A5100417241- Bladder and Urothelial Cancer Treatments
- Fluoride Effects and Removal
- Cancer Immunotherapy and Biomarkers
- Epigenetics and DNA Methylation
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Bone and Dental Protein Studies
- Immunotherapy and Immune Responses
- Cancer Genomics and Diagnostics
- Silk-based biomaterials and applications
- Ferroptosis and cancer prognosis
- Bone Metabolism and Diseases
- Immune cells in cancer
- Endometriosis Research and Treatment
- Ubiquitin and proteasome pathways
- Urinary and Genital Oncology Studies
- Acute Ischemic Stroke Management
- Hedgehog Signaling Pathway Studies
- Cancer, Hypoxia, and Metabolism
- Antiplatelet Therapy and Cardiovascular Diseases
- Cancer-related Molecular Pathways
- Electrospun Nanofibers in Biomedical Applications
- Bone Tissue Engineering Materials
- Health Systems, Economic Evaluations, Quality of Life
- Cancer-related gene regulation
- RNA Interference and Gene Delivery
Northwestern University
2014-2025
Boehringer Ingelheim (United States)
2010-2025
Shenzhen University
2024-2025
First People's Hospital of Nanning
2024
First Affiliated Hospital Zhejiang University
2024
Guiyang Medical University
2013-2023
Northwestern University
2021
Affiliated Hospital of Guizhou Medical University
2020-2021
Soochow University
2014-2019
Zhejiang University
2019
Abstract Checkpoint immunotherapy (CPI) has increased survival for some patients with advanced-stage bladder cancer (BCa). However, most do not respond. Here, we characterized the tumor and immune microenvironment in pre- post-treatment tumors from PURE01 neoadjuvant pembrolizumab trial, using a consolidative approach that combined transcriptional genetic profiling digital spatial profiling. We identify five distinctive transcriptomic programs validate these an independent CPI trial to...
The N-butyl-N-(4-hydroxybutyl)-nitrosamine (BBN) mouse model is an attractive system of muscle-invasive bladder cancer (MIBC) as it recapitulates the histology human tumors in a background with intact immune system. However, was unknown whether this carcinogen-induced also mimicked MIBC at molecular and mutational level. In our study, we analyzed gene expression landscape BBN by next-generation sequencing followed bioinformatic comparison to using data from Cancer Genome Atlas other...
// Alexander P. Glaser 1, 2 , Damiano Fantini Yiduo Wang Yanni Yu Kalen J. Rimar Joseph R. Podojil 3 Stephen D. Miller and Joshua Meeks 1 Department of Urology, Northwestern University, Feinberg School Medicine, Chicago, IL, USA Robert H. Lurie Comprehensive Cancer Center, Interdepartmental Immunobiology Microbiology-Immunology, Correspondence to: Meeks, email: Joshua.meeks@northwestern.edu Keywords: urinary bladder neoplasms; APOBEC deaminases; mutagenesis; DNA damage; interferon Received:...
Various organogel materials with either a liquid or solid surface layer have recently been designed and prepared. In this work, amphiphilic organogels (AmOG) are innovatively developed from copolymer P(PDMS-r-PEG-r-GMA) 2,2'-diaminodiphenyldisulfide via epoxy group addition reaction then infiltrated lubricants instead of traditional hydrophilic hydrophobic lubricants. Because synergistic effects segments lubricants, the AmOG surfaces showed high stability excellent anti-icing performance....
The increasing prevalence of food allergy (FA) is a growing clinical and public health problem. contribution genetic factors to FA remains largely unknown.This study examined the pattern familial aggregation degree which contribute sensitization allergens.This included 581 nuclear families (2,004 subjects) as part an ongoing in Chicago, IL, USA. was defined by set criteria including timing, symptoms obtained via standardized questionnaire interview corroborative specific IgE cut-offs for >...
The pathogenesis of endometriosis remains unclear, and relatively little is known about the mechanisms that promote establishment survival disease. Previously, we demonstrated v-akt murine thymoma viral oncogene homolog (AKT) activity was increased in tissues cells from ovarian endometriomas this increase promoted cell as well decreased levels progesterone receptor. objective study to demonstrate a role for AKT ectopic lesions. First, dose-dependent inhibition stromal human (OSIS)...
Idiopathic pulmonary fibrosis (IPF) is a rare and serious disease characterized by progressive lung-function loss. Limited evidence has been published on the impact of loss subsequent patient outcomes. This study examined change in forced vital capacity (FVC) across IPF patients 6 months after diagnosis its association with clinical healthcare resource utilization (HRU) outcomes real-world setting U.S. A retrospective chart review was conducted diagnosed pulmonologists. Patient eligibility...
