A5023456573- Plant-Microbe Interactions and Immunity
- Microplastics and Plastic Pollution
- Microbial bioremediation and biosurfactants
- Legume Nitrogen Fixing Symbiosis
- biodegradable polymer synthesis and properties
- Biofuel production and bioconversion
- bioluminescence and chemiluminescence research
- Microbial Community Ecology and Physiology
- 3D Printing in Biomedical Research
- Genomics and Phylogenetic Studies
- Nematode management and characterization studies
- Soil Carbon and Nitrogen Dynamics
- Phosphorus and nutrient management
- Plant tissue culture and regeneration
- Wastewater Treatment and Nitrogen Removal
- Recycling and Waste Management Techniques
- Mycorrhizal Fungi and Plant Interactions
- Electrokinetic Soil Remediation Techniques
- Nanocomposite Films for Food Packaging
- Plant Disease Resistance and Genetics
- Nitrogen and Sulfur Effects on Brassica
- Genetic diversity and population structure
- Anaerobic Digestion and Biogas Production
- Advances in Cucurbitaceae Research
- Soil and Water Nutrient Dynamics
Institute of Technology Carlow
2012-2024
South East Technological University
2022-2024
Technological University Dublin
2023
The use of plant growth promoting bacterial inoculants as live microbial biofertilizers provides a promising alternative to chemical fertilizers and pesticides. Inorganic phosphate solubilization is one the major mechanisms promotion by associated bacteria. This involves bacteria releasing organic acids into soil which solubilize complexes converting them ortho-phosphate available for up-take utilization. study presented here describes ability endophytic produce gluconic acid (GA), insoluble...
2,4-Dichlorophenoxyacetic acid is a selective systemic herbicide for the control of broad-leaved weeds, which widely used throughout world. The persistence its residues and potential to migrate in soil make it necessary reduce concentrations contaminated groundwater. nature this compound makes particularly toxic plants, such as poplar (Populus) willow (Salix), are often phytoremediation projects. We describe inoculation model plant, pea (Pisum sativum), with genetically tagged bacterial...
With the exception of nitrogen fixing bacteria, there is little known about colonisation patterns or population sizes bacterial endophytes in deciduous trees. This study describes isolation, identification, construction and re-colonisation three green fluorescent protein(gfp):kanamycin(R) labelled when re-introduced into poplar trees, their original host plant. Two these showed considerable roots stems inoculated plants. gfp expressing cells all strains were observed to colonise xylem tissue...
Polyaromatic hydrocarbons (PAHs) are major and recalcitrant pollutants of the environment their removal presents a significant problem. Phytoremediation has shown much promise in PAH from contaminated soil, but may be inhibited because plant experiences phytotoxic effects low-molecular-weight PAHs such as naphthalene. This paper describes construction naphthalene-degrading endophytic strain designated Pseudomonas putida VM1441(pNAH7). was found to an efficient colonizer plants, colonizing...
Plant associated bacteria with Growth Promotion (PGP) properties have been proposed for use as environmentally friendly biofertilisers sustainable agriculture, however, analysis of their efficacy in the field is often limited. In this study, greenhouse and trials were carried out using individual endophytic Pseudomonas fluorescens strains, well characterised rhizospheric P. F113 an microbial consortium 10 different strains. These had previously respect to PGP vitro shown harbor a range...
Pseudomonas fluorescens F113 is a plant growth-promoting rhizobacterium (PGPR) isolated from the sugar-beet rhizosphere. This bacterium has been extensively studied as model strain for genetic regulation of secondary metabolite production in P. fluorescens, candidate biocontrol agent against phytopathogens, and heterologous host expression genes with biotechnological application. The genome sequence annotation recently reported.Comparative analysis 50 sequences strains belonging to group...
Pseudomonas fluorescens F113 is a plant growth-promoting rhizobacterium (PGPR) that has biocontrol activity against fungal pathogens and model for rhizosphere colonization. Here, we present its complete genome sequence, which shows besides core very similar to those of other strains sequenced within this species, possesses wide array genes encoding specialized functions thriving in the interacting with eukaryotic organisms.
The soil microbiome consists of a vast variety microorganisms which contribute to essential ecosystem services including nutrient recycling, protecting structure, and pathogen suppression. Recalcitrant organic compounds present in soils contaminated with fuel oil can lead decrease functional redundancy within microbiomes. Ecopiling is passive bioremediation technique involving biostimulation indigenous hydrocarbon degraders, bioaugmentation through inoculation known petroleum-degrading...
