A5091793545- Reproductive Biology and Fertility
- Epigenetics and DNA Methylation
- Sperm and Testicular Function
- Tuberculosis Research and Epidemiology
- Renal and related cancers
- Bacterial Identification and Susceptibility Testing
- Genomics and Phylogenetic Studies
- CO2 Reduction Techniques and Catalysts
- Social Media and Politics
- Advanced Data Compression Techniques
- Age of Information Optimization
- Reproductive System and Pregnancy
- Cancer therapeutics and mechanisms
- Burkholderia infections and melioidosis
- Gut microbiota and health
- Pluripotent Stem Cells Research
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Advanced Image Processing Techniques
- Chemical Looping and Thermochemical Processes
- Corneal Surgery and Treatments
- Retinal Development and Disorders
- Click Chemistry and Applications
- biodegradable polymer synthesis and properties
- Video Coding and Compression Technologies
- Microplastics and Plastic Pollution
Hong Kong Polytechnic University
2018-2024
University of Hong Kong
2024
Pamela Youde Nethersole Eastern Hospital
2024
Chinese University of Hong Kong
2018-2021
Shandong University
2019
City University of Hong Kong
1998-2016
Neonatal germ cell development provides the foundation of spermatogenesis. However, a systematic understanding this process is still limited. To resolve cellular and molecular heterogeneity in process, we profiled single transcriptomes undifferentiated cells from neonatal mouse testes employed unbiased clustering pseudotime ordering analysis to assign distinct states developmental continuum. We defined unique transcriptional programs underlying migratory capacity, resting apoptosis...
Glaucoma is characterized by retinal ganglion cell (RGC) degeneration and the second leading cause of blindness worldwide. However, current treatments such as eye drop or surgery have limitations do not target loss RGC. Regenerative therapy using embryonic stem cells (ESCs) holds a promising option, but ethical concern hinders clinical applications on human subjects. In this study, we employed spermatogonial (SSCs) an alternative source ESCs for cell-based regenerative in mouse glaucoma...
Spermatogonial stem cells (SSC), the foundation of spermatogenesis and male fertility, possess lifelong self-renewal activity. Aging leads to decline in cell function increased risk paternal age-related genetic diseases. In present study, we performed a comparative genomic analysis mouse SSC-enriched undifferentiated spermatogonia (Oct4-GFP+/KIT-) differentiating progenitors (Oct4-GFP+/KIT+) isolated from young aged testes. Our transcriptome data revealed enormous complexity expressed coding...
Microbes in the built environment have been implicated as a source of infectious diseases. Bacterial culture is standard method for assessing risk exposure to pathogens urban environments, but this only accounts <1% diversity bacteria. Recently, full-length 16S rRNA gene analysis using nanopore sequencing has applied microbial evaluations, resulting rise development long-read taxonomic tools species-level classification. Regarding their comparative performance, there is, however, lack...
What is the transcriptome signature associated with poor performance of rescue IVM (rIVM) oocytes and how can we rejuvenate them?The GATA-1/CREB1/WNT signalling axis was repressed in rIVM oocytes, particularly those quality; restoration this may produce more usable oocytes.rIVM aims to mature (MII) for IVF through immature collected from stimulated ovaries. It not popular due limited success rate infertility treatment. Genetic aberrations, cellular stress absence cumulus cell support could...
Objective: This study aimed to characterize the changing landscape of circulating SARS-CoV-2 lineages in local community Hong Kong throughout 2022. We examined how adjustments quarantine arrangements influenced transmission pattern Omicron variants a city with relatively rigorous social distancing measures at that time. Methods: In 2022, total 4684 genomes were sequenced using Oxford Nanopore GridION sequencer. consensus generated by MAFFT, and maximum likelihood phylogeny these was...
Between January 2015 and October 2022, 38 patients with culture-confirmed melioidosis were identified in the Kowloon West (KW) Region, Hong Kong. Notably, 30 of them clustered Sham Shui Po (SSP) district, which covers an estimated area 2.5 km2. August 18 this district after heavy rainfall typhoons. The sudden upsurge cases prompted environmental investigation, involved collecting 20 air samples 72 soil from residential areas near patients. A viable isolate Burkholderia pseudomallei was...
