Dustin G. Wilkerson

ORCID: 0000-0003-3996-7430
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About
Contact & Profiles
Research Areas
  • Bioenergy crop production and management
  • Genetic Mapping and Diversity in Plants and Animals
  • Yeasts and Rust Fungi Studies
  • Cannabis and Cannabinoid Research
  • Wheat and Barley Genetics and Pathology
  • Plant Virus Research Studies
  • Plant tissue culture and regeneration
  • Forest Management and Policy
  • Lipid metabolism and biosynthesis
  • GABA and Rice Research
  • Toxin Mechanisms and Immunotoxins
  • Chromosomal and Genetic Variations
  • Plant and Fungal Interactions Research
  • Plant Pathogens and Fungal Diseases
  • Horticultural and Viticultural Research
  • Plant Parasitism and Resistance
  • Rice Cultivation and Yield Improvement
  • Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
  • Genetic and phenotypic traits in livestock
  • Plant Pathogens and Resistance
  • Powdery Mildew Fungal Diseases
  • Research in Cotton Cultivation
  • Biofuel production and bioconversion

Cornell University
2020-2025

Genomic characterization of Cannabis sativa has accelerated rapidly in the last decade as sequencing costs have decreased and public private interest species increased. Here, we present seven new chromosome-level haplotype-phased genomes C. sativa. All these genotypes were alive at time publication, several numerous years associated phenotype data. We performed a k-mer-based pangenome analysis to contextualize assemblies within over 200 existing assemblies. This allowed us identify unique...

10.3390/ijms26031165 article EN International Journal of Molecular Sciences 2025-01-29

Grapevine downy mildew, caused by the oomycete pathogen Plasmopara viticola, can lead to economically significant losses in humid climates. An ever-growing catalog of loci for resistance P. viticola is available breeders, including Rpv3 on lower arm chromosome 18. Widely used French-American cultivars, a complex TIR-NBS-LRR locus which associated SSR markers have provided evidence multiple alleles with varying degrees resistance. However, SSRs lack resolution detect nuances between and fully...

10.20870/oeno-one.2025.59.1.8244 article EN cc-by OENO One 2025-03-11

Powdery mildew (PM) in Cannabis sativa is most frequently caused by the biotrophic fungus Golovinomyces ambrosiae. Based on previously characterized variation susceptibility to PM, biparental populations were developed crossing resistant cultivar evaluated, ‘FL 58’, with a susceptible cultivar, ‘TJ's CBD’. F 1 progeny evaluated and displayed range of susceptibility, two self-pollinated generate 2 populations. In 2021, ( n = 706) inoculated PM surveyed for disease severity. both populations,...

10.1094/mpmi-04-23-0043-r article EN cc-by-nc-nd Molecular Plant-Microbe Interactions 2023-09-26

The Salicaceae, including Populus and Salix, are dioecious perennials that utilize different sex determination systems. This family provides a useful system to better understand the evolution of dioecy chromosomes. Here, rare monoecious genotype Salix purpurea, 94003, was self- cross-pollinated progeny ratios were used test hypotheses on possible mechanisms determination. To delimit genomic regions associated with expression, 94003 genome sequence assembled DNA- RNA-Seq inflorescences...

10.1111/nph.18853 article EN New Phytologist 2023-03-03

Abstract Within the genus Salix, there are approximately 350 species native primarily to northern hemisphere and adapted a wide range of habitats. This diversity can be exploited mine novel alleles conferring variation important for production as bioenergy crop, but also identify evolutionarily genes, such those involved in sex determination. To leverage this diversity, we created mapping population by crossing 6 Salix (Salix viminalis, suchowensis, integra, koriyanagi, udensis, alberti)...

10.1093/g3journal/jkac071 article EN cc-by G3 Genes Genomes Genetics 2022-03-25

Hemp ( Cannabis sativa L.) is a primarily short-day crop grown for grain, fiber, and secondary metabolites. Although terminal flowering of most non-domesticated hemp regarded as photoperiod sensitive, limited germplasm available the development day-neutral hemp. Day-neutral plants experience flower maturation independently cue which typically triggered by photoperiods less than 14 hours. The trait subject increased commercial interest purpose breeding varieties with accelerated time or...

