A5087630586- SARS-CoV-2 detection and testing
- Biosensors and Analytical Detection
- Bacterial biofilms and quorum sensing
- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Advanced biosensing and bioanalysis techniques
- Microfluidic and Bio-sensing Technologies
- Probiotics and Fermented Foods
- COVID-19 diagnosis using AI
- Gut microbiota and health
- Protein Hydrolysis and Bioactive Peptides
- Advanced Chemical Sensor Technologies
- Antimicrobial Peptides and Activities
- Microbial Community Ecology and Physiology
- Graphene research and applications
- Genomics, phytochemicals, and oxidative stress
- Polymer Surface Interaction Studies
- Gas Sensing Nanomaterials and Sensors
- Phytochemicals and Antioxidant Activities
- Bacterial Genetics and Biotechnology
- Advanced Sensor and Energy Harvesting Materials
- Piperaceae Chemical and Biological Studies
- Lipid Membrane Structure and Behavior
- Bacteriophages and microbial interactions
- Essential Oils and Antimicrobial Activity
Ben-Gurion University of the Negev
2016-2023
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an RNA virus, a member of the family viruses that includes severe 1 (SARS-CoV-1) and Middle East (MERS). It has had dramatic impact on health care systems, economies, societies affected countries during past 8 months. Widespread testing tracing efforts are being employed in many attempts to contain mitigate this pandemic. Recent data indicated fecal shedding SARS-CoV-2 common virus can be detected wastewater. This indicates...
Abstract SARS-CoV-2 is an RNA virus, a member of the coronavirus family respiratory viruses that includes SARS-CoV-1 and MERS. COVID-19, clinical syndrome caused by SARSCoV-2, has evolved into global pandemic with more than 2,900,000 people infected. It had acute dramatic impact on health care systems, economies, societies affected countries within these few months. Widespread testing tracing efforts are employed in many order to contain mitigate this pandemic. Recent data indicated fecal...
Municipal sewage carries degraded and intact viral particles RNA (ribonucleic acid) of SARS-CoV-2 (severe acute respiratory coronavirus 2), shed by COVID-19 (coronavirus disease 2019) patients, to eventually wastewater treatment plants. Proper can prevent uncontrolled discharges the virus into environment. However, role different stages in reducing concentrations is, thus far, unknown. Here, we quantified raw during main activated sludge process from two plants Israel, on three days 2020...
P. aeruginosa flagella are recorded across the entire biofilm life cycle as likely structural scaffolds.
Novel artificial nose based upon electrode-deposited carbon dots (C-dots). Significant selectivity and sensitivity determined by "polarity matching" between the C-dots gas molecules. The C-dot facilitates, for first time, real-time, continuous monitoring of bacterial proliferation discrimination among species, both Gram-positive Gram-negative bacteria specific strains. Machine learning algorithm furnishes excellent predictability in case individual gases complex mixtures. Continuous,...
Quorum sensing (QS), a cell-to-cell communication process, entails the production of signaling molecules that enable synchronized gene expression in microbial communities to regulate myriad functions, including biofilm formation. QS disruption may constitute an innovative approach design novel antifouling and anti-biofilm agents. To identify quorum inhibitors (QSI), 2,500 environmental bacterial artificial chromosomes (BAC) from uncultured marine planktonic bacteria were screened for QSI...
Abstract Background Probiotic milk-fermented microorganism mixtures (e.g., yogurt, kefir) are perceived as contributing to human health, and possibly capable of protecting against bacterial infections. Co-existence probiotic microorganisms likely maintained via complex biomolecular mechanisms, secreted metabolites mediating cell-cell communication, other yet-unknown biochemical pathways. In particular, deciphering molecular mechanisms by which inhibit proliferation pathogenic bacteria would...
Antimicrobial resistance is among the top global health problems with antibacterial currently representing major threat both in terms of occurrence and complexity. One reason current treatments bacterial diseases are ineffective protective resistant biofilm structures. Phytochemicals being reviewed for newer anti-virulence agents. In present study, we aimed to investigate activity 3,3′-diindolylmethane (DIM), a bioactive cruciferous phytochemical. Using series vitro assays on Gram-negative...
