A5000677164- Research on Leishmaniasis Studies
- Assisted Reproductive Technology and Twin Pregnancy
- Prenatal Screening and Diagnostics
- Trypanosoma species research and implications
- Reproductive Health and Technologies
- Micro and Nano Robotics
- Biochemical and Molecular Research
- Reproductive Biology and Fertility
- Reproductive System and Pregnancy
- Bacterial biofilms and quorum sensing
- Microbial Community Ecology and Physiology
- Cancer therapeutics and mechanisms
- Enzyme function and inhibition
- Bacterial Genetics and Biotechnology
- Endometriosis Research and Treatment
- Protist diversity and phylogeny
- Ectopic Pregnancy Diagnosis and Management
- Oral microbiology and periodontitis research
- Plant Pathogenic Bacteria Studies
- Pregnancy and Medication Impact
- Legume Nitrogen Fixing Symbiosis
- Pneumocystis jirovecii pneumonia detection and treatment
- Chemical Reactions and Mechanisms
- Hemoglobin structure and function
- Indoor Air Quality and Microbial Exposure
Icahn School of Medicine at Mount Sinai
1997-2023
Reproductive Medicine Associates of New York
2003-2023
University of Tennessee at Knoxville
2015-2021
Institute of Genomics and Integrative Biology
2011-2015
University of Delhi
2011-2015
Indian Institute of Chemical Biology
1990-2007
Mount Sinai Medical Center
2006
Infectious Disease Consultants
2004
Jadavpur University
1986
University of Calcutta
1964
Leishmania, a unicellular trypanosomatid protozoan parasite, causes wide range of human diseases ranging from the localized self-healing cutaneous lesions to fatal visceral leishmaniasis. However, it undergoes process programmed cell death during treatment with topoisomerase I poison camptothecin (CPT). The present study shows that CPT-induced formation reactive oxygen species increases level cytosolic calcium through release ions intracellular stores as well by influx extracellular calcium....
Bacterial taxonomy and phylogeny based on rrs (16S rDNA) sequencing is being vigorously pursued. In fact, it has been stated that novel biological findings are driven by comparison integration of massive data sets. spite a large reservoir 1,237,963 entries, this analysis invariably needs supplementation with other genes. The need to divide the genetic variability within taxa or genus at their phylogenetic boundaries discover those fundamental features, which will enable bacteria naturally...
The genomes of most motile bacteria encode two or more chemotaxis (Che) systems, but their functions have been characterized in only a few model systems. Azospirillum brasilense is soil alphaproteobacterium able to colonize the rhizosphere cereals. In response an attractant, A. cells transiently increase swimming speed and suppress reversals. Che1 pathway was previously shown regulate changes speed, it has minor role root surface colonization. Here, we show that second system, named Che4,...
ABSTRACT The ability of bacteria to monitor their metabolism and adjust behavior accordingly is critical maintain competitiveness in the environment. motile microaerophilic bacterium Azospirillum brasilense navigates oxygen gradients by aerotaxis order locate low concentrations that can support metabolism. When cells are exposed elevated levels surroundings, A. implement an alternative response form transient clumps cell-to-cell interactions. Clumping was suggested represent a protecting...
We have cloned and expressed the 43 kDa N-terminal domain of Leishmania donovani topoisomerase II. This protein has an intrinsic ATPase activity obeys Michaelis–Menten kinetics. Cross-linking studies indicate that exists as a dimer both in presence absence nucleotides. Etoposide, effective antitumour drug, traps eukaryotic DNA II covalent complex with DNA. In present study, we report for first time etoposide inhibits recombinant L. modelled structure this performed molecular docking analysis...
Bacterial chemotaxis is the directed movement of motile bacteria in gradients chemoeffectors. This behavior mediated by dedicated signal transduction pathways that couple environment sensing with changes direction rotation flagellar motors to ultimately affect motility pattern. Azospirillum brasilense uses two distinct pathways, named Che1 and Che4, four different response regulators (CheY1, CheY4, CheY6, CheY7) control swimming pattern during chemotaxis. Each CheY homologs was shown...
Experiments from other laboratories conducted with <i>Leishmania donovani</i> promastigote cells had earlier indicated that the plasma membrane Mg<sup>2+</sup>-ATPase of parasite is an extrusion pump for H<sup>+</sup>. Taking advantage pellicular microtubular structure organism, we report procedures obtaining sealed ghost and everted vesicle defined polarity. Rapid influx H<sup>+</sup>into vesicles was found to be dependent on simultaneous presence ATP (1 mm) Mg<sup>2+</sup> mm). Excellent...
Abstract Bacteria of the genus Azospirillum , including most comprehensively studied brasilense are non‐pathogenic soil bacteria that promote growth diverse plants, making them an attractive model to understand non‐symbiotic, beneficial plant‐bacteria associations. Research into physiology and genetics these organisms spans decades a range molecular tools protocols have been developed for allelic exchange mutagenesis, in trans expression genes, fusions reporter genes. © 2017 by John Wiley...
Controlled exposure of Leishmania donovanipromastigotes to hypotonic shock results in the formation deflagellated unsealed ghosts original polarity that largely retain pellicular microtubular structure associated with plasma membrane parasite. Gentle shearing followed by suspension purified appropriate isotonic buffer containing Mg2+ (4 mM) sealed everted vesicles. The presence appears be essential for efficient sealing and also prevent leakiness. ATP-dependent Ca2+ accumulation can...
INTRODUCTION: There is a need for evidence-based counseling women with chronic liver disease (LD) who may experience impaired fertility. Currently, the literature on assisted reproductive technology (ART) treatment in LD has been limited to single European case series. We evaluated ART outcomes patients and compared controls. METHODS: The retrospective study without had normal ovarian reserve underwent high-volume fertility practice from 2002 2021. RESULTS: identified 295 (mean age 37.8 ±...
Abstract Bacteria of the genus A. brasilense are motile and capable chemotaxis aerotaxis (taxis in gradient oxygen) using a single polar flagellum that propels cells aqueous environments. Responses to attractants repellents have been described spatial assays permit visualization these responses detailed this unit. These simple can be readily implemented with minimum set ups. © 2018 by John Wiley & Sons, Inc.