A5101884769- DNA Repair Mechanisms
- Bacterial Genetics and Biotechnology
- DNA and Nucleic Acid Chemistry
- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- Genomics and Chromatin Dynamics
- RNA and protein synthesis mechanisms
- Microtubule and mitosis dynamics
- Bacteriophages and microbial interactions
- Retinal Development and Disorders
- Nerve injury and regeneration
- Epigenetics and DNA Methylation
- CRISPR and Genetic Engineering
- Metabolism and Genetic Disorders
- Cleft Lip and Palate Research
- Chromosomal and Genetic Variations
- Axon Guidance and Neuronal Signaling
- Prion Diseases and Protein Misfolding
- Endoplasmic Reticulum Stress and Disease
- Amino Acid Enzymes and Metabolism
- Pluripotent Stem Cells Research
- Animal Genetics and Reproduction
- Photoreceptor and optogenetics research
- Escherichia coli research studies
- Nuclear Receptors and Signaling
National Center For Child Health and Development
2015-2024
Tokyo Metropolitan Institute of Medical Science
2002-2020
Chiba Institute of Technology
2013
Japan Science and Technology Agency
2013
Tokyo University of Science
1999-2013
Saitama Cancer Center
2013
Hokkaido University
2010
Kanagawa Prefectural Hospital Organization
2009
Kanagawa Cancer Center
2009
National Institute of Advanced Industrial Science and Technology
2006
Cdc7 kinase, conserved from yeasts to human, plays important roles in DNA replication. However, the mechanisms by which it stimulates initiation of replication remain largely unclear. We have analyzed phosphorylation MCM subunits during cell cycle examining mobility shift on SDS-PAGE. MCM4 chromatin undergoes specific S phase. phosphorylates complexes as well N-terminal polypeptide. Experiments with phospho-amino acid-specific antibodies indicate that phase-specific is due at (S/T)(S/T)P...
We generated self-induced retinal ganglion cells (RGCs) with functional axons from human induced pluripotent stem cells. After development of the optic vesicle cell embryoid body in three-dimensional culture, conversion to two-dimensional achieved by supplementation BDNF, resulted differentiation RGCs at a rate nearly 90% as indicated marginal subregion an extruded clump cells, suggesting formation vesicle. Axons extended radially margin clump. Induced expressed specific markers, such Brn3b...
PriA helicase plays crucial roles in restoration of arrested replication forks. It carries a "3' terminus binding pocket" its N-terminal DNA domain, which is required for high affinity to fork carrying 3'-end nascent leading strand at the branch. We show that abrogation 3' recognition either by mutation pocket or bulky modification leads unwinding unreplicated duplex arm on this fork, causing potential destabilization. This indicates critical role "stable" primosome assembly. In contrast,...
Escherichia coli PriA protein plays crucial roles in processing of arrested replication forks. serves as a sensor/stabilizer for an fork and eventually promotes restart DNA through assembly primosome. carries 3' terminus binding pocket required its high affinity to specific well biological functions. We show here that binds manner either dependent on or independent recognition. The former mode requires the present at N-terminal half 181-residue domain exhibits bipartite interaction template...
Arrest of replication forks by various internal and external threats evokes a myriad cellular reactions, collectively known as DNA checkpoint responses. In bacteria, PriA is essential for restoration stalled recombinational repair double-stranded breaks candidate sensor protein that may recognize arrested forks. Here, we report specifically recognizes 3′ termini nascent chains at model in vitro. Mutations the putative "3′ terminus binding pocket" present N-terminal segment result reduced to...
AML1 (RUNX1) is a key transcription factor for hematopoiesis that binds to the Runt-binding double-stranded DNA element (RDE) of target genes through its N-terminal Runt domain. Aberrations in gene are frequently found human leukemia. To better understand and potential utility diagnosis therapy, we obtained RNA aptamers bind specifically Enzymatic probing NMR analyses revealed Apt1-S, which truncated variant one aptamers, has CACG tetraloop two stem regions separated by an internal loop. All...
PriA protein is essential for RecA-dependent DNA replication induced by stalled forks in <i>Escherichia coli</i>. a DE<i>X</i>H-type helicase, ATPase activity of which depends on its binding to structured including D-loop-like structure. Here, we show that the N-terminal 181-amino acid polypeptide can form complex with D-loop gel shift assays and have identified unique motif present segment plays role binding. We also residues C terminus proximal helicase domain proteins mutated this do not...
Abstract Backgrounds: PriA protein, a DEXH‐type helicase with C 2 zinc‐finger motifs, plays essential roles in RecA‐dependent modes of Escherichia coli chromosomal DNA replication, namely inducible and constitutive stable replication (iSDR cSDR respectively, which may be initiated from D‐loop or R‐loop structure), repair double‐stranded breaks generated by various genotoxic agents spontaneously during the course replication. However, ATPase/DNA activities functions are not well understood....
The identification of genes targeted by a specific transcription regulatory factor (TRF) is essential to our understanding the mechanism gene expression. We constructed system for comprehensive directly regulated TRF. It includes combination perturbation expression RNA interference (RNAi) TRF, cDNA microarray analysis, computer searches putative TRF recognition sequences, and in vivo vitro TRF–DNA binding assays. Endogenous hepatocyte nuclear factor‐1β (HNF‐1β) mRNA was efficiently degraded...
Replication fork protection complex Swi1-Swi3 and replication checkpoint mediator Mrc1 are required for maintenance of integrity during the course DNA in fission yeast Schizosaccharomyces pombe. These proteins play crucial roles stabilizing stalled forks activating signaling pathways. Although they conserved components, precise biochemical these not known. Here we purified show that bind to independently but synergistically vitro. binds preferentially arrested or D-loop-like structures,...
AML1/RUNX1 is an essential transcription factor involved in the differentiation of hematopoietic cells. AML1 binds to Runt-binding double-stranded DNA element (RDE) target genes through its N-terminal Runt domain. In a previous study, we obtained RNA aptamers against domain by systematic evolution ligands exponential enrichment and revealed that exhibit higher affinity for than RDE possess 5′-GCGMGNN-3′ 5′-N′N′CCAC-3′ conserved motif (M: A or C; N N′ form Watson–Crick base pairs) important...
Retinal ganglion cells (RGCs) are specialized projection neurons that constitute part of the retina, and death RGCs causes various eye diseases, but mechanism RGC is still unclear. Here, we induced cell in human pluripotent stem (hiPSC)-derived RGC-rich retinal tissues using hypoxia–reoxygenation vitro. Flow cytometry, immunochemistry, Western blotting showed apoptosis necrosis under hypoxia–reoxygenation, they were rescued by an inhibitor not a inhibitor. This revealed our model was mainly...
We recently established a novel method for generating functional human retinal ganglion cells (RGCs) from induced pluripotent (hiPSCs). Here, we confirmed that RGCs can also be generated embryonic stem (hESCs). investigated the usefulness of with long axons assessing effects chemical agents, such as neurotrophic factor, nerve growth factor (NGF), and chemorepellent factors, semaphorin 3 A (SEMA3A) SLIT1. The direct local administration each agent on axonal projection were evaluated by...