10.1101/2023.04.17.537043 preprint EN cc-by-nc-nd bioRxiv (Cold Spring Harbor Laboratory) 2023-04-18

Melampsora spp. rusts are the greatest pathogen threat to shrub willow (Salix spp.) bioenergy crops. Genetic resistance is key limit effects of these foliar diseases on host response and biomass yield, however, genetic basis has not been characterized. The addition new genomic resources for Salix provides greater power investigate interaction between S. purpurea M. americana, species commonly found in Northeast US. Here, we utilize 3' RNA-seq host-pathogen interactions following controlled...

10.1186/s12864-021-08254-1 article EN cc-by BMC Genomics 2022-01-22

ABSTRACT The first step in trait introgression is to identify and assess novel sources of variation. For shrub willow ( Salix ) breeders, there an abundance understudied species within a genus that readily hybridizes. Breeding targets center on traits contributing biomass yield for bioenergy. These include stem biomass, insect pathogen resistance, leaf architecture traits. More specifically, breeding durable resistance rust Melampsora spp.) particular importance as the can significantly...

10.1111/gcbb.70002 article EN cc-by GCB Bioenergy 2024-09-17

Genomic characterization of Cannabis sativa has accelerated rapidly in the last decade as sequencing costs have decreased and public private interest species increased. Two emerging challenges for C. research community are to 1) comprehensively capture genomic diversity 2) reconcile leverage large amounts data make meaningful contributions fundamental commercial production systems. Here, we present seven new chromosome-level haplotype-phased genomes sativa. All these genotypes were alive at...

10.20944/preprints202411.1840.v1 preprint EN 2024-11-25

Shrub willows (Salix spp.) are emerging as a viable lignocellulosic, second-generation bioenergy crop with many growth characteristics favorable for marginal lands in New York State and surrounding areas. Willow rust, caused by members of the genus Melampsora, is most limiting disease shrub willow this region remains extremely understudied. In study, genetic diversity, structure, pathogen clonality were examined Melampsora americana over two growing seasons via genotyping-by-sequencing to...

10.1094/phyto-05-21-0201-r article EN Phytopathology 2021-09-28

Melampsora rust is a devastating disease of shrub willow in North America. Previous work has identified paradoxa as one two species New York State that infect Salix purpurea and other important host species, however little known about the population this region. Genotyping-by-sequencing was used to identify single nucleotide polymorphisms (SNPs) assess diversity M. isolates collected from three breeding populations Geneva, NY between 2015 2020. Statistical analyses SNP revealed all were...

10.3390/plants11182385 article EN cc-by Plants 2022-09-13

Abstract Background: Melampsora spp. rusts are the greatest pathogen threat to shrub willow ( Salix spp.) bioenergy crops. Genetic resistance is key limit effects of these foliar diseases on host response and biomass yield, however, genetic basis has not been characterized. The addition new genomic resources for provides greater power investigate interaction between S. purpurea M. americana , species commonly found in Northeast US. Here, we utilize 3' RNA-seq host-pathogen interactions...

10.21203/rs.3.rs-592685/v1 preprint EN cc-by Research Square (Research Square) 2021-06-16

ABSTRACT Within the genus Salix , there are approximately 350 species native primarily to northern hemisphere and adapted a wide range of habitats. This diversity can be exploited mine novel alleles conferring variation important for production as bioenergy crop, but also identify evolutionarily genes, such those involved in sex determination. To leverage this diversity, we created mapping population by crossing six ( S. viminalis, suchowensis, integra, koriyanagi, udensis alberti ) common...

10.1101/2021.11.04.467334 preprint EN cc-by-nc-nd bioRxiv (Cold Spring Harbor Laboratory) 2021-11-05
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