A5053750268- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- DNA Repair Mechanisms
- CRISPR and Genetic Engineering
- Mycobacterium research and diagnosis
- MicroRNA in disease regulation
- Ferroptosis and cancer prognosis
- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- Bacteriophages and microbial interactions
- Bacterial Genetics and Biotechnology
- Advanced Sensor and Energy Harvesting Materials
- Chromosomal and Genetic Variations
- Photosynthetic Processes and Mechanisms
- Legume Nitrogen Fixing Symbiosis
- Advanced biosensing and bioanalysis techniques
- Bone Tissue Engineering Materials
- Supramolecular Self-Assembly in Materials
- RNA Interference and Gene Delivery
- Electrospun Nanofibers in Biomedical Applications
- DNA and Nucleic Acid Chemistry
Consejo Superior de Investigaciones Científicas
2021-2024
Centro Nacional de Biotecnología
2021-2024
National Research Council
2024
Institut de Microelectrònica de Barcelona
2019
Electrospinning and plasma post-modification lead to PVDF scaffolds with favorable electromechanical properties enhanced wettability.
Faithful segregation of bacterial chromosomes relies on the ParABS partitioning system and SMC complex. In this work, we used single-molecule techniques to investigate role cytidine triphosphate (CTP) binding hydrolysis in critical interaction between centromere-like parS DNA sequences ParB CTPase. Using a combined optical tweezers confocal microscope, observe specific with directly. Binding around is enhanced by presence CTP or non-hydrolysable analogue CTPγS. However, proteins are also...
Long noncoding RNAs (lncRNA) are emerging as key players in cancer parts of poorly understood molecular mechanisms. Here, we investigated lncRNAs that play a role hepatocellular carcinoma (HCC) and identified NIHCOLE, novel lncRNA induced HCC with oncogenic potential the ligation efficiency DNA double-stranded breaks (DSB). NIHCOLE expression was associated poor prognosis survival patients. Depletion from cells led to impaired proliferation increased apoptosis. deficiency accumulation damage...
The synapsis of DNA ends is a critical step for the repair double-strand breaks by non-homologous end joining (NHEJ). This performed multicomponent protein complex assembled around Ku70-Ku80 heterodimers and regulated accessory factors, including long non-coding RNAs, through poorly understood mechanisms. Here, we use magnetic tweezers to investigate contributions core NHEJ proteins APLF lncRNA NIHCOLE synapsis. stabilizes bridging and, together with Ku70-Ku80, establishes minimal that...
Abstract The human DNA repair factor CtIP helps to initiate the resection of double-stranded breaks for by homologous recombination, in part through its ability bind and bridge molecules. However, is a natively disordered protein that bears no apparent similarity other DNA-binding proteins so structural basis these activities remains unclear. In this work, we have used bulk binding, single molecule tracking, bridging assays study wild-type variant better define binding domains effects...
SUMMARY Faithful segregation of bacterial chromosomes relies on the ParABS partitioning system and SMC complex. In this work, we used single molecule techniques to investigate role cytidine triphosphate (CTP) binding hydrolysis in critical interaction between centromere-like parS DNA sequences ParB CTPase. Using a combined dual optical tweezers confocal microscope, observe specific with directly. Binding around is enhanced 4-fold by presence CTP or non-hydrolysable analogue CTPγS. However,...
<div>Abstract<p>Long noncoding RNAs (lncRNA) are emerging as key players in cancer parts of poorly understood molecular mechanisms. Here, we investigated lncRNAs that play a role hepatocellular carcinoma (HCC) and identified NIHCOLE, novel lncRNA induced HCC with oncogenic potential the ligation efficiency DNA double-stranded breaks (DSB). NIHCOLE expression was associated poor prognosis survival patients. Depletion from cells led to impaired proliferation increased apoptosis....
<p>Supplementary Figures S1-S7</p>
<p>Supplementary Materials and Methods</p>
<p>Supplementary Materials and Methods</p>
<p>Supplementary Figures S1-S7</p>
Abstract The human DNA repair factor CtIP helps to initiate the resection of double-stranded breaks for by homologous recombination, in part through its ability bind and bridge molecules. However, is a natively disordered protein that bears no apparent similarity other DNA-binding proteins so structural basis these activities remains unclear. In this work, we have used bulk binding, single molecule tracking, bridging assays study wild-type variant better define binding domains effects...
<div>Abstract<p>Long noncoding RNAs (lncRNA) are emerging as key players in cancer parts of poorly understood molecular mechanisms. Here, we investigated lncRNAs that play a role hepatocellular carcinoma (HCC) and identified NIHCOLE, novel lncRNA induced HCC with oncogenic potential the ligation efficiency DNA double-stranded breaks (DSB). NIHCOLE expression was associated poor prognosis survival patients. Depletion from cells led to impaired proliferation increased apoptosis....