A5068986976- Microbial Community Ecology and Physiology
- Environmental DNA in Biodiversity Studies
- Marine and coastal ecosystems
- Methane Hydrates and Related Phenomena
- Marine and coastal plant biology
- Protist diversity and phylogeny
- Marine Bivalve and Aquaculture Studies
- Identification and Quantification in Food
- Arctic and Antarctic ice dynamics
- Genomics and Phylogenetic Studies
- Bacteriophages and microbial interactions
- Isotope Analysis in Ecology
- Ocean Acidification Effects and Responses
- Gut microbiota and health
- Marine Biology and Ecology Research
- Climate change and permafrost
- Coastal and Marine Management
- Molecular Biology Techniques and Applications
- RNA modifications and cancer
- Aquaculture disease management and microbiota
- Soil and Water Nutrient Dynamics
- Geology and Paleoclimatology Research
- Parasite Biology and Host Interactions
- Hydrology and Watershed Management Studies
- RNA and protein synthesis mechanisms
Tula Foundation
2018-2025
Hakai Institute
2018-2025
University of British Columbia
2017-2024
Genomics (United Kingdom)
2024
Victoria Heart Institute Foundation
2023
Oregon State University
2014-2015
University of Washington
2009-2014
AME Aquatic Microbial Ecology Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout JournalEditorsSpecials 57:1-18 (2009) - DOI: https://doi.org/10.3354/ame01317 Comparison of free-living, suspended particle, and aggregate-associated bacterial archaeal communities in Laptev Sea Colleen T. E. Kellogg*, Jody W. Deming School Oceanography, University Washington, Box 357940, Seattle, Washington 98195, USA *Email:...
Warming at nearly twice the global rate, higher than average air temperatures are new 'normal' for Arctic ecosystems. This rise in temperature has triggered hydrological and geochemical changes that increasingly release carbon-rich water into coastal ocean via increased riverine discharge, erosion, thawing of semi-permanent permafrost ubiquitous region. To determine biogeochemical impacts terrestrially derived dissolved organic matter (tDOM) on marine ecosystems we compared nutrient stocks...
Snow overlays the majority of Greenland Ice Sheet (GrIS). However, there is very little information available on microbiological assemblages that are associated with this vast and climate-sensitive landscape. In study, structure diversity snow microbial from two regions western GrIS ice margin were investigated through sequencing small subunit ribosomal RNA genes. The origins microbiota by examining correlations to molecular data obtained marine, soil, freshwater atmospheric environments...
The Pacific coastal temperate rainforest (PCTR) is a global hot-spot for carbon cycling and export. Yet the influence of microorganisms on processes in PCTR soil poorly characterized. We developed tested conceptual model seasonal microbial through integration geochemistry, micro-meteorology, eukaryotic prokaryotic ribosomal amplicon (rRNA) sequencing from 216 DNA RNA libraries. Soil moisture pH increased during wet season, with significant correlation to net CO2 flux peat bog CH4 forest...
Abstract During the outmigration of Pacific Salmon, early marine phase is a critical period when high mortality can occur. Traditional sampling and monitoring juvenile salmon migration be limited by logistically intensive gear requirements, accessibility, cost. Improved understanding phase, for example, duration habitat use, requires innovative techniques that improve spatial temporal coverage monitoring. Environmental DNA (eDNA) genetic fragments present in environment used as proxy...
Abstract Marine biodiversity is a key indicator of ecosystem health and can be assessed using variety methods, including environmental DNA (eDNA) sampling. However, the ecology eDNA in physically dynamic nearshore environments remains uncertain, particularly with regards to how stratifies depth. Here, we paired sampling dive surveys at six sites Knight Inlet, British Columbia, Canada. samples were collected from surface, midwater column bottom (8–25 m depth) each site, while focused on...
Microbial enzymatic hydrolysis of marine-derived particulate organic carbon (POC) can be a dominant mechanism for attenuating flux in cold Arctic waters during spring and summer. Whether this depends on composition associated microbial communities extends into other seasons is not known. Bacterial community (BCC) extracellular enzyme activity (EEA, leucine aminopeptidases, glucosidases chitobiases) were measured small suspended particles potentially sinking aggregates collected fall from the...
Although previous studies, mostly based on microscopy analyses of a few groups protists, have suggested that protists are abundant and diverse in litter moss habitats, the overall diversity associated remains elusive. Here, high-throughput environmental sequencing was used to characterize community structure litter- moss-associated along gradient soil drainage forest primary productivity temperate rainforest British Columbia. We identified 3262 distinct protist OTUs from 36 sites. Protists...
Large changes in marine CO2 chemistry manifest areas with weakly-buffered seawater where ocean acidification (OA) acts concert natural additions. These settings can exhibit periods of extreme OA the form multiple co-occurring stressors, including calcite undersaturation and low pH. Such conditions were observed northern Strait Georgia, on northeast Pacific coast, spanned a 3-year period. Here, we utilized an 8-year, highly-resolved record partial pressure total dissolved inorganic carbon to...
