A5055793389- Epigenetics and DNA Methylation
- Renal and related cancers
- Sperm and Testicular Function
- Reproductive Biology and Fertility
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Genetic Syndromes and Imprinting
- Pluripotent Stem Cells Research
- Sports injuries and prevention
- Retinopathy of Prematurity Studies
- Immunotherapy and Immune Responses
- Tissue Engineering and Regenerative Medicine
- Genomics and Chromatin Dynamics
- Shoulder Injury and Treatment
- Tendon Structure and Treatment
- Cancer Genomics and Diagnostics
- Chromatin Remodeling and Cancer
- CRISPR and Genetic Engineering
- Cancer Cells and Metastasis
- Corneal Surgery and Treatments
- Retinal Development and Disorders
Chinese University of Hong Kong
2018-2024
Shandong University
2019
Neonatal germ cell development provides the foundation of spermatogenesis. However, a systematic understanding this process is still limited. To resolve cellular and molecular heterogeneity in process, we profiled single transcriptomes undifferentiated cells from neonatal mouse testes employed unbiased clustering pseudotime ordering analysis to assign distinct states developmental continuum. We defined unique transcriptional programs underlying migratory capacity, resting apoptosis...
Glaucoma is characterized by retinal ganglion cell (RGC) degeneration and the second leading cause of blindness worldwide. However, current treatments such as eye drop or surgery have limitations do not target loss RGC. Regenerative therapy using embryonic stem cells (ESCs) holds a promising option, but ethical concern hinders clinical applications on human subjects. In this study, we employed spermatogonial (SSCs) an alternative source ESCs for cell-based regenerative in mouse glaucoma...
Spermatogonial stem cells (SSC), the foundation of spermatogenesis and male fertility, possess lifelong self-renewal activity. Aging leads to decline in cell function increased risk paternal age-related genetic diseases. In present study, we performed a comparative genomic analysis mouse SSC-enriched undifferentiated spermatogonia (Oct4-GFP+/KIT-) differentiating progenitors (Oct4-GFP+/KIT+) isolated from young aged testes. Our transcriptome data revealed enormous complexity expressed coding...
Abstract Background Tendon healing is clinically challenging largely due to its inferior regenerative capacity. We have previously prepared a soluble, DNA-free, urea-extracted bovine tendon-derived extracellular matrix (tECM) that exhibits strong pro-tenogenic bioactivity on human adipose-derived stem cells (hASCs). In this study, we aimed elucidate the mechanism of tECM via characterization protein composition and comparison transcriptomic profiles hASC cultures treated with versus collagen...
Spermatogenesis depends on an orchestrated series of developing events in germ cells and full maturation the somatic microenvironment. To date, majority efforts to study cellular heterogeneity testis has been focused single-cell gene expression rather than chromatin landscape shaping expression. advance our understanding regulatory programs underlying testicular cell types, we analyzed accessibility profiles more 25,000 from mouse testis. We showed that sequencing assay for...
Abstract Background Epithelial-mesenchymal transition (EMT) is a phenomenon in which epithelial cells acquire mesenchymal traits. It contributes to organogenesis and tissue homeostasis, as well stem cell differentiation. Emerging evidence indicates that heterogeneous expression of EMT gene markers presents sub-populations germline (GSCs). However, the functional implications such heterogeneity are largely elusive. Results We unravelled an EMT-like process GSCs by vitro extracellular matrix...
Abstract Gonad development includes sex determination and divergent maturation of the testes ovaries. Recent advances in measuring gene expression single cells are providing new insights into this complex process. However, underlying epigenetic regulatory mechanisms remain unclear. Here, we profiled chromatin accessibility mouse gonadal both sexes from embryonic day 11.5 to 14.5 using single-cell assay for transposase accessible by sequencing (scATAC-seq). Our results showed that individual...
ABSTRACT Spermatogonial stem cells (SSC), the foundation of spermatogenesis and male fertility, possess lifelong self-renewal activity. Aging leads to decline in cell function increased risk paternal age-related genetic diseases. In present study, we performed a comparative genomic analysis mouse SSC (Oct4-GFP+/KIT-) differentiating progenitors (Oct4-GFP+/KIT+) isolated from young aged testes. Our transcriptome data revealed enormous complexity expressed coding non-coding RNAs alternative...
Embryonic stem cells (ESCs) have a wide range of clinical applications. However, its ethical concerns cannot be neglected. In this study, we collected spermatogonial (SSCs) from mouse testes and they were subsequently transdifferentiated to ESC‐like through non‐viral non‐invasive approach. These SSC‐derived exhibited cell properties similar ESCs in terms morphology pluripotency gene expression profile. Next, aimed generate retinal ganglion using cell‐derived embryonic (SSC‐ES) cells....
Evaluating oocyte quality in assisted reproductive technology (ART) is essential to ensure a healthy pregnancy. Currently, morphological assessment on applied predict and embryo development potential. The method limited may not provide an accurate of quality. Human polar body (PB) the byproduct maturation. advent PB karyotyping has served as indirect genetic analysis oocytes provided metrics embryonic This prompts idea genome interrogation by studying genome, recent study shown transcriptome...
Spermatogonial stem cells are the most primitive spermatogonia in testis, which can self-renew to maintain cell pool or differentiate give rise germ including haploid spermatids. All-trans-retinoic acid (RA), a bioactive metabolite of vitamin A, plays fundamental role initiating spermatogonial differentiation. In this study, single-cell ATAC-seq (scATAC-seq) was used obtain genome-wide chromatin maps cultured germline (GSCs) that were control and RA-induced differentiation states. We showed...
Abstract Spermatogenesis depends on an orchestrated series of developing events in germ cells and full maturation the somatic microenvironment. To date, majority efforts to study cellular heterogeneity testis has been focused single-cell gene expression rather than chromatin landscape shaping expression. advance our understanding regulatory programs underlying testicular cell types, we analyzed accessibility profiles more 25,000 from mouse testis. We showed that scATAC-Seq allowed us...
We hypothesized that polar body can reflect transcriptomic and epigenetic landscape of sibling oocyte. Single-cell parallel methylome transcriptome sequencing (scM&T-seq) is a powerful method simultaneously reveals the at single-cell single-base resolution. Here we applied to reveal oocyte its body. conducted RNA (scRNA) for human oocytes bodies. However, in cannot oocytes. Therefore, investigated Whole Genome Bisulfite Sequencing (scWGBS) demonstrated good genome coverage both found has...
Abstract Study question What is the transcriptome signature associated with rescuein vitro matured (rIVM) oocytes? Summary answer GATA–1/CREB1/WNT signaling axis was repressed in rIVM oocytes of poor quality. known already aims to produce mature (MII) for fertilization (IVF) through IVM immature collected from stimulated ovaries. It less popular due limited success rate infertility treatment. Genetic aberrations, cellular stress, and absence cumulus cell support could account failure rIVM....