A5018704996- Arsenic contamination and mitigation
- Pituitary Gland Disorders and Treatments
- Glioma Diagnosis and Treatment
- Yersinia bacterium, plague, ectoparasites research
- Chromium effects and bioremediation
- Plant-Microbe Interactions and Immunity
- Heavy metals in environment
- Trypanosoma species research and implications
- Pharmacological Effects of Natural Compounds
- Research on Leishmaniasis Studies
- Drug Transport and Resistance Mechanisms
- Phytochemistry and Bioactive Compounds
- Enzyme function and inhibition
- Cellular transport and secretion
- Heat shock proteins research
- Cancer, Hypoxia, and Metabolism
- Spine and Intervertebral Disc Pathology
- Natural product bioactivities and synthesis
- Phytochemical Studies and Bioactivities
- Bacillus and Francisella bacterial research
- Fungal and yeast genetics research
- Medicinal Plants and Neuroprotection
- Quantum Information and Cryptography
- Aquaculture Nutrition and Growth
- Mine drainage and remediation techniques
University of Kalyani
1990-2025
Indian Institute of Information Technology Allahabad
2025
National Institutes of Health
2022-2024
Maharaja Ranjit Singh Punjab Technical University
2019-2024
Tetra Tech (United States)
2024
National Institute of Neurological Disorders and Stroke
2022-2024
Indian Institute of Chemical Biology
1997-2016
Uniformed Services University of the Health Sciences
2006-2012
Johns Hopkins Medicine
2000-2003
Johns Hopkins University
2000-2003
The discovery and biochemical characterization of the secretory pathway Ca2+-ATPase, PMR1, inSaccharomyces cerevisiae, has paved way for identification PMR1 homologues in many species including rat,Caenorhabditis elegans, Homo sapiens. In yeast, been shown to function as a high affinity Ca2+/Mn2+ pump localized Golgi compartment where it is important protein sorting, processing, glycosylation. However, little known about higher organisms. Loss one functional allele human gene, hSPCA1, linked...
We have solubilized and purified the histidine-tagged yeast secretory pathway/Golgi ion pump Pmr1 to near homogeneity in one step, using nickel affinity chromatography. The demonstrates both Ca2+- Mn2+-dependent ATP hydrolysis phosphoenzyme intermediate formation forward (ATP) reverse (Pi) directions. This preparation has allowed us examine, detail, properties of mutations D778A Q783A transmembrane segment M6 Pmr1. In phenotypic screens Ca2+ chelator Mn2+ toxicity reported separately (Wei,...
Sporadic pituitary adenomas occur in over 10% of the population. Hormone-secreting adenomas, including those causing Cushing's disease (CD), cause severe morbidity and early mortality. Mechanistic studies CD are hindered by a lack vitro models control normal human glands. Here, we surgically annotate adjacent glands 25 34 patients. Using single-cell RNA sequencing (RNA-seq) analysis 27594 cells, identify adenoma transcriptomic signatures compared with validation bulk RNA-seq, DNA...
INTRODUCTION: Spinal ependymomas often recur following surgical resection. Grade 3 tumors, particularly those harboring MYCN amplification, have dismal prognoses despite best treatment regimes. METHODS: amplification was identified with the Oncomine panel and bulk methylation analysis (MYCN). We conducted paired single-nucleus RNA (snRNA) chromatin accessibility (snATAC) sequencing (10x Genomics) of grade 2 spinal (7 patients, 1 repeat surgery). Additional snRNAseq samples (8 3) were...
INTRODUCTION: The mechanism of progression Cushing’s disease (CD) adenomas to an aggressive phenotype (Ki67 > 3%) remains unknown. Polycombs are master epigenetic regulators that catalyze H3K27 trimethylation and induce gene silencing in tumors. METHODS: We performed multiome analysis (single nucleus RNA-seq ATAC-seq, 10x Genomics) 5 CD adenomas, verified key protein pathways using mIHC. Polycomb activity was assessed H3K27me3 ChIP-seq. ATT20 cells were treated polycomb inhibitors DZNep...
A heavy metal hypertolerant plant growth-promoting bacterium was isolated from the arsenic-contaminated garden soil of Bhagobangola I block (Murshidabad district). Metagenomic classification identified bacterial isolate as a member genus Microbacterium. Taxonomic assessment strain revealed its maximum average nucleotide identity (89.99 %) with Microbacterium paraoxydans DSM 15019. Prokaryotic genome annotation performed by Prokka, DFAST and RAST. The entire consisted 3365911 bases 69.90 % GC...
PMR1 is the yeast secretory pathway pump responsible for high affinity transport of Mn2+ and Ca2+ into Golgi, where these ions are sequestered effectively removed from cytoplasm. Phenotypic growth assays allow convenient screening side chains important transport. Earlier we demonstrated that mutant Q783A at cytoplasmic interface M6 could Ca2+, but not Mn2+. Scanning mutagenesis proximal to residue Gln-783 in membrane helices M2, M4, M5, revealed additional residues near interface, notably...
