A5008030692- Plant Reproductive Biology
- Plant Molecular Biology Research
- Plant Physiology and Cultivation Studies
- Plant and animal studies
- Horticultural and Viticultural Research
- Plant-Microbe Interactions and Immunity
- Plant Virus Research Studies
- Fungal Plant Pathogen Control
- Plant biochemistry and biosynthesis
- Plant Pathogens and Fungal Diseases
- Plant Stress Responses and Tolerance
- Plant tissue culture and regeneration
- Plant and Fungal Interactions Research
- Plant nutrient uptake and metabolism
- Postharvest Quality and Shelf Life Management
- Insect-Plant Interactions and Control
- Berry genetics and cultivation research
- Plant Gene Expression Analysis
- Plant Micronutrient Interactions and Effects
- Photosynthetic Processes and Mechanisms
- Legume Nitrogen Fixing Symbiosis
- Plant Disease Management Techniques
- Plant Pathogenic Bacteria Studies
- Fermentation and Sensory Analysis
- Plant Disease Resistance and Genetics
China Agricultural University
2016-2025
Henan University of Science and Technology
2012
Xinjiang Agricultural University
2010
Hirosaki University
2007-2009
Institute of Pomology
2009
Aomori Prefectural Industrial Technology Center
2009
Jingdong (China)
2008
Abstract Varieties of the European pear (Pyrus communis) can produce trees with both red- and green-skinned fruits, such as Max Red Bartlett (MRB) variety, although little is known about mechanism behind this differential pigmentation. In study, we investigated pigmentation MRB its sport (MRB-G). The results suggest that a reduction in anthocyanin concentration causes MRB-G sport. Transcript levels PcUFGT (for UDP-glucose:flavonoid 3-O-glucosyltransferase), key structural gene biosynthesis,...
Abstract Expression of MdACS1, coding for 1-aminocyclopropane-1-carboxylate synthase (ACS), parallels the level ethylene production in ripening apple (Malus domestica) fruit. Here we show that expression another ripening-specific ACS gene (MdACS3) precedes initiation MdACS1 by approximately 3 weeks; MdACS3 then gradually decreases as increases. Because continues fruit treated with 1-methylcyclopropene, its transcription appears to be regulated a negative feedback mechanism. Three genes...
MicroRNAs (miRNAs) are key regulators of gene expression that post-transcriptionally regulate transcription factors involved in plant physiological activities. Little is known about the effects miRNAs disease resistance apple (Malus×domestica). We globally profiled cultivar Golden Delicious (GD) infected or not with leaf spot fungus Alternaria alternaria f. sp. mali (ALT1), and identified 58 exhibited more than a 2-fold upregulation upon ALT1 infection. pair target protein-coding genes...
Transcriptional gene silencing (TGS) can be induced by promoter-targeted small interfering RNA (siRNA). Long-distance transmission of TGS viral infection in plants has been reported. However, systemic not observed the case using an inverted repeat transgene as trigger. Here it is reported that a mobile signal, presumably siRNA, produced from hairpin structure controlled companion cell-specific promoter also induce transmissible both modified agroinfiltration and grafting system. Although...
Summary Self‐incompatibility ( SI ) is a reproductive isolation mechanism in flowering plants. Plants the S olanaceae, R osaceae and P lantaginaceae belong to gametophytic self‐incompatibility type. ‐ RN ase, which encoded by female‐specific gene located at locus, degrades RNA pollen tube causes . Recent studies have provided evidence that ase transported non‐selectively into tube, but not specified how this transportation accomplished. We show here apple M alus domestica MdABCF protein,...
Apple leaf spot caused by the Alternaria alternata f. sp mali (ALT1) fungus is one of most devastating diseases apple (Malus × domestica). We identified a hairpin RNA (hpRNA) named MdhpRNA277 that produces small RNAs and induced ALT1 infection in 'Golden Delicious' apple. mdm-siR277-1 mdm-siR277-2, which target five resistance (R) genes are expressed at high levels resistant variety 'Hanfu' low susceptible following infection. was strongly but not inoculation. promoter activity much stronger...
