A5060642903- Bacteriophages and microbial interactions
- Bacterial Genetics and Biotechnology
- Lysosomal Storage Disorders Research
- DNA Repair Mechanisms
- Escherichia coli research studies
- RNA and protein synthesis mechanisms
- Viral gastroenteritis research and epidemiology
- Trypanosoma species research and implications
- Glycogen Storage Diseases and Myoclonus
- Carbohydrate Chemistry and Synthesis
- Enzyme Structure and Function
- Genomics and Phylogenetic Studies
- Cellular transport and secretion
- Mitochondrial Function and Pathology
- Genetic Neurodegenerative Diseases
- Antibiotic Resistance in Bacteria
- Carcinogens and Genotoxicity Assessment
- Child Nutrition and Feeding Issues
- Microbial infections and disease research
- Advanced biosensing and bioanalysis techniques
- Bacterial biofilms and quorum sensing
- Metabolism and Genetic Disorders
- Vibrio bacteria research studies
- Folate and B Vitamins Research
- Autophagy in Disease and Therapy
University of Gdańsk
2016-2025
MRC Laboratory of Molecular Biology
2001-2025
Loyola University Medical Center
2021
Loyola University Chicago
2021
Institute of Biochemistry and Biophysics, Polish Academy of Sciences
1996-2019
Zero to Three
2016
Gdańsk Medical University
2011
Polish Academy of Sciences
1995-2008
Instytut Oceanologii Polskiej Akademii Nauk
2002-2008
University of Birmingham
2007
Abstract A large scale analysis presented in this article focuses on biological and physiological variety of bacteriophages. collection 83 bacteriophages, isolated from urban sewage able to propagate cells different bacterial hosts, has been obtained (60 infecting Escherichia coli , 10 Pseudomonas aeruginosa 4 Salmonella enterica 3 Staphylococcus sciuri 6 Enterococcus faecalis ). High diversity the is indicated by its characteristics, both morphological (electron microscopic analyses) (host...
Although it is estimated that 20-30% of the general human population are carriers Staphylococcus aureus, this bacterium one most important etiological agents responsible for healthcare-associated infections. The appearance methicillin resistant S. aureus (MRSA) strains has created serious therapeutical problems. Detailed understanding mechanisms infections seems necessary to develop new effective therapies against pathogen. In article, we present an overview biochemical and genetic...
Background Neurodegenerative metabolic disorders such as mucopolysaccharidosis IIIB (MPSIIIB or Sanfilippo disease) accumulate undegraded substrates in the brain and are often unresponsive to enzyme replacement treatments due impermeability of blood barrier enzyme. MPSIIIB is characterised by behavioural difficulties, cognitive later motor decline, with death second decade life. Most these neurodegenerative lysosomal storage diseases lack effective treatments. We recently described...
Abstract The spread of antimicrobial resistant bacterial pathogens combined with the lack new drug classes in antibiotic pipeline causes a resurgence use viruses (phages) to treat infections (phage therapy [PT]). There has been substantial increase patients subjected this experimental and emergence PT centers Europe United States paralleled by one clinical trial completed accord good medical practice (GMP) requirements few others underway. What is more, evidence accumulating suggest that...
Usnic acid, a compound produced by various lichen species, has been demonstrated previously to inhibit growth of different bacteria and fungi; however, mechanism its antimicrobial activity remained unknown. In this report, we demonstrate that usnic acid causes rapid strong inhibition RNA DNA synthesis in Gram-positive bacteria, represented Bacillus subtilis Staphylococcus aureus, while it does not production macromolecules (DNA, RNA, proteins) Escherichia coli, which is resistant even high...
One of the hopes for overcoming antibiotic resistance crisis is use bacteriophages to combat bacterial infections, so-called phage therapy. This therapeutic approach generally believed be safe humans and animals as phages should infect only prokaryotic cells. Nevertheless, recent studies suggested that might recognized by eukaryotic cells, inducing specific cellular responses. Here we show in chickens infected with Salmonella enterica treated a cocktail, are initially animal cells viruses,...
Proteasomes catalyze protein degradation in cells and play an integral role cellular homeostasis. Its activity decreases with age alongside the load of defective proteins, resulting from mutations or oxidative stress-induced damage. Such proteins are prone to aggregation and, if not efficiently degraded, can form toxic oligomers amyloid plaques. Developing effective way activate proteasome could prevent such pathologies. Designing activators is easy because they do bind active site, which...
Sanfilippo disease (mucopolysaccharidosis type III [MPS III]) is a rare neurodegenerative metabolic caused by deficiency of 1 the 4 enzymes involved in degradation heparan sulfate (HS), glycosaminoglycan (GAG). Genistein has been proposed as potential therapy but its efficacy remains uncertain. We aimed to determine genistein MPS III.Thirty patients were enrolled. Effects determined randomized, crossover, placebo-controlled intervention with genistein-rich soy isoflavone extract (10mg/kg/day...
Mucopolysaccharidoses (MPS) are inherited metabolic disorders caused by mutations leading to dysfunction of one enzymes involved in degradation glycosaminoglycans (GAGs). Due their impaired degradation, GAGs accumulate cells patients, which results tissues and organs. Substrate reduction therapy is potential treatment these diseases. It was demonstrated previously that genistein (4', 5, 7-trihydroxyisoflavone) inhibits synthesis reduces levels cultures fibroblasts MPS patients. Recent pilot...