A5001526364- Virus-based gene therapy research
- Viral Infections and Outbreaks Research
- Diabetes Management and Research
- Virology and Viral Diseases
- Respiratory viral infections research
- Viral gastroenteritis research and epidemiology
- Pancreatic function and diabetes
- Viral Infections and Vectors
- COVID-19 Clinical Research Studies
- Hepatitis B Virus Studies
- Intensive Care Unit Cognitive Disorders
- Immune cells in cancer
- Sepsis Diagnosis and Treatment
- Analytical Chemistry and Chromatography
- Pneumonia and Respiratory Infections
- Lymphoma Diagnosis and Treatment
- Diabetes Management and Education
- Genetically Modified Organisms Research
- Tracheal and airway disorders
- Trypanosoma species research and implications
- Artificial Intelligence in Healthcare and Education
- COVID-19 epidemiological studies
- Trauma and Emergency Care Studies
- Metabolism and Genetic Disorders
- Urinary Tract Infections Management
Hospital Riotinto
2021-2024
Hospital Universitario Virgen Macarena
2019-2023
University of Amsterdam
2023
Lillebaelt Hospital
2023
University of Southern Denmark
2023
Ankara Bilkent City Hospital
2023
University of Oslo
2023
Vrije Universiteit Amsterdam
2023
Amsterdam University Medical Centers
2023
Oslo University Hospital
2023
BACKGROUND-AIMThe Jaffe method tests for creatinine based on the formation of a colored complex between and alkaline picrate but interfering substances can cause errors.The enzymatic reduces interferences not all labs adopted it due to costs.This study aims create statistical model correct produce results similar those from method, make an easy way adopt daily use. METHODSThree hundred thirty-three (333) patients were enrolled March 20 29, 2019, including walk-in in-patients.Serum values...
ABSTRACT Respiratory syncytial virus (RSV), a member of the Paramyxoviridae family, encodes small hydrophobic (SH) protein unknown function. Parainfluenza 5 (PIV5), prototypical paramyxovirus, also an SH protein, which inhibits tumor necrosis factor alpha (TNF-α) signaling. In this study, recombinant PIV5 viruses without their own but containing RSV (from strain A2 or B1) in its place (PIV5ΔSH-RSV SH) and lacking (RSVΔSH) were generated analyzed. The results indicate that has function...
Respiratory Syncytial Virus (RSV) is the major cause of pneumonia among infants. Here we elucidated antibody repertoire following primary RSV infection and traced its evolution through adolescence adulthood. Whole genome-fragment phage display libraries (GFPDL) expressing linear conformational epitopes in fusion protein (F) attachment (G) were used for unbiased epitope profiling infant sera prior to infection. F-GFPDL analyses demonstrated modest changes anti-F repertoires post-RSV...
Abstract Polyclonal antibodies, derived from humans or hyperimmunized animals, have been used prophylactically therapeutically as countermeasures for a variety of infectious diseases. SAB Biotherapeutics has successfully developed transchromosomic (Tc) bovine platform technology that can produce fully human immunoglobulins rapidly, and in substantial quantities, against disease targets. In this study, two Tc bovines expressing high levels IgG were with recombinant glycoprotein (GP) vaccine...
Epidemics and pandemics have been shown to widespread effects on health systems. Diabetes is a condition of particular risk during national emergencies such as the COVID-19 pandemic. The aim this study determine influence in patient's diabetes quality management. We conducted retrospective multicenter cohort study. Data from type 2 patients living Sevilla were included into was divided two observation periods, before COVID-19. Metabolic status assessed using levels hemoglobin A1c (A1C)...
Resumen Objetivos La predicción temprana de enfermedad crítica por COVID-19 es crucial para optimizar el manejo clínico. Este estudio tiene como objetivo la mediante integración datos clínicos, laboratorio y polimorfismos genéticos en modelos inteligencia artificial, evaluando comparando rendimiento distintos algoritmos aprendizaje automático. Métodos Se analizaron 155 pacientes hospitalizados, 23 los cuales desarrollaron crítica. realizó un análisis univariante evaluar asociación entre...
Mumps virus (MuV) causes an acute infection in humans characterized by a wide array of symptoms ranging from relatively mild manifestations, such as parotitis, to more-severe complications, meningitis and encephalitis. Widespread mumps vaccination has reduced incidence dramatically; however, outbreaks still occur vaccinated populations. The V protein MuV, when expressed cell culture, blocks interferon (IFN) expression signaling interleukin-6 (IL-6) signaling. In this work, we generated...