Objective: This study evaluated the capability of a MALDI Biotyper system equipped with newly introduced MBT STAR-BL module to simultaneously perform species identification and β-lactamase-mediated resistance detection in bacteremia -causing bacteria isolated from cultured isolates patient-derived blood cultures (BCs). Methods: Two hundred retrospective 153 prospective BCs containing Gram-negative rods (GNR) were collected subjected direct bacterial identification, followed by measurement...
The emergence of multidrug-resistant strains and hyper-virulent Mycobacterium tuberculosis are big therapeutic challenges for (TB) control. Repurposing bioactive small-molecule compounds has recently become a new approach against TB. This study aimed to identify novel anti-TB agents from library via rapid screening system. A total 320 were used screen their ability suppress the expression key virulence gene, phop, M. complex using luminescence (lux)-based promoter-reporter platforms. minimum...
Clinical manifestations of tuberculosis range from asymptomatic infection to a life-threatening disease such as tuberculous meningitis (TBM). Recent studies showed that the spectrum severity could be related genetic diversity among clinical strains Mycobacterium (Mtb). Certain are reported preferentially invade central nervous system, thus earning label “hypervirulent strains”.However, specific mutations accounted for enhanced mycobacterial virulence still unknown. We previously identified...
Abstract Background Epithelial-mesenchymal transition (EMT) is a phenomenon in which epithelial cells acquire mesenchymal traits. It contributes to organogenesis and tissue homeostasis, as well stem cell differentiation. Emerging evidence indicates that heterogeneous expression of EMT gene markers presents sub-populations germline (GSCs). However, the functional implications such heterogeneity are largely elusive. Results We unravelled an EMT-like process GSCs by vitro extracellular matrix...
Objective: The study aimed to characterize the changing landscape of circulating SARS-CoV-2 lineages in local community Hong Kong throughout 2022. We examined how adjustments quarantine arrangements influenced transmission pattern Omicron variants a city with relatively rigorous social distancing measures at that time. Methods: In 2022, total 4,684 genomes were sequenced using Oxford Nanopore GridION sequencer. consensus generated by MAFFT, and maximum likelihood phylogeny these determined...
Abstract Foodborne pathogens, particularly antimicrobial-resistant (AMR) bacteria, remain a significant threat to global health. Conventional culture-based approaches for detecting infectious agents are limited in scope and time-consuming. Metagenomic sequencing of food products offers rapid comprehensive approach detect pathogenic microbes, including AMR bacteria. In this study, we used nanopore-based metagenomic microbes antimicrobial resistance genes (ARGs) 260 products, raw meat,...
Female fertility declines dramatically with age, and childbearing at advanced maternal age is a big health concern due to greater risk of early pregnancy‐related complications such as miscarriage, genetic disorders, ectopic pregnancy. The negative impact on caused by aging also implicated in assisted reproduction technologies. A major factor that accounts for deterioration older women poor oocyte quality. By applying RNA‐seq, we compared transcriptome profiles GV MII oocytes from the young...
Abstract Background Advanced maternal aging has become a worldwide public health issue that contributes to female fertility decline and significant risk embryo development. Despite transcriptional epigenetic alterations reported in oocyte maturation development, the dynamics of gene expression DNA associated with remain elusive. Here we generated simultaneous transcriptome methylome profiles mouse oocytes during at single-cell single-base resolution examine key biological processes identify...
Still picture coding techniques such as adaptive interpolative are always applied to intraframe image compression in video algorithms. Coding fidelity is utilized measuring the performance of still methods. In this paper, a popular motion estimation method known block matching algorithm (BMA) adopted reconstructing interframe images based on contents encoded with different techniques. The compatibility can be reflected from fidelities images. Experimental results show that edge prediction...
Abstract Spermatogenesis depends on an orchestrated series of developing events in germ cells and full maturation the somatic microenvironment. To date, majority efforts to study cellular heterogeneity testis has been focused single-cell gene expression rather than chromatin landscape shaping expression. advance our understanding regulatory programs underlying testicular cell types, we analyzed accessibility profiles more 25,000 from mouse testis. We showed that scATAC-Seq allowed us...
Abstract The emergence of multidrug-resistant strains and hyper-virulent Mycobacterium tuberculosis are big therapeutic challenges for (TB) control. Repurposing bioactive small-molecule compounds has recently become a new approach against TB. This study aimed to construct rapid screening system identify novel anti-TB agents from library compounds. In this study, total 320 were used screen their ability suppress the expression key virulence gene, phoP , M. complex using luminescence ( lux...