity values of 98.2 % 97.7 respectively, for strain LIPI11-2-Ac034T, 99.0 97.4-97.7 respectively LIPI11-2-Ac042T. Both grew well ISP 7 medium...
Optimization of fermentation conditions is important in the development bioprocesses. Here, we used Taguchi experimental design and Response Surface Methodology to optimize medium for enhancing carboxymethyl cellulase (CMCase) production by marine actinomycetes Streptomyces sp. Bse 7-9. using found that bagasse has a significant effect on other factors. The Central Composite Design was determine interaction between factors selected obtain optimal concentration ingredients. results...
A total of 229 strains actinomycetes were isolated and identified by full sequence 16S rRNA gene analysis. Samples consisted 18 soil 20 leaf-litter collected from Eka Karya Botanical Garden, Bali Island, Indonesia. Two isolation methods, i.e. SDS-Yeast Extract (SY) Rehydration-Centrifugation (RC) used in this study. Based on analysis, may be grouped into 28 genera. molecular analysis similarities showed that Garden origin is diverse. Analysis 144 isolates samples, resulted 24 genera more...
Ensiling is one solution to handle lack of forage at dry and wet season in tropical area. Napier grass (Pennisetum purpureum) a popular crop conserved as silage, regarded with relatively high matter (DM) content adequate water soluble carbohydrates for fermentation lactic acid. The aim this study was determine the effect adding acid bacteria inoculants ensiling on quality silage. Two strain Lactobacillus plantarum 1A-2 1BL-2 were added different variation , single or mix (0%, 0.1%, 0.3%,...
Streptomyces sp. SHP22-7 is a novel strain isolated from mangrove sample on Enggano Island, Indonesia. Here, we present the 7.9-Mbp genome sequence of SHP22-7, which will provide insight into its natural compound biosynthetic potential.
A novel actinomycete strain, designated LIPI11-2-Ac046T, was isolated from a leaf litter sample obtained Cibodas Botanical Garden, West Java, Indonesia, using the rehydration and centrifugation method. The taxonomic status of this organism established polyphasic approach. Comparative 16S rRNA gene sequence analysis revealed that strain LIPI11-2-Ac046T had closest similarities with members genus Cryptosporangium (97.99-98.90 %). grew well on ISP 4 5 media formed sporangia. Spores were motile....
The strain Streptomyces sp. BSE7F, a novel isolated from Indonesian mangrove sediment, displays antimicrobial activities against Gram-positive bacteria, Gram-negative and yeast.
AbstractA total of 98 actinomycetes were isolated from the soil and litter samples collected cacao coffee plantation in Lombok Island, West Nusa Tenggara, Indonesia. These isolates screened for their antimicrobial activity. Among strains, only 24 showed activity against test microorganisms which 20.4% active Bacillus subtilis BTCC B-612, 14.3% Staphylococcus aureus B-611, 5.1% Escherichia coli B-609. Out these isolates, 3 found to be able grow medium containing mM Selenium oxide culture...
Abstract Currently, the study and development of plastic degradation are receiving great attention since it could potentially be applied to degradation. Owing its environmentally benign nature, enzymatic process is highly desired. Polyethylene terephthalate (PET) one most common materials. The PET was possible by treatment three hydrolases, namely cutinase, lipase, esterase. On other hand, marine actinomycetes, which commonly found associate with organisms such as a sponge or residing in...