The human multidrug transporter P-glycoprotein (Pgp or ABCB1) sets up pharmacological barriers to many clinically important drugs, a therapeutic remedy for which has yet be formulated. For the rational design of mechanism-based inhibitors (or modulators), it is necessary map potential sites modulator interaction and understand their modes communication with other functional domains Pgp. In this study, combining directed mutagenesis homology modeling, we provide evidence two...
Experiments from other laboratories conducted with <i>Leishmania donovani</i> promastigote cells had earlier indicated that the plasma membrane Mg<sup>2+</sup>-ATPase of parasite is an extrusion pump for H<sup>+</sup>. Taking advantage pellicular microtubular structure organism, we report procedures obtaining sealed ghost and everted vesicle defined polarity. Rapid influx H<sup>+</sup>into vesicles was found to be dependent on simultaneous presence ATP (1 mm) Mg<sup>2+</sup> mm). Excellent...
Controlled exposure of Leishmania donovanipromastigotes to hypotonic shock results in the formation deflagellated unsealed ghosts original polarity that largely retain pellicular microtubular structure associated with plasma membrane parasite. Gentle shearing followed by suspension purified appropriate isotonic buffer containing Mg2+ (4 mM) sealed everted vesicles. The presence appears be essential for efficient sealing and also prevent leakiness. ATP-dependent Ca2+ accumulation can...
The promastigote stage of Leishmania resides in the sand fly gut, enriched with sugar molecules. Recently we reported that donovani possesses a sucrose uptake system and stable pool intracellular metabolizing enzyme. In present study, purified sucrase nearly to its homogeneity compared it extracellular sucrase. estimated size is ~112 kDa by gel filtration chromatography, native PAGE, substrate staining. However, SDS-PAGE, protein resolved at ~56 kDa, indicating possibility homodimer state....
The soil and groundwater of the Bhagobangola I block Murshidabad district, West Bengal, India is severely arsenic-contaminated. A bacterium was isolated from garden Mahishasthali village, which could tolerate 36.49 mM arsenic (III), 280.44 (V) 63 chromium makes it (III V) (III) hyper-tolerant bacterium. growth pattern this does not show much alteration in presence 10 emphasizing its resistance to these heavy metals. Scanning electron microscopic analysis depicted be rod-shaped with a size...
Abstract A heavy metal hypertolerant plant growth promoting bacterium was isolated from arsenic contaminated garden soil of Bhagobangola I block (Murshidabad district). Metagenomic classification identified the bacterial isolate as a member genus Microbacterium . Taxonomic assessment showed maximum average nucleotide identity ( 89.99%) with paraoxydans strain DSM 15019. Prokaryotic genome annotation performed by Prokka, DFAST and RAST. The entire consisted 3365911 bases 69.90% GC-content....
<title>Abstract</title> <bold>Background:</bold> The protein kinase-phosphatase equilibrium is essential for eukaryotic development and homeostasis, but its epigenomic dysregulation in human tumors remains unexplored.<bold>Objectives/Methods:</bold> We employed an omics-based approach to elucidate the molecular mechanisms of pituitary adenomas, which comprise 20% primary brain tumors. created paired datasets adenomas adjacent normal glands, assaying chromatin accessibility, DNA methylation,...
Abstract INTRODUCTION Aggressive Cushing’s disease (CD) tumors (Ki67 &gt; 3%) exhibit rapid growth, poor postsurgical outcomes, and resistance to conventional therapies. Polycomb group (PcG) proteins are canonical epigenetic silencers that can drive tumor aggressiveness. We asked if PcG underlies rare but fatal pituitary progression. METHODS performed paired multiome analysis (single nucleus RNAseq ATACseq) in 5 aggressive CD adenomas, adjacent normal gland. verified protein expression...
Abstract Grade-3 spinal ependymomas, particularly those harboring MYCN amplification, have dismal prognoses despite optimal treatment regimens. functions as a transcription factor (TF) to drive the expression of key tumor driver genes. Our aim is identify mechanisms underlying aggressiveness and resistance in MYCN-amplified ependymomas. amplification ependymomas was identified with custom next generation sequencing panels bulk methylation analysis. We then conducted paired single-nucleus RNA...
Arsenic (As) contamination of groundwater is a common problem in BDP. The alkaline pH the provides favourable condition for geomicrobiochemical processes, where organic matter also plays an important role mobilization As. total arsenic content (AsT) range 69-178 µgL-1 with mean value 118.58 µgL-1. quality monitored drinking water wells are related to local land–use pattern and rural sanitation installation practices (which can have strong influence on microbial contamination). study...