A5024959188- MicroRNA in disease regulation
- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- Genetic factors in colorectal cancer
- RNA Research and Splicing
- Cancer-related molecular mechanisms research
- Gut microbiota and health
- Cancer Genomics and Diagnostics
- RNA modifications and cancer
- Microbial Metabolic Engineering and Bioproduction
- Reproductive Physiology in Livestock
- Probiotics and Fermented Foods
- Lipid metabolism and biosynthesis
- Microbial bioremediation and biosurfactants
- Sports injuries and prevention
- Microbial Community Ecology and Physiology
- Reproductive System and Pregnancy
- Exercise and Physiological Responses
Oslo University Hospital
2019-2025
University of Oslo
2019-2025
Abstract The respiratory tract is colonized with low-density microbial communities, which have been shown to impact human health through microbiota–host interactions. However, a lack of fast and cost-effective nucleic acid extraction method for low-microbial biomass samples hinders investigation microbiota. Here, we performed pilot study assess the suitability NAxtra protocol profiling bacterial microbiota in samples. A small number nasopharyngeal aspirate ( n = 8), nasal swab saliva 8) were...
High-throughput sequencing is increasingly favoured to assay the presence and abundance of microRNAs (miRNAs) in biological samples, even from low RNA amounts, a number commercial vendors now offer kits that allow miRNA sub-nanogram (ng) inputs. Although biases introduced during library preparation have been documented, relative performance current reagent has not investigated detail. Here, six capable handling <100ng total input were used for preparation, performed by kit manufactures, on...
ABSTRACT The human skin microbiome is crucial for health and immunity, especially under the extreme conditions military personnel face. Soldiers often encounter unique stressors hygienic challenges that can alter their skin’s microbial composition, particularly in field environments. In this study, we aimed to investigate impact of exercises on diversity composition bacterial microbiota using 16S rRNA sequencing. We conducted a longitudinal study Norwegian soldiers ( n = 19) participating...
Abstract Lynch Syndrome (LS) is a hereditary cancer syndrome caused by pathogenic germline variants in one of the four mismatch repair (MMR) genes MLH1 , MSH2 MSH6 and PMS2 . It characterized significantly increased risk multiple types, particularly colorectal endometrial cancer, with autosomal dominant inheritance. Access to precise sensitive methods for genetic testing important, as early detection prevention possible when variant known. We present here two unrelated Norwegian families...
ABSTRACT High-throughput sequencing has emerged as the favoured method to study microRNA (miRNA) expression, but biases introduced during library preparation have been reported. We recently compared performance (sensitivity, reliability, titration response and differential expression) of six commercially-available kits on synthetic miRNAs human RNA, where was performed by vendors. hereby supplement this with data from two further commonly used (NEBNext, NEXTflex) whose manufacturers...
Abstract The human skin microbiome is crucial for health and immunity, especially in the context of extreme conditions faced by military personnel. Soldiers encounter unique stressors hygienic challenges that can impact microbial composition their during field exercises regular occupational settings. In this study, we aimed to investigate a exercise on diversity bacterial microbiota using 16S rRNA sequencing. We conducted longitudinal study NATO Cold Response 2022 , involving Norwegian...
Abstract High-throughput sequencing is increasingly favoured to assay the presence and abundance of micro RNAs (miRNAs) in biological samples, even from low RNA amounts, a number commercial vendors now offer kits that allow miRNA sub-nanogram (ng) inputs. However, although biases introduced during library preparation have been documented, relative performance current reagent has not investigated detail. Here, six capable handling <100ng total input were used for preparation, performed by...
Abstract High-throughput sequencing has emerged as the favoured method to study microRNA (miRNA) expression, but biases introduced during library preparation have been reported. To assist researchers choose most appropriate kit, we recently compared performance of six commercially-available kits on synthetic miRNAs and human RNA, where was performed by vendors. We hereby supplement this with data from two further commonly used (NEBNext, NEXTflex) whose manufacturers initially declined...
Abstract Lynch Syndrome (LS) is a hereditary cancer syndrome caused by pathogenic germline variants in one of the four mismatch repair (MMR) genes MLH1 , MSH2 MSH6 and PMS2 . It characterized significantly increased risk multiple types, particularly colorectal endometrial cancer, with autosomal dominant inheritance. Access to precise sensitive methods for genetic testing important, as early detection prevention possible when variant known. We present here two unrelated Norwegian families...