Microplastics (MPs) are environmental pollutants of growing concern, and awareness MPs pollution in marine freshwater environments has increased recent years. However, knowledge contamination riverine sediments Ireland is limited. To address this, we collected analysed sediment samples from 16 selected sites along the River Barrow. were extracted through a density separation method, after which their size, colour, shape under stereo microscope (Optica SZM-2). Attenuated total reflection...
Biopiling is an ex situ bioremediation technology that has been extensively used for remediating a wide range of petrochemical contaminants in soils. involves the assembling contaminated soils into piles and stimulating biodegrading activity microbial populations by creating near optimum growth conditions. Phytoremediation another very successful technique use plants their associated microbiomes to degrade, sequester or bio-accumulate pollutants from soil water. The objective this study was...
Pseudomonas fluorescens F113Rifpcb is a genetically engineered rhizosphere bacterium with the potential to degrade polychlorinated biphenyls (PCBs). F113Rifpcbgfp and F113L::1180gfp are biosensor strains capable of detecting PCB bioavailability biodegradation. The aim this paper evaluate use alginate beads as storage, delivery containment system for these in contaminated soils.The survival release Ps. from were evaluated. Two F113-based encapsulated, their ability detect 3-chlorobenzoate...
Gaining a greater understanding of the plant microbiota and its interactions with host heralds new era scientific discovery in agriculture. Different agricultural management practices influence soil microbial populations by changing soil's physical, chemical biological properties. However, impact these on associated economically important crops such as oilseed rape, are still understudied. In this work we investigated two contrasting crop establishment practices, conventional (plough based)...
Whole cell microbial biosensors offer excellent possibilities for assaying the complex nature of bioavailable and bioaccessible fraction pollutants in contaminated soils, which currently cannot be easily addressed. This paper describes application evaluation three biosensor strains designed to detect bioavailability biodegradation PCBs (and end-products) soils sediments. Polychlorinated biphenyls (PCBs) are considered one most wide spread, hazardous persistent pollutants. Herein we describe...
This study aimed at assessing the morphological variation in 25 accessions of an Irish collection Brassica oleracea using 44 traits. The traits showed a pronounced among accessions. Vegetative preflowering and maturity revealed higher than seedling Of descriptors used, 28 were significantly different proved useful examining diversity differentiating studied. Principal component analysis (PCA) accessions, where 87.24% total data was separated on first five components, indicating high degree...
Environmentally friendly packaging is becoming more popular as the number of companies implementing sustainable solutions continues to increase, and consumers become aware choose environmentally options. However, not all meets desirable properties, some are only partially made renewable raw materials or degrade over a long period time. Bioplastics constructed from blends gelatine starch solely materials. Combined with relatively short degradation times, these have potential replace currently...
Phosphorus (P) is an essential plant nutrient routinely applied to soils as agricultural fertiliser, frequently in non-renewable, inorganic forms. Finite reserves and growing demand for phosphorus mean alternative P resources need be explored. Recycling-derived fertilisers (RDF) recovered from specific waste streams, using recovery technologies, have the potential replace conventional used agriculture. Healthy functioning soil microbial nematode communities are players maintaining health...
Ecopiling is a method for biodegradation of hydrocarbons in soils. It derives from Biopiles, but phytoremediation added to biostimulation with nitrogen fertilization and bioaugmentation local bacteria. We have constructed seven Ecopiles soil heavily polluted Carlow (Ireland). The aim the study was analyze changes microbial community during ecopiling. In course 18 months remediation, total petroleum values decreased 99 88% on average aliphatics aromatics, respectively, indicating successful...
Green energy and biofuel policy is promoting the replacement of a significant percentage fossil fuel resources with renewable bioenergy carriers to meet future requirements reduce carbon emissions. However, global rise in nitrogen pollution, due increased food crop production, has raised environmental concerns. Strategies for managing sustainable production while maximizing benefits should be an important milestone all biofuel-producing countries. In this article we review recent literature...
Possible amendments to the European Commission's Nitrates Directive, such as proposal of 'RENURE' criteria for use mineral nitrogen (N) bio-based fertilisers (BBFs) a substitute Haber-Bosch derived chemical N fertilisers, is expected open novel avenues BBF in near future. Short and long-term testing RENURE materials field trials will provide comprehensive insights into their crop response environmental impacts. In this study, three potential (ammonium nitrate (AN) from stripping/scrubbing,...