Abstract SARS-CoV-2 variants of concern present a worldwide threat. Demonstrating higher infection rate and durability to antibodies when compared the original virus, are responsible for continuing global outbreaks. Prompt identification infecting variant is essential pandemic assessment containment. However, mainly being performed using expensive, time-consuming next generation sequencing. Rapid methodology great need various variant-specific assays developed. Amongst that have recently...
ABSTRACT The municipal sewage carries the new coronavirus (SARS-CoV-2), shed by COVID-19 patients, to wastewater treatment plants. Proper can provide an important barrier for preventing uncontrolled discharged of virus into environment. However, role different stages in reducing concentrations was, thus far, unknown. In this work, we quantified SARS-CoV-RNA raw and along main process from two plants Israel during outbreak. We found that ca. 2 Log removal could be attained after primary...
Abstract Less than a year following the SARS-CoV-2 outbreak, variants of concern have emerged in form British variant B.1.1.7 and South Africa B.1.351. Due to their high infectivity morbidity, it is crucial quickly effectively detect them. Current methods detection are either time-consuming, expensive or indirect. Here, we report development rapid, cost-effective direct RT-qPCR method for two concern. We developed validate system another single set B.1.351 variant. The approach was...
Background Wastewater based epidemiology (WBE) has become an important tool in SARS-CoV-2 surveillance and epidemiology. While WBE measurements generally correlate with observed case numbers large municipal areas on sewer grids, there are few studies its utility communities that off-grid (non-sewered). Methods materials To explore the applicability of wastewater our region, five Bedouin along Hebron Stream Southern Israel (Negev desert) were sampled. One point (El-Sayed) represents a...
Bacterial communication, termed Quorum Sensing (QS), is a promising target for virulence attenuation and the treatment of bacterial infections. Infections cause inflammation, process regulated by number cellular factors, including transcription Nuclear Factor kappa B (NF-κB); this factor found to be upregulated in many inflammatory diseases, those induced infection. In study, we tested 32 synthetic derivatives coumaperine (CP), known natural compound pepper (Piper nigrum), Inhibition (QSI)...
Abstract The breath figure (BF) method is a simple technique for fabricating micropatterned surfaces but has been rarely studied using graphene oxide (GO) or reduced GO (rGO). Additionally, fabrication of rGO (MPGO MPrGO) by the BF focuses on smooth and dense inorganic substrates, investigation adjusting MPGO morphology limited. This research systematically studies self‐assembly MPrGO surface two model porous polymers explores its potential applications modification. It found that size range...
Abstract The COVID-19 pandemic created a global crisis impacting not only healthcare systems, but also world economies and society. Recent data have indicated that fecal shedding of SARS-CoV-2 is common, viral RNA can be detected in wastewater. This suggests wastewater monitoring potentially efficient tool for both epidemiological surveillance, early warning circulation at the population level. In this study we sampled an urban infrastructure city Ashkelon, Israel, during end first wave May...
Abstract The opportunistic pathogen, Pseudomonas aeruginosa , a flagellated bacterium, is one of the top model organisms for studying biofilm formation. In order to elucidate role bacteria flagella in formation, we developed new tool bio-tracking. We have site-specifically labeled bacterial by incorporating an unnatural amino acid into monomer via genetic code expansion. This enabled us label and track during maturation. Direct, live imaging revealed first-time presence synthesis throughout...
Unwelcomed biofilms are problematic in food industries, surgical devices, marine applications, and wastewater treatment plants; essentially everywhere where there is moisture. Very recently, label-free advanced sensors such as localised extended surface plasmon resonance (SPR) have been explored tools for monitoring biofilm formation. However, conventional noble metal SPR substrates suffer from low penetration depth (100 - 300 nm), preventing the reliable detection of large entities single...