A5030692178- RNA modifications and cancer
- DNA Repair Mechanisms
- Cancer-related molecular mechanisms research
- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- Escherichia coli research studies
- Antibiotic Resistance in Bacteria
- Microtubule and mitosis dynamics
- RNA Research and Splicing
- Mycobacterium research and diagnosis
- Enzyme Structure and Function
- Cancer-related Molecular Pathways
- Ferroptosis and cancer prognosis
- Tuberculosis Research and Epidemiology
- CRISPR and Genetic Engineering
- Signaling Pathways in Disease
- Steroid Chemistry and Biochemistry
- Advanced Electron Microscopy Techniques and Applications
- Heat shock proteins research
- RNA Interference and Gene Delivery
- Galectins and Cancer Biology
- Complement system in diseases
- Redox biology and oxidative stress
- Cellular Mechanics and Interactions
- interferon and immune responses
Centro de Investigación del Cáncer
2019-2023
Spanish National Cancer Research Centre
2019-2023
Centro de Investigaciones Biológicas Margarita Salas
2003-2017
Consejo Superior de Investigaciones Científicas
2003-2017
University College London
2013-2014
Institute of Structural and Molecular Biology
2014
Birkbeck, University of London
2013
Institute of Cancer Research
2011
Cancer Research UK
2011
The mannose receptor family comprises four members in mammals, Endo180 (CD280), DEC-205 (CD205), phospholipase A(2) (PLA(2)R) and the (MR, CD206), whose extracellular portion contains a similar domain arrangement: an N-terminal cysteine-rich (CysR) followed by single fibronectin type II (FNII) 8-10 C-type lectin-like domains (CTLDs). These proteins mediate diverse functions ranging from matrix turnover through collagen uptake to homeostasis immunity based on sugar recognition. MR are...
The R2TP complex, comprising the Rvb1p-Rvb2p AAA-ATPases, Tah1p, and Pih1p in yeast, is a specialized Hsp90 co-chaperone required for assembly maturation of multi-subunit complexes. These include small nucleolar ribonucleoproteins, RNA polymerase II, complexes containing phosphatidylinositol-3-kinase-like kinases. structure stoichiometry yeast how it couples to are currently unknown. Here, we determine 3D organization using sedimentation velocity analysis cryo-electron microscopy. 359-kDa...
Long noncoding RNAs (lncRNA) are emerging as key players in cancer parts of poorly understood molecular mechanisms. Here, we investigated lncRNAs that play a role hepatocellular carcinoma (HCC) and identified NIHCOLE, novel lncRNA induced HCC with oncogenic potential the ligation efficiency DNA double-stranded breaks (DSB). NIHCOLE expression was associated poor prognosis survival patients. Depletion from cells led to impaired proliferation increased apoptosis. deficiency accumulation damage...
The synapsis of DNA ends is a critical step for the repair double-strand breaks by non-homologous end joining (NHEJ). This performed multicomponent protein complex assembled around Ku70-Ku80 heterodimers and regulated accessory factors, including long non-coding RNAs, through poorly understood mechanisms. Here, we use magnetic tweezers to investigate contributions core NHEJ proteins APLF lncRNA NIHCOLE synapsis. stabilizes bridging and, together with Ku70-Ku80, establishes minimal that...
The multi-subunit DNA-dependent protein kinase (DNA-PK), a crucial player in DNA repair by non-homologous end-joining higher eukaryotes, consists of catalytic subunit (DNA-PKcs) and the Ku heterodimer. recruits DNA-PKcs to double-strand breaks, where DNA-PK assembles prior repair. interaction with is regulated via autophosphorylation. Recent SAXS data addressed conformational changes occurring purified upon Here, we present first structural analysis effects autophosphorylation on trimeric...
Detection of cytosolic DNA is a central element the innate immunity system against viral infection. The Ku heterodimer, component NHEJ pathway repair in nucleus, functions as sensor that detects dsDNA viruses replicate cytoplasm. Vaccinia virus expresses two proteins, C4 and C16, inactivate sensing enhance virulence. structural basis for this unknown. Here we determine structure C16 - complex using cryoEM. binds by preformed ring but sterically blocks access route, abrogating binding to end...
The phosphatidylinositol 3-kinase-like kinases (PIKKs) are large serine-threonine protein with a catalytic domain homologous to the 3-kinase (PI3K). All PIKK family members share general organization comprising conserved C-terminus that contains PI3K domain, which is preceded by N-terminal region made of helical HEAT repeats. In humans, includes six members, play essential roles in various processes including DNA repair and damage signalling (ATM, ATR, DNA-PKcs), control cell growth (mTOR),...
<div>Abstract<p>Long noncoding RNAs (lncRNA) are emerging as key players in cancer parts of poorly understood molecular mechanisms. Here, we investigated lncRNAs that play a role hepatocellular carcinoma (HCC) and identified NIHCOLE, novel lncRNA induced HCC with oncogenic potential the ligation efficiency DNA double-stranded breaks (DSB). NIHCOLE expression was associated poor prognosis survival patients. Depletion from cells led to impaired proliferation increased apoptosis....
<p>Supplementary Figures S1-S7</p>