ABSTRACT Survey results describing the levels and prevalences of zoonotic agents in 1,549 livestock waste samples were analyzed for significance with husbandry farm management practices. Statistical analyses survey data showed that groups containing calves <3 months age, piglets, or lambs had higher Campylobacter spp. Escherichia coli O157 their wastes. Younger still receiving milk, however, significantly lower prevalence E. O157. Furthermore, when wastes contained any form bedding, they...

To determine the prevalence and levels of zoonotic agents in livestock wastes.A proportionally weighted survey was undertaken waste samples analysed quantitatively for Escherichia coli O157, pathogenic Listeria, Salmonella, Campylobacter, Giardia Cryptosporidium. A significant proportion wastes contained at least one agent. Relationships were found between dry matter content presence some agents.British contain measurable that cause most cases gastroenteritis UK.Animal are disposed by...

ABSTRACT In response to reports that the contamination of food can occur during on-farm primary phase production, we report data describes a possible cost-effective intervention measure. The effect time before soil incorporation livestock wastes spread land on rate decline zoonotic agents present in waste was investigated. Fresh were inoculated with laboratory-cultured Salmonella , Listeria and Campylobacter spp. Escherichia coli O157 they spead onto soil. Incorporation either immediate,...

To determine the fate of Escherichia coli deposited onto grassland via faeces, from naturally colonized cattle, sheep or pigs.Groups and pigs were penned outdoors on grass during November, removed after 14 days. populations in ground declined over 134 days initial average levels 5.34, 4.31 4.96 log(10) CFU g(-1) pig pens, respectively. The maximum survival time was up to 162 (190 taking sampling interval deposition into account), but numbers varied significantly amongst 20 replicates taken...

Syringomycin is a necrosis-inducing lipopeptide toxin synthesized and secreted by the phytopathogen, Pseudomonas syringae pv. syringae. Although small quantities of syringomycin are known to activate cascade physiological events in plasma membranes, mechanism action phytotoxin has never been fully characterized. The objective this study was test hypothesis that primary mode form transmembrane pores permeable cations. Accordingly, direct measurement ion fluxes were performed using artificial...

To improve our understanding of the survival and splash-mediated transfer zoonotic agents faecal indicator bacteria introduced into soils used for crop cultivation via contaminated irrigation waters.Zoonotic an Escherichia coli marker bacterium were inoculated borehole water, which was applied to two different soil types in early-, mid- late summer. Decline influenced by type. Marker columns water did not concentrate at surface soils. type environmental conditions. Typically, declines rapid...

ABSTRACT This paper explores the intricacies of spatial probability sampling designs, with a specific focus on neglected topic comparing artificial areal unit support networks convenience existing political districts, and role autocorrelation (SA) in this interplay. The traditional approach when controlling for variable selection often involves creating customized units, such as regular hexagons forming tessellation. However, method introduces operational challenges, requiring significant...

To measure the decline rates of zoonotic agents introduced into liquid livestock wastes in on-farm storage tanks.Salmonella spp., Escherichia coli O157, Campylobacter jejuni, Listeria monocytogenes and Cryptosporidium parvum, propagated laboratory-controlled conditions, were inoculated 35,000-l volumes fresh (pig slurries, cattle slurries dirty waters). D-values for bacteria six to 44 days, C. parvum 133 345 days. jejuni declined significantly more rapidly than other bacterial pathogens,...

Journal Article The air‐borne distribution of zoonotic agents from livestock waste spreading and microbiological risk to fresh produce contaminated irrigation sources Get access M.L. Hutchison, Hutchison Division Farm Animal Science, Department Clinical Veterinary Bristol University, Langford, Bristol, UK Mike BS40 5DU, UK. E‐mail: mike.hutchison@bristol.ac.uk Search for other works by this author on: Oxford Academic Google Scholar S.M. Avery, Avery J.M. Monaghan Crops Department, Harper...

The aims of this study were (i) to determine the prevalence and numbers campylobacters in 63 samples raw livers purchased at retail across UK (ii) investigate whether freezing chicken contaminated with Campylobacter was a reliable method for decontamination. Chicken naturally subjected −15 −25°C one day 7 days. Numbers on determined immediately before after 24‐h or 7‐days freeze treatment daily during 3 days post‐thaw refrigerated storage. Freezing 24 h can reduce by up 2 log10 CFU g−1....

Aims: To measure the rates of decline zoonotic agents introduced into heaps spent bedding and faecal wastes generated by commercially farmed livestock managed in a similar way to that working farm. Methods Results: Livestock isolates Salmonella, pathogenic Listeria, Campylobacter Escherichia coli O157 were laboratory cultured used inoculate 5 m3 cattle, sheep or pig mixed with materials. Decline each infectious was monitored time as temperature inside heap. Temperatures >50°C typically...

Foodborne pathogens, such as Salmonella, may remain in abattoir lairages after cleansing and pose a risk of transfer contamination from one processing day to the next. These organisms be transferred outer surface animals held lairage facilities, skin or hide significant source microbial on red meat carcasses subsequently produced. Sponge samples were taken various sites (n = 556), single-pass sponge side 1,050) at five commercial abattoirs Southwest England tested for presence Salmonella. Of...

A study was undertaken to simulate the likely effects of a field worker with poor hygienic practices that had returned work too soon after recovering from an infection by enteric pathogen. The studies simulated variety hand-washing no washing soap and water followed application alcohol gel using latrine. numbers generic Escherichia coli isolated workers' hands declined increasing thoroughness treatments unwashed > water, gel. Where gloves were worn counts obtained for significantly reduced,...

Journal Article Thermal death of Escherichia coli O157:H7 in cattle feeds Get access M.L. Hutchison, Hutchison Research Division, DLS‐Eurofins Laboratories, Wolverhampton, UK HSS Ltd, Unit G, Mid Craigie Industrial Estate, Dundee, Mike Dundee DD4 7RH, Angus, UK. E‐mail: mh@mikehutchison.com Search for other works by this author on: Oxford Academic Google Scholar D.J.I. Thomas, Thomas S.M. Avery Letters Applied Microbiology, Volume 44, Issue 4, 1 April 2007, Pages 357–363,...

To identify production and processing practices that might reduce Campylobacter numbers contaminating chicken broiler carcasses. The of campylobacters were determined on carcass neck skins after or in house litter samples. Supplementary information described farm layouts, farming conditions for individual flocks, the slaughterhouse layouts operating inside plants was collected, matched with each test result. Statistical models predicting constructed. Carcass microbial contamination more...