A5051635951- Nanoparticle-Based Drug Delivery
- 3D Printing in Biomedical Research
- Gold and Silver Nanoparticles Synthesis and Applications
- Diabetes Management and Research
- Advanced biosensing and bioanalysis techniques
- Pancreatic function and diabetes
- Nanoplatforms for cancer theranostics
- Microfluidic and Bio-sensing Technologies
- RNA Interference and Gene Delivery
- Diabetes and associated disorders
- Dendrimers and Hyperbranched Polymers
- Light effects on plants
- CRISPR and Genetic Engineering
- Cancer Cells and Metastasis
- Anatomy and Medical Technology
- Electrohydrodynamics and Fluid Dynamics
- Cell Image Analysis Techniques
- Erythrocyte Function and Pathophysiology
- Gene Regulatory Network Analysis
- Nanoparticles: synthesis and applications
- Advanced Fluorescence Microscopy Techniques
- Biosensors and Analytical Detection
University of Toronto
2014-2020
Toronto Public Health
2014-2016
Occupational Cancer Research Centre
2014
University of California, Irvine
2013
Edwards Lifesciences (United States)
2013
Coating the nanoparticle surface with cancer cell recognizing ligands is expected to facilitate specific delivery of nanoparticles diseased cells in vivo. While this targeting strategy appealing, no nanoparticle-based active formulation for solid tumor treatment had made it past phase III clinical trials. Here, we quantified cell-targeting efficiencies Trastuzumab (Herceptin) and folic acid coated gold silica multiple mouse models. Surprisingly, showed that less than 14 out 1 million...
Abstract Nanoparticles are commonly administered through systemic injection, which exposes them to the dynamic environment of bloodstream. Injected nanoparticles travel within blood and experience a wide range flow velocities that induce varying shear rates vessels. Endothelial cells line these vessels, have been shown uptake during circulation, but it is difficult characterize flow‐dependence this interaction in vivo. Here, microfluidic system developed control as they interact with...
The role of tissue architecture in mediating nanoparticle transport, targeting, and biological effects is unknown due to the lack tools for imaging nanomaterials whole organs. Here, we developed a rapid optical mapping technique image intact organs ex vivo three-dimensions (3D). We engineered high-throughput electrophoretic flow device simultaneously transform up 48 tissues into optically transparent structures, allowing subcellular more than 1 mm deep which 25-fold greater current...
Significance Structural analysis of microscale three-dimensional tissues (3D microtissues) in high-throughput is becoming increasingly important drug discovery, regenerative medicine, and other biomedical areas because they recapitulate many vivo biological features not present 2D models. This can be done by using microfluidic technology to control apply external forces on-chip 3D microtissues, imaging these organ-on-chip systems with confocal microscopy. However, the high cellular density...
Nanoparticles are engineered from materials such as metals, polymers, and different carbon allotropes that do not exist within the body. Exposure to these exogenous compounds raises concerns surrounding toxicity, inflammation, immune activation. These responses could potentially be mitigated by synthesizing nanoparticles directly molecules derived host. However, efforts assemble patient-derived macromolecules into structures with same degree of size shape tunability their counterparts...
Abstract The 3D architecture of blood vessel networks dictates how nutrients, waste, and drugs are transported. These transport processes difficult to study in vivo, leading researchers develop methods construct vitro. However, existing require expensive, customized equipment cannot create large (>1 cm 3 ) constructs. This makes them inaccessible many or educators. Here, a method that transcribes images into physical microfluidic devices is developed. takes uses fused‐filament fabrication...
A method to improve the genetic engineering of microtissues by increasing viral particle penetration using a microfluidic device.
Light-switchable proteins offer numerous opportunities as tools for manipulating biological systems with exceptional degrees of spatiotemporal control. Most designed light-switchable currently in use have not been optimised using the randomisation and selection/screening approaches that are widely used other areas protein engineering. Here we report an approach screening DNA-binding relies on light-dependent repression transcription a fluorescent reporter. We demonstrate method can be to...
Abstract Review: 99 refs.