A5043928379- Air Quality and Health Impacts
- Bacterial Genetics and Biotechnology
- Vibrio bacteria research studies
- Air Quality Monitoring and Forecasting
- Atmospheric chemistry and aerosols
- Protein Tyrosine Phosphatases
- Supramolecular Chemistry and Complexes
- Antibiotic Resistance in Bacteria
- RNA and protein synthesis mechanisms
- Escherichia coli research studies
- Crystallography and molecular interactions
- Genomics and Phylogenetic Studies
- Mollusks and Parasites Studies
- Bacterial Identification and Susceptibility Testing
- Impact of Light on Environment and Health
- Bacteriophages and microbial interactions
- Porphyrin and Phthalocyanine Chemistry
- Structural and Chemical Analysis of Organic and Inorganic Compounds
- Synthetic Organic Chemistry Methods
- Flood Risk Assessment and Management
- Cancer-related Molecular Pathways
- Chemical Synthesis and Characterization
- Protein Kinase Regulation and GTPase Signaling
- Rabies epidemiology and control
- Heavy metals in environment
University of Dundee
2006-2025
Wellcome Sanger Institute
2022-2025
University of York
2016-2018
Oklahoma State University
2009
Oklahoma State University Oklahoma City
2006
University of Southern Denmark
2006
Ninewells Hospital
2000-2005
Cancer Research UK
2002-2005
Imperial College London
1997-2004
University of California, Los Angeles
2000
Synthetic approaches to self-assembling [n]rotaxanes incorporating π-electron deficient bipyridinium-based dumbbell-shaped components and rich hydroquinone-based macrocycles have been developed. In particular, the so-called slippage methodology relies upon size complementarity of preformed macrocyclic components. The spontaneous self-assembly these complementary into a rotaxane in solution can be achieved under influence an appropriate amount thermal energy. absorption spectra, luminescence...
The genus Serratia has been studied for over a century and includes clinically-important diverse environmental members. Despite this, there is paucity of genomic information across the robust whole genome-based phylogenetic framework lacking. Here, we have assembled analysed representative set 664 genomes from genus, including 215 historic isolates originally used in defining genus. Phylogenomic analysis reveals clearly-defined population structure which displays deep divisions aligns with...
Abstract Photochemical control of a self‐assembled supramolecular 1:1 pseudorotaxane (formed between tetracationic cyclophane, namely the tetrachloride salt cyclobis(paraquat‐ p ‐phenylene), and 1,5‐bis[2‐(2‐(2‐hydroxy)ethoxy)ethoxy]naphthalene) has been achieved in aqueous solution. The photochemical one‐electron reduction cyclophane to radical trication weakens noncovalent bonding interactions naphthalene guest—π‐π π‐electron‐rich π‐electron‐poor aromatic systems, hydrogen‐bonding acidic...
Type VI secretion systems (T6SSs) are nanomachines widely used by bacteria to deliver toxic effector proteins directly into neighbouring cells. However, the modes of action many effectors remain unknown. Here we report that Ssp6, an anti-bacterial delivered a T6SS opportunistic pathogen Serratia marcescens, is toxin forms ion-selective pores. Ssp6 inhibits bacterial growth causing depolarisation inner membrane in intoxicated cells, together with increased outer permeability. Reconstruction...
Abstract Many bacterial species use Type VI secretion systems (T6SSs) to deliver anti-bacterial effector proteins into neighbouring cells, representing an important mechanism of inter-bacterial competition. Specific immunity protect bacteria from the toxic action their own effectors, whilst orphan without a cognate may provide protection against incoming effectors non-self competitors. T6SS-dependent Rhs contain variable C-terminal toxin domain (CT), with protein encoded immediately...
To elucidate the physiological role(s) of DUSP9 (dual-specificity phosphatase 9), also known as MKP-4 (mitogen-activated protein kinase [MAPK] 4), gene was deleted in mice. Crossing male chimeras with wild-type females resulted heterozygous (DUSP9+/−) females. However, when these animals were crossed (DUSP9+/y) males none progeny carried targeted allele, indicating that both female and null (DUSP9−/y) die utero. The is on X chromosome, this pattern embryonic lethality consistent selective...
Abstract Gram-negative bacteria depend on energised protein complexes that connect the two membranes of cell envelope. However, β-barrel outer-membrane proteins (OMPs) and α-helical inner-membrane (IMPs) display quite different organisation. OMPs cluster into islands restrict their lateral mobility, while IMPs generally diffuse throughout cell. Here, using live imaging Escherichia coli , we demonstrate when transient, energy-dependent transmembrane connections are formed, become subjugated...
ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTA Supramolecular Analog of the Photosynthetic Special PairMartinus C. Feiters, Matthew T. Fyfe, M.-Victoria Martínez-Díaz, Stephan Menzer, Roeland J. M. Nolte, Fraser Stoddart, Paul van Kan, and David WilliamsView Author Information NSR Center, University Nijmegen Toernooiveld, 6525 ED Nijmegen, The Netherlands School Chemistry, Birmingham Edgbaston, B15 2TT, U.K. Chemical Crystallography Laboratory Department Imperial College South...
Protein complexes are dynamic entities; identification and quantitation of their components is critical in elucidating functional roles under specific cellular conditions. We report the first quantitative proteomic analysis human cap-binding protein complex. Components proteins associated with translation initiation eIF4F complex that may affect formation were identified quantitated distinct growth Site-specific phosphorylation eIF4E eIF4G elevated levels eIF4G:eIF4E phorbol ester treated...
Mitogen-activated protein kinases (MAPKs) play a vital role in cellular growth control, but far less is known about these signalling pathways the context of embryonic development. Duration and magnitude MAPK activation are crucial factors cell fate decisions, reflect balance between activities upstream activators specific phosphatases (MKPs). Here, we report isolation characterization murine Pyst3 gene, which encodes cytosolic dual-specificity MKP. This enzyme selectively interacts with,...
[formula: see text] A tritopic receptor, in which three benzo[24]crown-8 macrorings are fused onto a triphenylene core, forms very strong supramolecular bundle-like complexes CDCl3/CD3CN solution with trifurcated trications wherein dibenzylammonium ions linked to central benzenoid core. The triply threaded superbundles, one of has been characterized the solid state by X-ray crystallography, dissociate completely via doubly and singly intermediates when CD3SOCD3 is added progressively solution.
ABSTRACT The use of bacterial transposon mutant libraries in phenotypic screens is a well-established technique for determining which genes are essential or advantageous growth conditions interest. Standard, inactivating, cannot give direct information about whose over-expression gives selective advantage. We report the development system wherein outward-oriented promoters included mini-transposons, generation Escherichia coli and Pseudomonas aeruginosa their to probe important under...
Abstract The National Ambient Air Quality Standard (NAAQS) for ground-level ozone, previously set as an 8-hr average of 0.08 parts per million (ppm), has been revised by the U.S. Environmental Protection Agency (EPA). Historically, background concentrations originating from non-local sources were not considered to be a major constituent ozone. However, previous research shown that ozone often exceed new NAAQS 0.075 ppm, and high are particularly troublesome in mid-latitudes. This study...
Amino derivatives 3 of the trithiadiazepine ring are moderately stable crystalline solids, readily prepared from 6-bromotrithiadiazepine 1 and ammonia or amines under remarkably mild conditions; unusually for aromatic amines, amino groups tetrahedral not conjugated with, but orthogonal to, 10 π heteroaromatic rings, all which form infinite overlapping stacks in crystals.
Abstract The syntheses, characterization and coordination chemistry of two new 1,10‐phenanthroline‐based ligands are presented here. Crystallographic studies have shown that both molecules display hydrogen‐bonding π−π stacking interactions, generating interesting supramolecular assemblies in the solid state. reactions these with [RuCl 2 (PPh 3 ) ] been performed ruthenium‐amidato complexes prepared. In cases, presence a pyridyl group ligand backbone favours deprotonation one carboxyamido...
Abstract Phaseolus vulgaris L. cv. Contender (green bean) treated with sea salt aerosols, similar to ambient seashore conditions displayed foliar injury from exposures of 8, 168, and 480 hr at sedimentation rates 27, 5.7 1.5 μ g/m 2 /sec, respectively. Sodium chloride uptake was linear time. Toxicity symptoms corresponded a critical leaf tissue level 2.6% Cl regardless exposure time or rate.
The preparation and acid catalysed hydrolysis of a number isoxazolinyldioxepins is reported. Individual diastereoisomer pairs were isolated by column chromatography shown to differ significantly in their hydrolytic stability at acidic pH. crystal structures one pair reveal that conformational differences induce selective stereoelectronic effect the acetal centre more hydrolytically labile isomer, while less isomer shows no such selectivity.
The ability to compete with diverse competitors is essential for bacteria succeed in microbial communities. A widespread strategy inter-bacterial competition the delivery of antibacterial toxins, or effector proteins, directly into rival cells using Type VI secretion system (T6SS). Whilst a large number broad-spectrum enzymatic T6SS effectors have been described, relatively few which form pores target cell membranes reported. Here, we describe widely-occurring new family T6SS-dependent...