A5025128636- Developmental Biology and Gene Regulation
- Hemoglobin structure and function
- Neurobiology and Insect Physiology Research
- Iron Metabolism and Disorders
- Hemoglobinopathies and Related Disorders
- Physiological and biochemical adaptations
- Cancer-related gene regulation
- Planarian Biology and Electrostimulation
- Marine Ecology and Invasive Species
- Genomics and Phylogenetic Studies
- Epigenetics and DNA Methylation
- Protist diversity and phylogeny
- Medical and Biological Sciences
- Mediterranean and Iberian flora and fauna
- RNA Research and Splicing
- Echinoderm biology and ecology
- Ectopic Pregnancy Diagnosis and Management
- Marine Invertebrate Physiology and Ecology
- Botany, Ecology, and Taxonomy Studies
- Cephalopods and Marine Biology
- Invertebrate Immune Response Mechanisms
- Homicide, Infanticide, and Child Abuse
- Grief, Bereavement, and Mental Health
- Cellular Mechanics and Interactions
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
Centre National de la Recherche Scientifique
2008-2024
Université Paris Cité
2009-2024
Institut Jacques Monod
2009-2024
Délégation Paris 7
2013-2016
Sorbonne Paris Cité
2016
Cornell University
2011
Centre de Génétique Moléculaire
2006-2009
Bajcsy-Zsilinszky Kórház és Rendelőintézet
1967
University of Washington
1967
University of Szeged
1967
To reconstruct the evolutionary origin of multicellular animals from their unicellular ancestors, genome sequences diverse relatives are essential. However, only choanoflagellate Monosiga brevicollis has been reported to date. Here we completely sequence filasterean Capsaspora owczarzaki, closest known relative metazoans besides choanoflagellates. Analyses this alter our understanding molecular complexity metazoans' ancestors showing that they had a richer repertoire proteins involved in...
Abstract Background Molecular and genetic analyses conducted in model organisms such as Drosophila vertebrates, have provided a wealth of information about how networks transcription factors control the proper development these species. Much less is known, however, evolutionary origin elaborated their large-scale evolution. Here we report first analysis whole superfamily factors, basic helix-loop-helix (bHLH) proteins, at scale metazoan kingdom. Results We identified silico putative full...
Functional studies in model organisms, such as vertebrates and Drosophila, have shown that basic Helix-loop-Helix (bHLH) proteins important roles different steps of neurogenesis, from the acquisition neural fate to differentiation into specific cell types. However, these highlighted many differences expression function orthologous bHLH during development between Drosophila. To understand how functions genes evolved among bilaterians, we performed a detailed study nervous system polychaete...
RNA-binding proteins (RBPs) are key players in various biological processes, most notably regulation of gene expression at the posttranscriptional level. Although many RBPs have been carefully studied model organisms, very few studies addressed evolution these scale animal kingdom. We identified a large set putative encoded by genome demosponge Amphimedon queenslandica, species representing basal lineage. compared with those genomes two bilaterians (human and Drosophila), representatives...
ABSTRACT Notch signaling is an evolutionarily conserved pathway known to orchestrate neurogenesis by regulating the transition of progenitor cells differentiated neurons and glia, as well directing neurite outgrowth axon guidance in many species. Although extensively studied vertebrates ecdysozoans, role spiralians (including mollusks, annelids or flatworms) remains largely unexplored, limiting our understanding its functions across bilaterians. In this study, we focus on segmented annelid...
The iroquois (iro/Irx) genes encode transcriptional regulators that belong to the TALE superclass of homeodomain proteins and have key functions during development in both vertebrates insects. Irx occur one or two genomic clusters containing three each within Drosophila several vertebrate genomes, respectively. similar organization is widely considered as a result convergent evolution, due independent tandem gene duplications. In this study, we investigate evolutionary history at scale whole...
Abstract The notochord is the distinctive characteristic of chordates; however, knowledge complement transcription factors governing development this structure still incomplete. Here we present expression patterns seven factor genes detected in ascidian Ciona intestinalis at various stages embryonic development. Four these factors, Fos‐a, NFAT5, AFF and Klf15 , have not been directly associated with previous studies, while others, including Spalt‐like‐a, Lmx‐like STAT5/6‐b display...
Prdm genes encode transcription factors with a subtype of SET domain known as the PRDF1-RIZ (PR) homology and variable number zinc finger motifs. These are involved in wide variety functions during animal development. As most have been studied vertebrates, especially mice, little is about evolution this gene family. We searched for fully sequenced genomes 93 different species representative all main metazoan lineages. A total 976 were identified these species. The per ranges from 2 to 19. To...
