A5113593266- RNA Research and Splicing
- Axon Guidance and Neuronal Signaling
- Cell Adhesion Molecules Research
- Monoclonal and Polyclonal Antibodies Research
- Advanced biosensing and bioanalysis techniques
- Cancer-related gene regulation
- RNA Interference and Gene Delivery
- Viral Infectious Diseases and Gene Expression in Insects
- Hepatitis B Virus Studies
- Advanced Biosensing Techniques and Applications
- Hippo pathway signaling and YAP/TAZ
- Immunotherapy and Immune Responses
- RNA modifications and cancer
- Wnt/β-catenin signaling in development and cancer
- Toxin Mechanisms and Immunotoxins
- Cancer-related molecular mechanisms research
- Research on Leishmaniasis Studies
- Apelin-related biomedical research
- Galectins and Cancer Biology
- Ubiquitin and proteasome pathways
- Immune Response and Inflammation
- Neonatal Respiratory Health Research
- Genomics and Chromatin Dynamics
- Cardiomyopathy and Myosin Studies
- Barrier Structure and Function Studies
Nagoya University
2012-2024
Japan Science and Technology Agency
2003-2006
Aichi Medical University
2004-2006
National Institutes of Health
2002-2005
National Cancer Institute
2001
T/EBP/NKX2.1, a member of the NKX family homeodomain-containing transcription factors, regulates expression number genes in lung and thyroid. Here we describe isolation characterization novel target gene, termed claudin-18, that is down-regulated lungs T/ebp/Nkx2.1-null mouse embryos. The gene product exhibits an amino acid sequence similar to those claudin multigene proteins constitute tight junction strands epithelial cells. was localized by fluorescence situ hybridization chromosome 9 at...
When establishing the most appropriate cells from huge numbers of a cell library for practical use in regenerative medicine and production various biopharmaceuticals, heterogeneity often found an isogenic population limits refinement clonal culture. Here, we demonstrated high-throughput screening suitable by automated undisruptive single-cell analysis isolation system, followed expansion isolated single cells. This system enabled establishment cells, such as embryonic stem with highest...
Activated HSCs (hepatic stellate cells) are the main source of extracellular matrix proteins present in cirrhotic liver on which HCC (hepatocellular carcinoma) commonly develops. cells behave differently according to differences surrounding microenvironment. In study, we have investigated a mechanism whereby modulate migratory activity cells. We used primary cultures human investigate their effect Hep3B, Alexander, HLE and HLF The expression Ln-5 (laminin-5) was documented at transcript...
Laminin-1 is the major component of embryonic basement membrane and consists α1, β1, γ1 chains. The expression laminin-1 induced in mouse F9 embryonal carcinoma cells upon differentiation into parietal endoderm cells. We recently identified a endoderm-specific enhancer laminin α1 (Lama1) gene showed that Sp1/Sp3 YY1 transcription factors were involved activity. Although here we NF-Y binds to sequence between Sp1/Sp3- YY1-binding sites, all these are ubiquitously expressed thus not sufficient...
Accumulating evidence suggests that the delivery of human adipose tissue–derived stromal cells (hASCs) has great potential as regenerative therapy. This was performed to develop a method for expanding hASCs by reducing amount serum required. We demonstrate were able expand efficiently in media containing 2% and fibroblast growth factor-2. These cells, or low cultured (hLASCs), expressed cell surface markers similar those on bone marrow–derived mesenchymal stem could be differentiated into...
A novel gene that is down-regulated in lungs of T/ebp/Nkx2.1-null mouse embryos has been identified using a suppressive-subtractive hybridization method.The product secreted protein, forms homodimer, and exhibits an amino acid sequence similar to seen the uteroglobin/Clara cell secretory protein family proteins.This gene, designated Ugrp1 (uteroglobin-related 1), consists three exons two introns produces transcripts by alternative splicing.The was localized fluorescence situ chromosome 18 at...
Abstract NELL‐1 (Nel‐like molecule‐1) is a secreted osteogenic growth factor first identified in human craniosynostosis (CS) patients. protein has been observed to promote bone and cartilage differentiation suppress adipogenesis both vitro vivo models. Despite these findings, the cell surface receptors of have remained unknown. In this study, we for time that promotes adherence multiple lines, including ST2, C3H10T1/2, M2‐10B4, ATDC5, MC3T3 cells. Additionally, found binds extracellular...
Oriented immobilization of sensing molecules on solid phases is an important issue in biosensing. In case immunosensors, it essential to scrutinize not only the direction and shape immunoglobulin G (IgG) solution but also real-time movement IgGs, which cannot be achieved by conventional techniques. Recently, we developed bio-nanocapsules (BNCs) displaying a tandem form IgG Fc-binding Z domain derived from Staphylococcus aureus protein A (ZZ-BNC) enhance sensitivity antigen-binding capacity...
Claudin-18 ( CLDN18), a member of the claudin family proteins that are structural components tight junctions, has two alternatively spliced variants, claudin-18a1 and claudin-18a2, which highly expressed in lung stomach, respectively. Downregulation claudin-18a2 is associated with gastric cancers an intestinal phenotype; however, mechanisms regulating its expression have not been defined. Here, we found phorbol 12-myristate 13-acetate (PMA) treatment MKN45 human cancer cell line increased...
For establishing cells that secrete antibodies most efficiently (e.g., hybridomas, CHO (Chinese hamster ovary) cells), the screening and subsequent breeding of promising have been performed at single-colony level, which requires several weeks to propagate a substantial number by forming colonies from single for evaluation conventional assays. However, this process lacks high-throughput performance in time colony numbers. Therefore, development novel methods is expected identify secreting...
Molybdenum (Mo) is an essential trace element for almost all living organisms including animals. Mo used as a catalytic center of molybdo-enzymes oxidation/reduction reactions carbon, nitrogen, and sulfur metabolism. Whilst cells are known to import inorganic molybdate oxyanion from the surrounding environment, in vivo dynamics cytosolic remain poorly understood no appropriate indicator available this anion. We here describe genetically encoded Förester-resonance-energy-transfer (FRET)-based...
NELL1 is a secretory osteogenic protein containing several structural motifs that suggest it functions as an extracellular matrix component. To determine the mechanisms underlying NELL1‐induced osteoblast differentiation, we examined cell‐adhesive activity of using series recombinant proteins. We demonstrated promoted osteoblastic cell adhesion through at least three cell‐binding domains located in C‐terminal region NELL1. Adhesion cells to was strongly inhibited by function‐blocking...
Abstract: Bionanocapsules (BNCs) are hollow nanoparticles consisting of hepatitis B virus (HBV) envelope L proteins and have been shown to deliver drugs genes specifically human hepatic tissues by utilizing HBV-derived infection machinery. The complex BNCs with liposomes (LPs), the BNC–LP complexes (a LP surrounded in a rugged spherical form), could also become active targeting nanocarriers BNC function. In this study, under acidic conditions high temperature, were found fully fuse LPs...