A5021729725- Virus-based gene therapy research
- Monoclonal and Polyclonal Antibodies Research
- RNA Interference and Gene Delivery
- Radiopharmaceutical Chemistry and Applications
- CAR-T cell therapy research
- Viral Infectious Diseases and Gene Expression in Insects
- Glycosylation and Glycoproteins Research
- Cancer Research and Treatments
- CRISPR and Genetic Engineering
- Histone Deacetylase Inhibitors Research
- Immunotherapy and Immune Responses
- Cancer, Hypoxia, and Metabolism
- Medical Imaging and Pathology Studies
- Biochemical and Molecular Research
- HER2/EGFR in Cancer Research
- Peptidase Inhibition and Analysis
- Medical Imaging Techniques and Applications
- Cancer therapeutics and mechanisms
- Macrophage Migration Inhibitory Factor
- Ovarian cancer diagnosis and treatment
- Protein purification and stability
- Cell Adhesion Molecules Research
- Nanoparticle-Based Drug Delivery
- Neuroendocrine Tumor Research Advances
- Nuclear Receptors and Signaling
University of Groningen
2012-2024
Modulation Therapeutics (United States)
2002-2016
University Medical Center Groningen
2008-2012
University of Alabama at Birmingham
2009
University of Malawi
2005
Vrije Universiteit Amsterdam
1994-2003
Amsterdam UMC Location Vrije Universiteit Amsterdam
1999-2003
Johns Hopkins Hospital
2002
Gene Therapy Laboratory
2002
University of Amsterdam
1990-2000
Despite the rapid development of CRISPR/Cas9-mediated gene editing technology, potential CRISPR/Cas9 is hampered by low efficiency, especially for clinical applications. One major challenges that chromatin compaction inevitably limits Cas9 protein access to target DNA. However, precisely regulated histone acetylation and deacetylation. To overcome these challenges, we have comprehensively assessed impacts modifiers such as HDAC (1-9) inhibitors HAT (p300/CBP, Tip60 MOZ) inhibitors, on...
Ursodeoxycholic acid (UDCA) is used in the treatment of cholestatic liver diseases, but its mechanism action not yet well defined. The aim this study was to explore protective mechanisms taurine-conjugate UDCA (tauroursodeoxycholic [TUDCA]) against glycochenodeoxycholic (GCDCA)-induced apoptosis primary cultures rat hepatocytes. Hepatocytes were exposed GCDCA, TUDCA, glyco-conjugate (GUDCA), and TCDCA. phosphatidylinositol-3 kinase pathway (PI3K) nuclear factor-kappaB inhibited using LY...
The increasing number of patients suffering from chronic obstructive pulmonary disease (COPD) represents a major and health problem. Therefore, novel therapeutic approaches are needed. Class I HDACs 1, 2 3 play key roles in the regulation inflammatory gene expression with particular pro-inflammatory role for HDAC 3. has been reported to be an important player inflammation by deacetylating NF-κB p65, which implicated pathology COPD. Here, we applied pharmacological 3-selective inhibitor...
To assess the possibilities of retargeting adenovirus to activated endothelial cells, we conjugated bifunctional polyethylene glycol (PEG) onto adenoviral capsid inhibit interaction between viral knob and coxsackie-adenovirus receptor (CAR). Subsequently, introduced an alphav integrin-specific RGD peptide or E-selectin-specific antibody other functional group PEG molecule for cells. In vitro studies showed that this approach resulted in elimination transgene transfer into CAR-positive while...
Abstract Chronic obstructive pulmonary disease (COPD) constitutes a major health burden. Studying underlying molecular mechanisms could lead to new therapeutic targets. Macrophages are orchestrators of COPD, by releasing pro-inflammatory cytokines. This process relies on transcription factors such as NF-κB, among others. NF-κB is regulated lysine acetylation; post-translational modification installed histone acetyltransferases and removed deacetylases (HDACs). We hypothesized that small...
Adenoviruses are common pathogens associated with respiratory diseases, gastrointestinal illnesses and/or conjunctivitis. Currently, this virus is used as a vector in gene therapy trials. The promise of viral applications substantially reduced because the cleared by liver macrophages upon systemic administration. mechanism underlying adenoviral tropism to and degradation poorly understood. We identified new receptor, scavenger receptor A (SR-A), responsible for uptake macrophages. CHO cells...