Methylation of cytosines in DNA (5mC methylation) is a major epigenetic modification that modulates gene expression and constitutes the basis for mechanisms regulating multiple aspects embryonic development cell reprogramming vertebrates. In mammals, 5mC methylation promoter regions linked to transcriptional repression. Transcription regulation by notably involves nucleosome remodeling deacetylase complex (NuRD complex) which bridges histone modifications. However, less known about...
SUMMARY The molecular mechanisms underlying the formation and patterning of nervous system are relatively poorly understood for lophotrochozoans (like annelids) as compared with ecdysozoans (especially Drosophila ) deuterostomes vertebrates). Therefore, we have undertaken a candidate gene approach to study aspects neurogenesis in polychaete annelid Platynereis dumerilii . We determined spatiotemporal expression orthologs four genes ( SoxB, Churchill, prospero / Prox , SoxC) known play key...
The notochord is a defining feature of the chordate clade, and invertebrate chordates, such as tunicates, are uniquely suited for studies this structure. Here we used well-characterized set 50 genes known to be targets notochord-specific Brachyury transcription factor in one tunicate, Ciona intestinalis (Class Ascidiacea), begin determining whether same genetic toolkit employed build another Oikopleura dioica Larvacea). We identified orthologs genes, well lineage-specific duplicates which...
Segmented body organizations are widely represented in the animal kingdom. Whether last common bilaterian ancestor was already segmented is intensely debated. Annelids display broad morphological diversity but many species among most homonomous metameric animals. The front end (prostomium) and tail piece (pygidium) of annelids classically described as non-segmental. However, pygidium structure development remain poorly studied.
Data from 354 embryos and fetuses between 20 200 mm crown rump length obtained by therapeutic abortion in 3 different countries were evaluated. All Danich Hungarian the majority of American specimens measured immeditely after delivery fresh condition. In mathematical evaluation linear regressions calculated method least squares for arbitrarily defined ranges to 20-50 50-200 lenghts. The material was analyzed statistically so that confidence limits could be drawn estimation gestational age...
How vascular systems and their respiratory pigments evolved is still debated. While many animals present a system, hemoglobin exists as blood pigment only in few groups (vertebrates, annelids, arthropod mollusk species). Hemoglobins are formed of globin sub-units, belonging to multigene families, various multimeric assemblages. It was so far unclear whether families from different bilaterian had common origin.
Genes of the coe (collier/olfactory/early B-cell factor) family encode Helix-Loop-Helix transcription factors that are widely conserved in metazoans and involved many developmental processes, neurogenesis particular. Whereas their functions during vertebrate neural tube formation have been well documented, very little is known about expression role central nervous system (CNS) development protostomes. Here we characterized CNS genes insect Drosophila melanogaster polychaete annelid...
Abstract Background An important issue concerning the evolution of duplicated genes is to understand why paralogous are retained in a genome even though most likely fate for redundant gene nonfunctionalization and thereby its elimination. Here we study complex superfamily generated by duplications, snail related that play key roles during animal development. We investigate evolutionary history these genomic, phylogenetic, expression data studies. Results systematically retrieved full...
Dbx1 is a homeodomain transcription factor involved in neuronal fate specification belonging to widely conserved family among bilaterians. In mammals, was proposed act as transcriptional repressor by interacting with the Groucho corepressors allow of neurons essential biological functions such locomotion or breathing.Sequence alignments proteins from different species allowed us identify two domains related Groucho-dependent Engrailed domain (RD), well newly described composed clusterized...
ABSTRACT Background Methylation of cytosines in DNA (5mC methylation) is a major epigenetic modification that modulates gene expression and important for embryonic development cell reprogramming vertebrates. In mammals, 5mC methylation promoter regions linked to transcriptional repression. Transcription regulation by notably involves the Nucleosome Remodeling Deacetylase complex (NuRD complex) which bridges histone modifications. Less known about roles mechanisms non-vertebrate animals. this...
Mid-tailbud Ciona intestinalis embryo expressing GFP driven by the notochord specific cis-regulatory module associated with transcription factor Brachyury (green cells). Embryos are counterstained phalloidin (red) and DAPI (blue) to highlight F-actin nuclei, respectively. From José-Edwards et al., Developmental Dynamics 240:1793–1805, 2011.