As a part of the study to explore possible strategy for enhancing shelf life mango fruits, we investigated changes in biochemical parameters and activities ripening associated enzymes Ashwina hybrid mangoes at 4-day regular intervals during storage -10°C, 4°C, 30 ± 1°C. Titratable acidity, vitamin C, starch content, reducing sugar were higher unripe state gradually decreased with increasing time all temperatures while phenol total soluble solid, sugar, nonreducing contents increased. The...

One of the most frequent neurological emergencies in Bangladesh is stroke. Poor outcome and long-term disability may result from close relatives stroke patients not being aware risk factors, symptoms, recommended for aftermath. A hospital-based cross-sectional, descriptive study was conducted to assess levels awareness among regarding management We conveniently selected 110 who were admitted into Dhaka Medical College Hospital, Dhaka, Bangladesh. The carried out between January December...

SUMMARY The epidemiological data on shigellosis in Teknaf, a coastal area of Bangladesh, were reviewed for 10-year period (1975–84). Certain similarities and differences observed the epidemiology disease Teknaf when compared with urban Dhaka rural Matlab. Similarities included: round-the-year infection two peaks, one monsoon other winter period; high male to female attendance ratio at treatment centre: predominance under-15-year age group; mortality rate under-5-year group both sexes females...

A lectin (termed NNTL) was purified from the extracts of Nymphaea nouchali tuber followed by anion-exchange chromatography on DEAE-cellulose, hydrophobic HiTrap Phenyl HP and repeated Q FF column. The molecular mass 27.0 ± 1.0 kDa, as estimated SDS/PAGE both in presence absence 2-mercaptoethanol. NNTL an o-nitrophenyl β-D-galactopyranoside sugar-specific that agglutinated rat, chicken different groups human blood cells exhibited high agglutination activity over pH range 5-9 temperatures...

A lectin (designated as KRL) was purified from the extracts of Kaempferia rotunda Linn. tuberous rhizome by glucose-sepharose affinity chromatography. KRL determined to be a 29.0 ± 1.0 kDa polypeptide SDS-PAGE under both reducing and non-reducing conditions. divalent ion dependent glycoprotein with 4% neutral sugar which agglutinated different groups human blood cells. Methyl-α-D-mannopyranoside, D-mannose methyl-α-D-glucopyranoside were most potent inhibitors. N-terminal sequence showed...

In this study, we purified and characterized an alpha-mannosidase to homogeneity from mature red tomato fruits. Purified (alpha-Man LE-1) gave two separate bands, of molecular masses 70 kDa (L-subunit) 47 (S-subunit), on SDS-PAGE under non-reducing reducing conditions. On the other hand, weight was estimated be 230 by gel filtration, indicating that alpha-Man LE-1 functions in a tetrameric structure plant cells. The N-terminal sequence L-subunit S-subunit were determined L-Y-M-V-Y-M-T-K-Q-G-...

It has been reported that acidic α-mannosidase activity increases during tomato fruit ripening, suggesting the turnover of N-glycoproteins is deeply associated with ripening. As part a study to reveal relationship between plant and maturation at molecular level, we have already purified characterized an from (Hossain et al., Biosci. Biotechnol. Biochem. 2009;73:140-146). In this article, describe identification expression gene using yeast-expression system. The α-mannosidase-gene located...

The fish products can be stored for longer period in dry condition, but most of the fresh remote areas our country are spoils due to lack proper preservation. To overcome this problem, hybrid solar dryer play an important role make drying continuous during night and daytime also off sunshine hours. In project, a cabinet has been designed, constructed, performance tested drying. consists chamber, collector, two trays dimensions (60×60) cm paraffin wax as PCM. It photovoltaic cell modules,...

Journal Article Molecular identification and characterization of an acidic peptide:N-glycanase from tomato (Lycopersicum esculentum) fruits Get access Md. Anowar Hossain, Hossain 1Department Biofunctional Chemistry, Graduate School Natural Science Technology, Okayama University, 700-8530, Japan; 2Department Biochemistry Biology, University Rajshahi, Rajshahi-6205, Bangladesh; 3Department Plant Functions Regulation, 4Kagome Research Institute, Kagome Co., Ltd., Nasu-gun, Tochigi 329-27, Japan...

A novel thermostable glucoamylase cDNA without starch binding domain (SBD) of Aspergillus flavus NSH9 was successfully identified, isolated, and overexpressed in Pichia pastoris GS115. The complete open reading frame from identified by employing PCR that encodes 493 amino acids lacking the SBD. first 17 were presumed to be a signal peptide. cloned into highest expression recombinant (rGA) observed after 8 days incubation period with 1% methanol. molecular weight purified rGA about 78 kDa...