The long-term survival of patients with advanced urothelial carcinoma (UCa) is limited because innate resistance to treatment. We identified elevated expression the histone methyltransferase EZH2 as a hallmark aggressive UCa and hypothesized that inhibition, via small-molecule catalytic inhibitor, might have antitumor effects in UCa. Here, carcinogen-induced mouse bladder cancer model, reduction tumor progression an increase immune infiltration upon inhibition were observed. Treatment mice...
This study examined whether the prevention of diabetes-related albuminuria by aminoguanidine (AG) or ramipril (RAM) may be mediated a common post-glomerular basement membrane renal intracellular mechanism involving protein kinase C (PKC). The handling albumin was over 24 weeks in control and streptozotocin (STZ)-induced diabetic rats. A radioimmunoassay (RIA) that measures intact albumin, intravenously injected tritium-labeled rat serum used to assess proportion fragments urine. Diabetes...
DNA topoisomerase (topo) I is an essential nuclear protein and a target for anticancer drug camptothecin derivatives. As protein, topo concentrated in the nucleolus. However, this nucleolar distribution of dynamic. It has been shown recently that rapidly moves out nucleolus (nucleolar delocalization) response to inhibitors. In present study, we demonstrated delocalization associated with its conjugation by SUMOs (small ubiquitin-like modifiers) inhibitor topotecan. Time-course experiments...
The transient receptor potential canonical (TRPC) family channels are proposed to be essential for store-operated Ca2+ entry in endothelial cells. signaling is involved NF-κB activation, but the role of unclear. Here we show that thrombin-induced and resultant AMP-activated protein kinase (AMPK) activation targets Ca2+-independent Cδ (PKCδ) mediate We observed p65/RelA, AMPK, PKCδ were markedly reduced by knockdown TRPC isoform TRPC1 expressed human cells obtained from Trpc4 knock-out mice....
Abstract Cancer cells accumulate somatic mutations as result of DNA damage, inaccurate repair and other mechanisms. Different genetic instability processes in characteristic non-random patterns mutations, also known mutational signatures. We developed mutSignatures , an integrated R-based computational framework aimed at deciphering Our software provides advanced functions for importing variants, computing mutation types, extracting signatures via non-negative matrix factorization....
BACKGROUND: Idiopathic pulmonary fibrosis (IPF) is a rare and fatal restrictive respiratory disease under the idiopathic lung (ILD) class. IPF form of chronic, progressive fibrosing interstitial pneumonia has more scarring, less inflammation, poorer prognosis than most other ILD forms. Exacerbation rapid, with unpredictable deterioration function, associated short-term mortality. The American Thoracic Society (ATS) evidence-based guidelines for diagnosis management reports that incidence...
Abstract Ubc9 is an E2-conjugating enzyme required for sumoylation and has been implicated in regulating several critical cellular pathways. We have shown previously that important nucleolar delocalization of topoisomerase (topo) I response to topo inhibitors such as topotecan. However, the role tumor drug responsiveness not clear. In this study, we found although MCF7 cells expressing a dominant-negative mutant (Ubc9-DN) display decreased activity I, these are more sensitive inhibitor...
Thrombin activation of protease-activated receptor-1 induces Ca(2+) influx through store-operated cation channel TRPC1 in endothelial cells. We examined the role induced by depletion stores signaling expression Both thrombin and a receptor-1-specific agonist peptide human umbilical vein cells, which was coupled to an augmented increase permeability. To delineate mechanisms thrombin-induced expression, we transfected cells TRPC1-promoter-luciferase (TRPC1-Pro-Luc) construct containing...
The endocervix has both anatomical and biological functions that participate in the delicate balance between tolerance necessary for conception protection from pathogens. Our goal was to develop a robust 3-dimensional (3D) model reliable representation of vivo tissues identify physiological responses changing levels steroid hormones during 28-day time period. Human endocervical cells were grown on polystyrene scaffolds, morphologic hormonal cultured assessed response fluctuating estradiol...
Patients with locally advanced and metastatic urothelial carcinoma have a low survival rate (median 15.7 months, 13.1–17.8), only 23% response to monotherapy treatment anti-PDL1 checkpoint immunotherapy. To identify new therapeutic targets, we profiled the immune regulatory signatures during murine cancer development using BBN carcinogen identified an increase in expression of T cell inhibitory protein B7-H4 (VTCN1, B7S1, B7X). temporally correlated decreased lymphocyte infiltration. While...