At 18,954 nucleotides, the J paramyxovirus (JPV) genome is one of largest in family Paramyxoviridae, consisting eight genes order 3'-N-P/V/C-M-F-SH-TM-G-L-5'. To study function novel JPV, a plasmid containing full-length cDNA clone JPV was constructed. In this study, small hydrophobic (SH) protein examined by generating recombinant lacking coding sequence SH (rJPVΔSH). rJPVΔSH viable and had no growth defect tissue culture cells. However, more tumor necrosis factor alpha (TNF-α) produced...
Infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes a disease (coronavirus 2019, COVID-19) that may develop into systemic immunosuppression and death in its form. Myeloid-derived suppressive cells (MDSCs) are inhibitory contribute to patients cancer infection. Increased levels of MDSCs have been found COVID-19 patients, although their role the pathogenesis has not clarified. For this reason, we raised question whether could be useful follow-up intensive care...
Significance J paramyxovirus represents a group of unclassified paramyxoviruses that encode an integral membrane protein, transmembrane (TM), is not found in other classified paramyxoviruses. In this work we have TM expression involved cell-to-cell fusion when coexpressed with the viral protein F or and receptor-binding G. This may define separate class proteins promote fusion.
Limited knowledge exists on the quality of polyclonal antibody response generated following Ebola virus (EBOV) infection compared with vaccination. Polyclonal repertoire in plasma EBOV survivors was ChAd3-MVA prime-boost human Higher binding and affinity to GP observed vaccinated that correlated neutralization. Surprisingly, a predominant IgM after vaccination, whereas demonstrated IgG-dominant response. induced more diverse epitope A strong antigenic sites fusion peptide another highly...
Several Ebola vaccines and therapeutics are under clinical development. However, limited knowledge exists on the quality of antibody response generated by different vaccines. In this study, repertoire induced vaccination transchromosomal bovine (TcB) with virus (EBOV) glycoprotein ([GP]; recombinant GP [rGP]) encoded either deoxyribonucleic acid (DNA) or nanoparticle-based vaccine platform was analyzed using EBOV genome fragment phage display library surface plasmon resonance (SPR)-based...
Background Early identification of patients with COVID-19 who may develop critical illness is great importance.
Abstract Background The diagnosis of systemic autoimmune rheumatic diseases (SARD) is based on the detection serum antinuclear antibodies (ANA) for which indirect immunofluorescence (IIF) golden standard. New solid‐phase immunoassays have been developed to be used alone or in combination with extractable (ENA) improve SARD diagnosis. purpose this study was compare clinical performances different ANA screening methods ENA Methods A total 323 patients were screened by IIF, EliA™ CTD Screen,...
Abstract Objectives To describe a variant hemoglobin that interferes with HbA 1c analysis by cation exchange HPLC. Case presentation A 78 years-old Spanish male patient visited the Internal Medicine Clinic for routine check-up, included to screen diabetes. He had suffered hypertension and dyslipidemia, no previous symptoms suggestive of diabetes such as hyperglycemia, weight loss, polydipsia, polyuria or tiredness. Diabetes screening measurement was assessed using HPLC an immunoassay...
Resumen Objetivos Describir la variante de hemoglobina que interfiere en el análisis HbA 1c mediante HPLC intercambio catiónico. Caso clínico Paciente 78 años acude a consulta Medicina Interna para una revisión rutinaria, donde se incluye determinación detección diabetes. El paciente presenta como antecedentes hipertensión y dislipidemia sin presentar síntomas previos sugestivos diabetes hiperglucemia, pérdida peso, polidipsia, poliuria o cansancio. La prueba realizó con columna catiónico un...
Abstract Objective Zinc transporter 8 autoantibodies (ZNt8A) are 1 of the 4 main used for diagnosis type diabetes (T1D), with glutamic acid decarboxylase (GADA), islet antigen-2 (IA-2A), and insulin (IAA). The objective this study is to evaluate diagnostic efficiency these T1D in pediatric patients. Methods A retrospective analysis patients under 16 years age suspected was made between June 2020 January 2021. total 80 were included study, sample per patient. Subjects classified according...
SARS-CoV-2 infection is the cause of disease named COVID-19, a major public health challenge worldwide. Differences in severity, complications and outcomes COVID-19 are intriguing and, patients with similar baseline clinical conditions may have very different evolution. Myeloid-derived suppressor cells (MDSCs) been previously found to be recruited by marker evolution these patients. We studied 90 consecutive admitted hospital before vaccination program started general population, measure...
Background Human Respiratory Syncytial Virus (RSV) is a leading cause of pediatric pulmonary disease and severe RSV infection predisposes to wheezing later in life. has also been shown be an environmental trigger for asthma. We are investigating whether targeting host factors important viable antiviral strategy. Lowering viral burden through these therapies will result decreased severity may prevent the occurrence pathologic sequelae.