Effects of stocking density on the growth, production, and economics all-male tilapia (Oreochromis niloticus) were investigated in a rain-fed rice-fish ecosystem for period 120 days. Fish stocked at rate 4000, 5000, 6000 ha−1 treatments T1, T2, T3, respectively. Water quality was suitable fish culture. Significantly higher growth observed T1 as compared to other treatments. SGR ranged from 1.26 1.51. Survival varied between 79% 88% with treatment producing highest survival. The benefit:cost...

Sago palm (Metroxylon sagu) is a perennial plant native to Southeast Asia and exploited mainly for the starch content in its trunk. Genetic improvement of sago extremely slow when compared other annual crops. Urgent attention needed improve planting material can be achieved through nonconventional methods. We have previously developed tissue culture method palm, which used provide materials develop genetic transformation procedure. Here, we report embryonic callus derived from suspension...

Journal Article Effect of duration diarrhoea and predictive values stool leucocytes red blood cells in the isolation different serogroups or serotypes Shigella Get access Md Anowar Hossain, Hossain ∗ International Centre for Diarrhoeal Disease Research, Bangladesh (ICDDR,B), Dhaka-1000, ∗Please address correspondence to Dr Clinical Laboratory, ICDDR,B, GPO Box 128, Bangladesh. Search other works by this author on: Oxford Academic PubMed Google Scholar M.John Albert Transactions The Royal...

beta-D-N-Acetylhexosaminidase, a family 20 glycosyl hydrolase, catalyzes the removal of β-1,4-linked N-acetylhexosamine residues from oligosaccharides and their conjugates. We constructed phylogenetic tree β-hexosaminidases to analyze evolutionary history predicted functions plant hexosaminidases. Phylogenetic analysis reveals complex evolution β-hexosaminidase that can be described by gene duplication events. The 3D structure tomato (β-Hex-Sl) was homology modeling using 1now as template....

Fructose-1,6-bisphosphate aldolase (FBAld) is an enzyme that catalyzes the cleavage of D-fructose-1,6-phosphate (FBP) to D-glyceraldehyde-3-phosphate (G3P) and dihydroxyacetone phosphate (DHAP), plays vital role in glycolysis gluconeogenesis. However, molecular characterization functional roles FBAld remain unknown sago palm. Here we report a modified CTAB-RNA extraction method was developed for isolation good quality RNA (RIN>8) from leaves cDNA The isolated (msFBAld) has total length 1288...

In this study, we identified a gene in tomato that encodes an acidic α-fucosidase (LOC101254568 or Solyc03g006980, α-Fuc'ase S1-1), which may be involved the turnover of plant complex-type N-glycans. Recombinant S1-1 (rFuc'ase S1-1) was expressed using baculovirus-insect cell expression system. rFuc'ase Sl-1 is 55 kDa size and has optimum pH around 4.5. It substantially hydrolyzed non-reducing terminal α1,3-fucose residue on LNFP III α1,4-fucose residues Lea epitopes N-glycans, but not...

AbstractPreliminary survey revealed that 30–40% of Cavendish banana plants were affected with leaf spot disease. Diseased samples sterilized 3% sodium hypochlorite solution for 2 min and cultured on PDA media at 25 °C 15 days followed by microscopic examination. Combined morphological, cultural examinations identified two causal agents designated here as BaFI-1 BaFI-2. Further, BLASTn search using internal transcribed spacer (ITS) rDNA sequences the isolates also BaIF-1 BaFI-2 had 97.13%...

Beta-galactosidase (BGAL) is an exoglycosidase that catalyses the hydrolysis of terminal β-linked galactose residues. To better understand molecular characteristics and structural insights mango BGAL (MiBGAL), we performed sequence analyses, reconstruction evolutionary tree, homology modelling docking. BGALs are widely distributed enzymes evolved from a common bacterial ancestor. Plant (pBGALs) belong to glycosyl hydrolase-35(GH35) family had close similarities with fungi BGALs. Three...

In a previous study, we molecular-characterized tomato (Solanum lycopersicum) α1, 3/4-fucosidase (α-Fuc'ase Sl-1) encoded in gene (Solyc03g006980), indicating that α-Fuc'ase Sl-1 is involved the turnover of Lea epitope-containing N-glycans. this have characterized another (Solyc11g069010) encoding Sl-2), which also active toward complex type N-glycans containing epitope(s). The baculovirus-insect cell expression system was used to express Sl-2 with anti-FLAG tag, and product (rFuc'ase found...