A5032553978- RNA and protein synthesis mechanisms
- Microbial Metabolism and Applications
- Antimicrobial Resistance in Staphylococcus
- Bacterial Genetics and Biotechnology
- Streptococcal Infections and Treatments
- Melanoma and MAPK Pathways
- Pneumonia and Respiratory Infections
- Peptidase Inhibition and Analysis
- Lipid Membrane Structure and Behavior
- Cancer Research and Treatments
- interferon and immune responses
- Inflammasome and immune disorders
- Bacterial Infections and Vaccines
- Cell death mechanisms and regulation
- Genomics and Phylogenetic Studies
- Plant-Microbe Interactions and Immunity
- Bacterial biofilms and quorum sensing
- Autophagy in Disease and Therapy
- HER2/EGFR in Cancer Research
- Ion Transport and Channel Regulation
- Peroxisome Proliferator-Activated Receptors
- demographic modeling and climate adaptation
- Vibrio bacteria research studies
- Ubiquitin and proteasome pathways
- Water Quality and Resources Studies
St. Jude Children's Research Hospital
2018-2023
University of Tennessee at Knoxville
2017-2022
The University of Texas at Tyler
2009
utilizes the fatty acid (FA) kinase system to activate exogenous FAs for membrane synthesis. We developed a lipidomics workflow determine phosphatidylglycerol (PG) molecular species synthesized by
Fatty acid (FA) kinase produces acyl-phosphate for the synthesis of membrane phospholipids in Gram-positive bacterial pathogens. FA consists a protein (FakA) that phosphorylates an substrate bound to second module, FA-binding (FakB). Staphylococcus aureus expresses two distinct, but related, FakBs with different selectivities. Here, we report structures FakB1 four saturated FAs at 1.6-1.93 Å resolution. We observed are accommodated within slightly curved hydrophobic cavity whose length is...
The assembly of chemotaxis receptors and signaling proteins into polar arrays is universal in motile chemotactic bacteria. Comparative genome analyses indicate that most bacteria possess multiple systems, experimental evidence suggests from distinct systems integrated. Here, we identify one such mechanism. We show paralogs two assemble together chemoreceptor arrays, forming baseplates comprised both systems. These mixed provide a straightforward mechanism for signal integration coordinated...
Fatty acid (FA) transfer proteins extract FA from membranes and sequester them to facilitate their movement through the cytosol. Detailed structural information is available for these soluble protein–FA complexes, but structure of protein conformation responsible exchange at membrane unknown. Staphylococcus aureus FakB1 a prototypical bacterial that binds palmitate within narrow, buried tunnel. Here, we define conformational change "closed" state an "open" associates with provides path entry...
Adaptation of bacteria to their host environment is a key component colonization and pathogenesis. As an essential bacterial membranes, fatty acid composition contributes adaptation.
Chemotaxis is the movement of cells in response to gradients diverse chemical cues. Motile bacteria utilize a conserved chemotaxis signal transduction system bias their motility and navigate through gradient. A central regulator histidine kinase CheA. This cytoplasmic protein interacts with membrane-bound receptors, which assemble into large polar arrays, propagate signal. In alphaproteobacterium Azospirillum brasilense, Che1 controls transient increases swimming speed during chemotaxis, but...
Abstract Bacteria of the genus Azospirillum , including most comprehensively studied brasilense are non‐pathogenic soil bacteria that promote growth diverse plants, making them an attractive model to understand non‐symbiotic, beneficial plant‐bacteria associations. Research into physiology and genetics these organisms spans decades a range molecular tools protocols have been developed for allelic exchange mutagenesis, in trans expression genes, fusions reporter genes. © 2017 by John Wiley...
Eukaryotic homologs of the progestin and AdipoQ receptor family (PAQR) have been shown to regulate membrane fluidity by affecting, through unknown mechanisms, unsaturated fatty acid (FA) metabolism. The bacterial studied here mediate small consistent changes in FA metabolism that do not seem impact but, rather, alter energetics homeostasis.
Abstract Fatty acid (FA) transfer proteins extract FA from membranes and sequester their ligand to facilitate its movement through the cytosol. While detailed views of soluble protein-FA complexes are available, how exchange occurs at membrane has remained unknown. Staphylococcus aureus FakB1 is a prototypical bacterial protein that binds palmitate within narrow, buried tunnel. Here, we determine conformational change this closed state an open engages phospholipid bilayer. Upon binding,...
The Fatty acid kinase (Fak) system is a two‐protein allowing Gram‐positive pathogens to incorporate exogenous fatty acids (FA) for membrane phospholipid synthesis. Exogenous FA bind binding protein, FakB, are phosphorylated by kinase, FakA, and used construct the membrane. Phosphorylated can also be interconverted acyl‐acyl carrier via phosphate acyltransferase PlsX, elongated synthase type II (FASII) system. We combination of cellular labeling experiments, genetics, biochemistry...
ABSTRACT Membrane lipid homeostasis is required for bacteria to survive in a spectrum of host environments. This achieved by regulation fatty acid chain length and the ratio saturated unsaturated acids. In pathogen Streptococcus pneumoniae , biosynthesis encoded cluster ( fab ) genes (FASII locus) whose expression controlled FabT repressor. Encoded immediately downstream FASII locus BriC, competence-induced, cell-cell communication peptide that promotes biofilm development as well...