A5058054591- Viral Infectious Diseases and Gene Expression in Insects
- Protein purification and stability
- Transgenic Plants and Applications
- Microbial Metabolic Engineering and Bioproduction
- Fungal and yeast genetics research
- Animal Virus Infections Studies
- Pancreatic function and diabetes
- SARS-CoV-2 and COVID-19 Research
- 3D Printing in Biomedical Research
- Polyamine Metabolism and Applications
- Healthcare and Venom Research
- Antibiotic Resistance in Bacteria
- Biological Activity of Diterpenoids and Biflavonoids
- Diabetes Management and Research
- Industrial Automation and Control Systems
- Microbial infections and disease research
- Streptococcal Infections and Treatments
- Antimicrobial Resistance in Staphylococcus
- Enzyme-mediated dye degradation
- Public Health and Nutrition
- vaccines and immunoinformatics approaches
- Proteins in Food Systems
- Biochemical and biochemical processes
- Biofuel production and bioconversion
- Virology and Viral Diseases
Indonesian Agency for Agricultural Research and Development
2022-2024
National Research and Innovation Agency
2022-2024
Indonesian Institute of Sciences
2019-2021
Airlangga University
2003
Whole-genome sequencing (WGS) has played a significant role in understanding the epidemiology and biology of SARS-CoV-2 virus. Here, we investigate use WGS Southeast East Asian countries as genomic surveillance during COVID-19 pandemic. Nottingham–Indonesia Collaboration for Clinical Research Training (NICCRAT) initiative facilitated collaboration between University Nottingham team Center Biotechnology, National Innovation Agency (BRIN), to carry out small number Indonesia using Oxford...
The recombinant yeast Pichia pastoris produced insulin precursor (IP) by secreting it extracellularly. Synthetic prepro-leaders were developed for IP secretory expression in Saccharomyces cerevisiae, and we employed a P. clone (CL-4) with synthetic IP-encoding gene its genome to generate IP. secreted was analyzed using LC-MS/MS analysis compare the theoretical molecular weight. A pulse-batch method utilized produce 2 l bioreactor. purified further converted into mature human (HI) through...
Human insulin was the first FDA-approved biopharmaceutical drug produced through recombinant DNA technology. The previous studies successfully expressed human precursors (HIP) in Pichia pastoris truncated and full-length α-factor clones. matting (Matα), a signal secretion, direct HIP protein into culture media. This study aimed to compare expression from secretory signals clones that grown two types of media, buffered methanol complex medium (BMMY) basal salt (BSMM). ImageJ analysis HIP's...
Abstract A year after the World Health Organisation (WHO) declared COVID-19 as a pandemic, much has been learned regarding SARS-CoV-2 epidemiology, vaccine production, and disease treatment. Whole-genome sequencing (WGS) played significant role in contributing to our understanding of epidemiology biology this virus. In paper, we investigate use WGS Southeast East Asia impact technological development, access resources, demography individual countries on its uptake. Using Oxford Nanopore...
Pichia pastoris is an alternative yeast expression system to produce heterologous proteins. It has excellent characteristics for industrial cell factory, such as its ability reach high densities, secretory capacity, and a low level of native In our previous study, we introduced synthetic insulin precursor (IP)-encoding gene constructed in pD902 vector into P. pastoris. However, the recombinant strains expressed little amount IP protein. Here, modified conditions, including inoculum density,...
Abstract The methylotrophic yeast, Pichia pastoris , is one of the preferred yeast hosts for recombinant protein expression. It has been developed as a potential host to express high level proteins, and achieve efficient secretion well growth very cell densities. Previously, we have obtained 19 P. clones harboring synthetic insulin precursor (IP) expression cassette integrated into their genomes through homologous recombination. To select which exhibit levels expression, conducted secreted...
Abstract Current recombinant human insulin production utilizes two major expression systems, Escherichia coli and Saccharomyces cerevisiae -based systems. Methylotropic yeast, Pichia pastoris , has appeared as a promising alternate yeast host for precursor (IP) due to its ability produce high titers, efficient secretion, growth very cell densities. Similar the S. system, P. secreted soluble IP into culture supernatant. In previous study, we have established clones harboring synthetic...
Trypsin plays a crucial role in processing recombinant precursor insulins, especially removing peptide linker or spacer by cleaving the after lysine arginine residue. To reduce cell toxicity of expressed trypsin and increase secretory expression, we developed strategy for producing bovine trypsinogen using methylotrophic yeast Pichia pastoris. The expression cassette contains gene encoding truncated α-mating factor from Saccharomyces cerevisiae with cleavage site Kex2 endoprotease, short...
Many novel bioactive compounds have been found to be most abundantly produced by sponges. Over the years, Indonesian sponges played an important role in expanding our understanding of chemical diversity and biological activity. Among those sponges, sponge genus Aaptos has shown antimicrobial Antimicrobial resistance spread infections immunocompromised population all contributed increase appearance severity these recent decades. Finding alternative drug from marine secondary metabolites will...
Abstract Saccharomyces cerevisiae and Pichia pastoris are yeast known as a potential expression system to produce recombinant protein. The full-length α-factor (α-mating factor) secretory signal of S. plays an essential role in the secretion processing mature protein interest. Here, we attempted construct sequence pD902-IP (Insulin Precursor) vector for secreted human insulin precursor P. . We have isolated pTA2 cloning vector. was then inserted into IP-cassette transformed E. coli TOP10....
Abstract Purification has an important role in obtaining protein with a high degree of purity, particularly for human therapeutic purposes. pharmaceutical proteins requires several unit operations, involving chromatographic separation techniques. To increase purification efficiency and shorten process development, it is necessary to examine the chromatography system performing capture intermediate single step. We use one best Pichia pastoris clones obtained from previous studies produce...
Production of sufficient insulin at a more affordable price is necessary. The increase in the number people living with diabetes puts burden on healthcare and economy. P. pastoris promising host to produce human precursors high yield minimal medium secretes low levels endogenous protein impurities. precursor involves several parameters, including glycerol concentration, culture density, methanol composition. This study evaluated effect those parameters expression flask culture. Subsequently,...
Abstract Streptococci are Gram-positive bacteria which cause diverse animal and human diseases systemic infections. Antibiotic therapy for this pathogen is often unsuccessful contributes to the development of antibiotic resistance. Bacteriophage modular endolysins, as well their chimeric constructs, represent promising antimicrobials with high specificity against these unlikely evoke antimicrobial Our previous work has developed recombination fusion engineered-modular endolysins design...
The objective of experiment is to know capability EDTA reduce the concentration cadmium, which accumulated in Macrobrachium sintangense organ. Two groups shrimps were exposed 30 mg/L and freshwater (control) during 7 days continue with 0.1 1 ppm days. After finish experiment, gill, hepatopancreas, carapace abdominal muscle dissected destructed concentrated HNO3 be analyzed cadmium those organs. result showed that gill control shrimp are 1.696 0.3920 mg/Kg, hepatopancreas 1.1810 0.545 carapac...