Abstract In this study, we evaluated how well DNA metabarcoding of environmental samples captures changes in marine mesozooplankton community composition to optimize the use sequencing data for studying seasonal dynamics. Although is increasingly used monitor distribution communities, there a lack standardized methods, and it remains uncertain what extent reflects patterns dynamics observed by other methods. Zooplankton net were collected every second week throughout 2017 northern Salish...
Abstract Watersheds of the coastal temperate rainforests Pacific North America export large amounts organic carbon (OC) to ocean. While it has been suggested that terrestrially derived matter could subsidize marine food webs and affect ocean biogeochemistry along margin, little work done quantify characterize OC across freshwater continuum. We conducted monthly targeted rainfall event surveys dissolved particulate (DOC POC) quantity quality (δ 13 C, characterization) a salinity gradient...
The impact of marine heatwaves (MHWs) on ecosystem functions and services remains poorly constrained due in part to the limited availability time-resolved datasets integrating physical, chemical biological parameters at relevant operating scales. Here we paired over a decade autonomous Biogeochemical (BGC)-Argo profiling float data with water column plankton community composition profiles investigate impacts MHWs carbon cycling dynamics Northeastern subarctic Pacific Ocean (NESAP), resolving...
Abstract Recent studies on marine heat waves describe water temperature anomalies causing changes in food web structure, bloom dynamics, biodiversity loss, and increased plant animal mortality. However, little information is available how impact prokaryotes (bacteria archaea) inhabiting ocean waters. This a nontrivial omission given their integral roles driving major biogeochemical fluxes that influence productivity the climate system. Here we present time-resolved study of large-scale warm...
MEPS Marine Ecology Progress Series Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout JournalEditorsTheme Sections 527:31-45 (2015) - DOI: https://doi.org/10.3354/meps11207 Seasonal changes in quantity and composition of suspended particulate organic matter lagoons Alaskan Beaufort Sea Tara L. Connelly1,*, James W. McClelland1, Byron C. Crump2, Colleen T. E. Kellogg2,3, Kenneth H. Dunton1 1Marine Science Institute,...
Abstract Protists (eukaryotic microorganisms) commonly form symbiotic associations with crustacean zooplankton, but their diversity, prevalence and ecological roles are underestimated due to the limited scope of previous investigations. Using metabarcoding V4 region 18S rRNA gene, we characterized eukaryotic microbiomes dominant specifically copepods, euphausiids, amphipods ostracods, from Strait Georgia, Canada. Sequence reads alveolates dominated all zooplankton examined, which mostly...
Abstract Land and ocean ecosystems are strongly connected mutually interactive. As climate changes other anthropogenic stressors intensify, the complex pathways that link these systems will strengthen or weaken in ways currently beyond reliable prediction. In this review we offer a framework of land–ocean couplings their role shaping marine coastal temperate rainforest (CTR) ecoregions, where high freshwater materials flux result particularly strong connections. Using largest contiguous...
Abstract The Kwakshua Watersheds Observatory (KWO) is an integrative watersheds observatory on the coastal margin of a rain‐dominated bog‐forest landscape in British Columbia (BC), Canada. Established 2013, goal KWO to understand and model flux terrestrial materials from land sea – origins, pathways, processes ecosystem consequences context long‐term environmental change. consists seven gauged network observation sites spanning along drainage gradients within catchments. Time‐series datasets...
Shellfish industries are threatened worldwide by recurrent summer mortality events. Such incidences often associated with Vibrio disease outbreaks, and thus, it is critical that animals able to mount sufficient immune responses.
This protocol describes water filtrations onto 0.22μl sterivex filters using a peristaltic pump. As part of the Hakai Institute Ocean Observing Program, biomolecular samples have been collected weekly, from 0 m to near bottom (260 m), genetically characterize plankton communities in Northern Salish Sea since 2015. is developed work across all domains life, viruses prokaryotes eukaryotes, allowing for both amplicon sequencing and shotgun sequencing.
This protocol is used for eDNA metabarcoding of the 18S SSU rRNA Gene (Balzano et al 2015) using Pair-End Illumina Miseq. Sequencing. As part Hakai Institute Ocean Observing Program, biomolecular samples have been collected weekly, from 0 m to near bottom (260 m), genetically characterize plankton communities in Northern Salish Sea since 2015. developed provide taxonomic annotations Eukaryote Nuclear DNA.
This protocol is used for eDNA metabarcoding of the Cytochrome Oxidase I (COI) "Leray fragment", using Pair-End Illumina MiseqSequencing. As part Hakai Institute Ocean Observing Program, biomolecular samples have been collected weekly, from 0 m to near bottom (260 m), genetically characterize plankton communities in Northern Salish Sea since 2015. developed give a species-level resolution marine invertebrates.
This protocol is used to prepare low-cost SPRI beads for Illumina Library preparations. As part of the Hakai Institute Ocean Observing Program, from 0 m near bottom (260 m), biomolecular samples have been collected weekly genetically characterize plankton communities in Northern Salish Sea since 2015. These clean up PCR products 16S, 18S, COI, and 12S amplicons, implemented as a standard procedure eDNA analysis. modification following protocol: B. Faircloth T. Glenn. Serapure v2.2. Ecol....