A5043967154- Cancer Immunotherapy and Biomarkers
- Molecular Biology Techniques and Applications
- MicroRNA in disease regulation
- Cancer Genomics and Diagnostics
- Gene expression and cancer classification
- Histone Deacetylase Inhibitors Research
- Immune cells in cancer
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Protein Degradation and Inhibitors
- Cancer Cells and Metastasis
- Pancreatic and Hepatic Oncology Research
- Renal Transplantation Outcomes and Treatments
- Cancer Research and Treatments
- Organ Transplantation Techniques and Outcomes
- RNA modifications and cancer
- CAR-T cell therapy research
- Cancer Diagnosis and Treatment
- Cancer-related Molecular Pathways
- Genetics, Aging, and Longevity in Model Organisms
- RNA Research and Splicing
- Tumors and Oncological Cases
- Cancer-related gene regulation
- Lung Cancer Treatments and Mutations
- Renal and related cancers
- Erythrocyte Function and Pathophysiology
Bernhardt Laboratories
2019-2023
Virginia Commonwealth University
2012-2021
John Wiley & Sons (United States)
2016-2018
Hudson Institute
2016-2018
Massachusetts General Hospital
2007-2018
University Health Network
2016-2018
Università Cattolica del Sacro Cuore
2017-2018
Charles River Laboratories (Netherlands)
2018
International Association for the Study of Lung Cancer
2018
College of American Pathologists
2018
Section:ChooseTop of pageAbstract <<Materials and MethodsResultsDiscussionReferencesCITING ARTICLES
Malignancies found in unexpected locations or with poorly differentiated morphologies can pose a significant challenge for tissue of origin determination. Current histologic and imaging techniques fail to yield definitive identification the number cases. The aim this study was validate predefined 1,550-gene expression profile purpose.Four institutions processed 547 frozen specimens representing 15 tissues using oligonucleotide microarrays. Half were metastatic tumors, remainder being...
Low-oxygen tolerance is supported by an adaptive response that includes a coordinate shift in metabolism and the activation of transcriptional program driven hypoxia-inducible factor (HIF) pathway. The precise contribution HIF-1a response, however, has not been determined. Here, we investigate how HIF influences hypoxic adaptation throughout Drosophila melanogaster development. We find hypoxic-induced changes are comprised HIF-dependent HIF-independent pathways distinct separable. show...
Abstract Estrogen receptor-α (ERα)-negative breast cancer is clinically aggressive and does not respond to conventional hormonal therapies. Strategies that lead re-expression of ERα could sensitize ERα-negative cancers selective ER modulators. FTY720 (fingolimod, Gilenya), a sphingosine analog, the Food Drug Administration (FDA)-approved prodrug for treatment multiple sclerosis also has anticancer actions are yet well understood. We found phosphorylated in cells by nuclear kinase 2...
Abstract Purpose: The purpose of this study is to define the mechanistic basis for recovery proliferative capacity in breast tumor cells after chemotherapy. Here, we test hypothesis that evasion senescence confers resistance chemotherapeutic drugs and ionizing radiation. Experimental Design: MCF-7 were treated with a single, clinically relevant dose (0.75-1.0 μmol/L) Adriamycin. Two weeks following induction senescence, clonal outgrowths expanded characterized terms senescence-associated...
Recent observations suggest that immune-mediated tissue destruction is dependent upon coordinate activation of immune genes expressed by cells the innate and adaptive systems. Here, we performed a retrospective pilot study to investigate whether expression molecular signature mostly associated with NK could be used segregate breast cancer patients into relapse relapse-free outcomes. By analyzing primary specimens derived from who experienced either 58–116 months (~5-9 years) survival or...
TWO MOST COMMONLY USED ANIMAL MODELS FOR STUDYING BREAST CANCER LUNG METASTASIS ARE: lung metastasis after orthotopic implantation of cells into the mammary gland, and implantations produced tail vein (TV) injection cells. Tail can produce lesions faster, but little has been studied regarding differences between these tumors, thus, we examined their morphology gene expression profiles.Syngeneic murine adenocarcinoma, 4T1-luc2 cells, were implanted either subcutaneously (Sq), orthotopically...
Alcohol consumption is known to lead gene expression changes in the brain. After performing weighted co-expression network analyses (WGCNA) on genome-wide mRNA and microRNA (miRNA) Nucleus Accumbens (NAc) of subjects with alcohol dependence (AD; N = 18) matched controls (N 18), six three miRNA modules significantly correlated AD were identified (Bonferoni-adj. p≤ 0.05). Cell-type-specific transcriptome revealed two be enriched for neuronal specific marker genes downregulated AD, whereas...
Development of quality-control criteria to ensure reproducibility microarray results for potential clinical application is still in its infancy.In the present studies we developed and evaluated their effect data analysis using total RNA from cell lines, frozen tumors, a commercially available reference RNA. Quality-control such as A(260)/A(280) ratios, percentage rRNA, median size cDNA cRNA synthesis products were robustness analysis. Furthermore, precision material performed on Affymetrix...
Induction of tumor-specific immune responses results in the inhibition tumor development. However, tumors recur because immunoediting process that facilitates development escape mechanisms tumors. It is not known whether an active whereby anti-tumor induce loss or downregulation target antigen antigen-positive clones. To address this question, we used rat neu-overexpressing mouse mammary carcinoma (MMC) and its relapsed neu antigen-negative variant (ANV). ANV emerged from MMC under pressure...
Hepatocellular carcinoma (HCC) is a highly aggressive cancer with no currently available effective treatment. Understanding of the molecular mechanism HCC development and progression imperative for developing novel, effective, targeted therapies this lethal disease. In article, we document that cellular transcription factor Late SV40 Factor (LSF) plays an important role in pathogenesis. LSF protein was significantly overexpressed human cells compared to normal hepatocytes. 109 patients,...
Astrocyte elevated gene-1 (AEG-1) is a key contributor to hepatocellular carcinoma (HCC) development and progression. To enhance our understanding of the role AEG-1 in hepatocarcinogenesis, transgenic mouse with hepatocyte-specific expression (Alb/AEG1) was developed. Treating Alb/AEG-1, but not wild-type (WT) mice, N-nitrosodiethylamine resulted multinodular HCC steatotic features associated modulation genes regulating invasion, metastasis, angiogenesis, fatty acid synthesis. Hepatocytes...
Data are accumulating to support a role for adipose-derived mesenchymal stem cells (MSCs) in breast cancer progression; however, date most studies have relied on adipose MSCs from non-breast sources. There is particular need investigate the of pathogenesis basal-like cancer, which develops at disproportionate rate pre-menopausal African-American women with gain adiposity. The aim this study was better understand how (bMSCs) contribute progression cancers by relying isogenic HMT-3255 S3...
Right ventricular failure (RVF) is the most frequent cause of death in patients with pulmonary arterial hypertension (PAH); however, specific therapies targeted to treat RVF have not been developed. Chronic treatment carvedilol has shown reduce established maladaptive right ventricle (RV) hypertrophy and improve RV function experimental PAH. However, mechanisms by which improves are unknown. We previously demonstrated microarray analysis that characterized a distinct gene expression profile...
Key Points CHD4 depletion sensitizes AML cells but not normal CD34+ progenitors to genotoxic agents by relaxing chromatin and impairing DSB repair. modulates expression of cell genes that regulate tumor formation in vivo colony vitro.