After a spinal cord injury, axonal regeneration over long distances is challenging due to the lack of physical guidance cues and bioactive signals. In this study, multichannel silk fibroin nanofiber conduit was fabricated improve injury repair by enhancing regeneration. The composed longitudinally oriented nanofibers then functionalized with laminin. vitro, conduits could promote neuron-like development directional neurite extension PC12 cells providing stimulus guidance. model in...

The cationized <italic>Bombyx mori</italic> silk fibroin as a delivery carrier of the VEGF165–Ang-1 coexpression plasmid promoted neovascularization and dermal regeneration.

Ti3SiC2-based composites prepared with elemental powders exhibit advantages such as thermodynamic stability and high bonding strength. In ternary systems, data, including the phase equilibrium relationships of system, provide essential information for determining reaction mechanisms final products. The diagram Ti-Si-C system existing data estimated unknown properties calculated chemical potential each component. composite was by spark plasma sintering (SPS) Ti, Si, C through in-situ at 1573...

Abstract Bi/g-C 3 N 4 /WO photocatalyst for degradation of Rhodamine B (RhB) in wastewater was synthesized by urea, sodium tungstate dihydrate, and bismuth nitrate pentahydrate. XRD, SEM, PL, UV-Vis DRS, EIS were used to characterize the morphological, structural, optical, electrical properties materials. The efficiency RhB 96.03% after 30 min irradiation with visible light (70 Ŵ metal halide lamp), it almost completely degraded 40 irradiation. After four cycle experiments, 87%, indicating...

To engineer tissue restoration, it is necessary to provide a bioactive, mechanically robust scaffold. Electrospun poly(ε-caprolactone) (PCL) nanofiber promising biomaterial candidate with excellent mechanical properties, but PCL scaffolds are inert and lack natural cell recognition sites. overcome this problem we investigated the incorporation of Antheraea pernyi silk fibroin (ASF) containing inherent RGD tripeptides in electrospinning process. The mixing ratios showed remarkable impact on...

Basic fibroblast growth factor (bFGF) plays a significant role in stimulating cell proliferation. It remains challenge the field of biomaterials to develop carrier with capacity continuously releasing bioactive bFGF. In this study, porous bFGF-loaded silk fibroin (SF) microspheres, inside-out channels, were fabricated by high-voltage electrostatic differentiation, and followed lyophilization. The embedded bFGF exhibited slow release mode for over 13 days without suffering burst release. SEM...

The goal of this proof-of-concept study was the fabrication porous silk fibroin (SF) microspheres which could be used as cell culture carriers under very mild processing conditions. SF solution differentiated into droplets were induced by a syringe needle in high-voltage electrostatic field. They collected and frozen liquid nitrogen water formed ice crystals sublimated during lyophilization great quantity micropores shaped microspheres. Finally, treated ethanol so to transfer molecular...

There is an increasing demand for crosslinking methods of silk fibroin (SF) scaffolds in biomedical applications that could maintain the biocompatibility, bioactivity as well improve water resistance and mechanical properties SF materials. In this study, was crosslinked effectively with genipin which a naturally occurring iridoid glucoside mechanism investigated through FTIR amino acid analysis. The results showed react -NH2 groups on side chains macromolecules to form inter- intra-molecular...

Cancer gene therapy requires both effective tumor suppressor genes and safe vectors that express target efficiently. Inhibitor of growth 4 (ING4) inhibits via multiple pathways. Interleukin-24 (IL-24) also has tumor-suppressive activity against a broad spectrum human cancers. Adenovirus (Ad) exhibit high infection efficiency, but potential toxicity related to doses adenovirus led careful reconsideration their use in clinical trials. Antheraea pernyi silk fibroin (ASF) is cytocompatible...

Dermal graft with rapid vascularization was developed by integrating a biomimetic silk fibroin scaffold and Wharton's jelly MSC-derived endothelial cells.

Vascularization remains a critical challenge in dermal tissue regeneration. In this study, vascular endothelial growth factor (VEGF165) and angiopoietin-1 (Ang-1) dual gene coexpression vector that encoded green fluorescent protein (GFP) was constructed from an arginine–glycine–aspartic acid-modified adenovirus. Silk fibroin (SF) scaffolds loaded with adenovirus vectors were fabricated by freeze-drying method. vitro, the human endothelial-derived cell line EA.hy926 infected then expressed...

Herein, highly transparent alkaline lignin (AL)-decorated, environmentally friendly, water-resistant waterborne polyurethane (WPU) antibacterial composite films (WPU/AL-MC) were constructed by incorporation of 1-chloro-2,2,5,5-tetramethyl-4-imidazolinone (MC) N-halamine biocide via a facile solution-casting technique. Interestingly, as-synthesized WPU/AL-MC showed reinforced mechanical properties (20.80 MPa) and high-efficiency ultraviolet (UV)-shielding performance (over 85% the UVA...

In order to investigate the effects of addition amount, particle size distribution and aver- age NaCl porogen on pore connectivity silk fibroin (SF) / hydroxyapatite (HA) porous composites. this paper, SF/HA composites were fabricated by means isostatic pressing. The structure was observed Scanning electron microscopy (SEM), air permeability tested home-made device. Results indicated that larger amount particles, greater scope particles increased average able make material improved...

Angiogenesis of an implanted tissue is one the most important issues in skin repairment. Porous bFGF-immobilized silk fibroin (bSF) scaffolds was fabricated by freeze drying using EDC agent crosslinking bFGF and SF with aim controlling delivery for revascularization improvement. The bSF retained a porous structure 200～300 μm diameter 85～91% porosity. seeded L929 cells had notably improved growth proliferation on scaffolds, suggesting sustained release from remained bioactivity. After...

The composite tubular scaffolds with highly oriented nanofibers used for vascular repair could improve the biomechanical properties of scaffolds, satisfying requirement change blood pressure and conducing to cell adhesion, migration proliferation. from poly(ε-caprolactone)(PCL) silk fibroin (SF) were successfully fabricated through electrospinning using cylindrical roller an outer diameter (OD) 3.0 mm. influences collecting rotatation speeds on electrospun PCL/SF orientation radial/axial...