A5032519520- Plant Molecular Biology Research
- Plant Reproductive Biology
- Plant Taxonomy and Phylogenetics
- Plant nutrient uptake and metabolism
- Genomics and Phylogenetic Studies
- Photosynthetic Processes and Mechanisms
- Chromosomal and Genetic Variations
- Plant Parasitism and Resistance
- Tree Root and Stability Studies
- Polysaccharides and Plant Cell Walls
- Crop Yield and Soil Fertility
- Plant Gene Expression Analysis
- Wheat and Barley Genetics and Pathology
- Plant tissue culture and regeneration
- ATP Synthase and ATPases Research
- Fern and Epiphyte Biology
- Insect and Arachnid Ecology and Behavior
- Legume Nitrogen Fixing Symbiosis
- Turfgrass Adaptation and Management
- Plant Physiology and Cultivation Studies
- Congenital heart defects research
- Genetic diversity and population structure
Colorado State University
2024
Sainsbury Laboratory
2015-2024
University of Cambridge
2014-2024
Plant Gene Expression Center
2009-2014
United States Department of Agriculture
2014
Agricultural Research Service
2014
University of California, Berkeley
2009-2014
Ollscoil na Gaillimhe – University of Galway
2001
KNOTTED1 (KN1)-like homeobox (KNOX) transcription factors function in plant meristems, self-renewing structures consisting of stem cells and their immediate daughters. We defined the KN1 cistrome maize inflorescences found that binds to several thousand loci, including 643 genes are modulated one or multiple tissues. These direct targets strongly enriched for (including other genes) participating hormonal pathways, most significantly auxin, demonstrating plays a key role orchestrating upper...
BackgroundPlant body plans arise by the activity of meristematic growing tips during development and radiated independently in gametophyte (n) sporophyte (2n) stages life cycle evolution. Although auxin its intercellular transport PIN family efflux carriers are primary regulators sporophytic shoot flowering plants, extent conservation function within land plants mechanisms regulating bryophyte gametophytic largely unknown.ResultsWe have found that treating shoots moss Physcomitrella patens...
The hormone auxin plays a crucial role in plant morphogenesis. In the shoot apical meristem, PIN-FORMED1 (PIN1) efflux carrier concentrates into local maxima epidermis, which position incipient leaf or floral primordia. From these maxima, PIN1 transports internal tissues along emergent paths that pattern and stem vasculature. Arabidopsis thaliana, functions are attributed to single protein. Using phylogenetic gene synteny analysis we identified an angiosperm PIN clade sister PIN1, here...
In Arabidopsis, development during flowering is coordinated by transport of the hormone auxin mediated polar-localized PIN-FORMED1 (AtPIN1). However Arabidopsis has lost a PIN clade sister to AtPIN1, Sister-of-PIN1 (SoPIN1), which conserved in plants. We previously proposed that AtPIN1 organ initiation and vein patterning functions are split between SoPIN1 PIN1 clades grasses. Here we show grass Brachypodium sopin1 mutants have defects similar atpin1, while loss function little effect on but...
In Agave tequilana, reproductive failure or inadequate flower development stimulates the formation of vegetative bulbils at bracteoles, ensuring survival in a hostile environment. Little is known about signals that trigger this probably unique phenomenon agave species. Here we report auxin plays central role bulbil and show localization PIN1-related proteins consistent with altered transport during process. Analysis transcriptome data led to identification A. tequilana orthologue PIN1...
Lipo-chitooligosaccharides (LCOs) are microbial symbiotic signals that also influence root growth. In Medicago truncatula, LCOs stimulate lateral formation (LRF) synergistically with auxin. However, the molecular mechanisms of this phenomenon and whether it is restricted to legume plants not known. We have addressed capacity model monocot Brachypodium distachyon (Brachypodium) respond auxin for LRF. For this, we used a combination phenotyping assays, live-imaging quantification, analysed...
Barley (Hordeum vulgare) is an important global cereal crop and a model in genetic studies. Despite advances characterising barley genomic resources, few mutant studies have identified genes controlling root architecture anatomy, which plays critical role capturing soil resources. Our phenotypic screening of TILLING collection line TM5992 exhibiting short-root phenotype compared with wild-type (WT) Morex background. Outcrossing variety Proctor subsequent SNP array-based bulk segregant...
Leaf development presents a tremendous resource for tackling the question of patterning in biology. Leaves can be simple or highly dissected. They may have elaborated parts such as tendrils pea leaf rolled blade carnivorous pitcher plant. Despite variation size, shape, and function, all leaves initiate same manner: from flanks meristem. The maize is useful analysis due to wealth mutants distinct tissues along proximal distal axis. distal, sheath proximal, ligule forms at blade/sheath...
ABSTRACT The root system of plants is a vital part for successful development and adaptation to different soil types environments. A major determinant the shape plant formation lateral roots, allowing expansion system. Arabidopsis thaliana, with its simple anatomy, has been extensively studied reveal genetic program underlying branching. However, get more general understanding development, comparative studies in species complex anatomy are required. Here, by combining optimized clearing...
Abstract The root system of plants is a vital part for successful development and adaptation to different soil types environments. Besides allowing exploration the water nutrients, it also provides anchorage. A major determinant shape plant formation lateral roots, expansion system. Arabidopsis thaliana , with its simple anatomy, has been extensively studied reveal genetic program underlying branching. However, get more general understanding development, comparative studies in species...
Replicated trait evolution can provide insights into the mechanisms underlying of biodiversity. One example replicated is awn, an organ elaboration in grass inflorescences. Awns are likely homologous to leaf blades. We hypothesized that awns have evolved repeatedly because a conserved blade developmental program continuously activated and suppressed over course evolution, leading repeated emergence loss awns. To evaluate predictions arising from our hypothesis, we used ancestral state...
Replicated trait evolution can provide insights into the mechanisms underlying of biodiversity. One example replicated is awn, an organ elaboration in grass inflorescences. Awns are likely homologous to leaf blades. We hypothesized that awns have evolved repeatedly because a conserved blade developmental program continuously activated and suppressed over course evolution, leading repeated emergence loss awns. To evaluate predictions arising from our hypothesis, we used ancestral state...
Live confocal imaging of fluorescent reporters and stains in plant meristems provides valuable measurements gene expression, protein dynamics, cell polarity, division, growth. The spikelet meristem the grass Brachypodium distachyon (Brachypodium) is well suited to live because ease dissection, small size, simple arrangement organs, each abundant meristems. also far easier genetically transform than other species. Presented here a protocol for growth, staging, mounting, imaging.
Abstract In Arabidopsis, development during flowering is coordinated by transport of the hormone auxin mediated polar-localized PIN-FORMED1 (AtPIN1). However Arabidopsis has lost a PIN clade sister to AtPIN1, Sister-of-PIN1 (SoPIN1), which conserved in plants. We previously proposed that AtPIN1 organ initiation and vein patterning functions are split between SoPIN1 PIN1 clades grasses. Here we show grass Brachypodium sopin1 mutants have defects similar atpin1 , while loss function little...
Conference Abstract| October 01 2001 ONECUT domain transcription factor in the zebrafish D. O'Connor; O'Connor 1Dept. of Biochemistry, National University Ireland, galway, Ireland. Search for other works by this author on: This Site PubMed Google Scholar L. Byrnes Author and article information Publisher: Portland Press Ltd Online ISSN: 1470-8752 Print 0300-5127 © Biochemical Society2001 Biochem Soc Trans (2001) 29 (5): A110. https://doi.org/10.1042/bst029a110a Views Icon Article contents...