Alternaria leaf spot seriously threatens the sustainable development of global apple industry, causing significant losses and reducing fruit quality yield. The causal agent alternata f. sp. mali (Alternaria mali, ALT) produces various molecules to modulate infection, such as cell wall-degrading enzymes, toxins, elicitor-like molecules. ALT host-specific AM-toxin, an important pathogenicity factor. also releases effectors into cells that modify host defense, but these proteins have not yet...
Iron deficiency is one of the most common nutritional disorders in plants, especially fruit trees grown calcareous soil. Malus baccata widely used as an apple rootstock north China and highly resistant to low temperatures. There are few studies on iron absorption by this species at molecular level. It very important understand mechanism uptake transport such woody plants. As a helpful tool, aim present study was cloning functional analysis NRAMP (natural resistance-associated macrophage...
As a core factor in S-RNase-based gametophytic self-incompatibility (GSI), the SCF (SKP1–Cullin1–F-box-Rbx1) complex (including pollen determinant SLF, S-locus-F-box) functions as an E3 ubiquitin ligase on non-self S-RNase. The is formed by SKP1 bridging between CUL1, and Rbx1; however, it not known whether lacking can mediate ubiquitination of Three SKP1-like genes from were cloned based structural features SLF-interacting-SKP1-like (SSK) gene 'Golden Delicious' apple genome. These have...
In plants, post-transcriptional gene silencing (PTGS) spreads systemically, being transmitted from the silenced stock to scion expressing corresponding transgene. It has been reported that a graft-transmitted siRNA signal can also induce PTGS of an endogenous gene, but this was done by top-grafting using stock. present study involving grafting Nicotiana benthamiana, we found glutamate-1-semialdehyde aminotransferase (GSA), which acts as visible marker RNAi via inhibition chlorophyll...
Glomerella leaf spot (GLS) of apple (Malus×domestica Borkh.), caused by cingulata, is an emerging fungal epidemic threatening the industry. Little known about molecular mechanism underlying resistance to this devastating fungus. In study, high-throughput sequencing technology was used identify microRNAs (miRNAs) involved in GLS apple. We focused on miRNAs that target genes related disease and found expression a novel miRNA, Md-miRln20, higher susceptible varieties than resistant ones....
Distant hybridization, such as between apple (Malus domestica Borkh.) and pear (Pyrus communis L.), can introduce unique genetic traits that aren’t possible through traditional hybridization methods. However, these hybrids often display maternal traits, making it difficult to visually identify hybrid offspring in the early stages. Thus, identifying has been a longstanding challenge. To address this challenge, study performed whole-genome resequencing of ‘Golden Delicious’ ‘Yan Zhuang’, along...
Abstract Pears rank as the third most produced fruit in China, with widespread cultivation throughout country. Asexual propagation, primarily through grafting onto Pyrus betulifolia rootstocks, is predominant method of cultivation. However, absence an efficient genetic transformation system for P. significantly hinders enhancement efforts pear rootstocks. In this study, TDL39, a genotype, was identified its remarkable regenerative capacity. Leaf strips derived from 60-day-old TDL39 seedlings...
Grafting is widely used as a breeding method to enhance productivity and resilience. However, the mechanisms of graft healing remain poorly understood. In this study, we performed Malus domestica (‘Hanfu’) homograft observed morphological anatomical changes during process in junction within 40 days after grafting (DAG) with focus on vascular connection between stock scion traced acid fuchsin dye. The observation showed different phases process. Phase I, from 0 20 DAG,...
Summary Little is known about the mechanisms by which mRNA s are transported over long distances in phloem between rootstock and scion grafted woody plants. We identified an pear variety ‘Du Li’ ( Pyrus betulaefolia ) that was shown to be transportable phloem. It contains a WUSCHEL ‐ RELATED HOMEOBOX WOX domain therefore named Wox Transport 1 PbWoxT1 ). A 548‐bp fragment of critical long‐distance transport. rich CUCU polypyrimidine domains its interact with tract binding protein, Pb PTB 3....
S-RNase is the female determinant of gametophytic self-incompatibility in apple and usually considered to be reason for rejection pollen. In this study, we investigated role microtubules (MTs) internalization S-RNases by pollen tubes cultured vitro. The results showed that was imported into tube where it inhibits growth, co-localized with Golgi vesicle during process. Moreover, MT depolymerization observed following accumulation cytosol. On other hand, prevented from entering when treated...