A5060585718- Viral Infectious Diseases and Gene Expression in Insects
- Mycobacterium research and diagnosis
- Monoclonal and Polyclonal Antibodies Research
- Tuberculosis Research and Epidemiology
- CAR-T cell therapy research
- Cell Adhesion Molecules Research
- Protein purification and stability
- Immunotherapy and Immune Responses
- Pluripotent Stem Cells Research
- Smart Grid Energy Management
- Pneumocystis jirovecii pneumonia detection and treatment
- Energy Harvesting in Wireless Networks
- RNA Interference and Gene Delivery
- Hybrid Renewable Energy Systems
- Lysosomal Storage Disorders Research
- Bacteriophages and microbial interactions
- Genomics and Rare Diseases
- Biochemical and Molecular Research
- Infectious Diseases and Tuberculosis
- Virus-based gene therapy research
- CRISPR and Genetic Engineering
Centogene (Germany)
2022
Pasteur Institute of Iran
2016-2017
Biotechnology Research Center
2016-2017
Shiraz University of Medical Sciences
2011-2016
Abstract To present our experience using a multiomic approach, which integrates genetic and biochemical testing as first-line diagnostic tool for patients with inherited metabolic disorders (IMDs). A cohort of 3720 from 62 countries was tested panel including 206 genes single nucleotide copy number variant (SNV/CNV) detection, followed by semi-automatic filtering reflex (25 assays). In 1389 (37%), diagnosis achieved. Within this cohort, the highest yield obtained Asia (57.5%, mainly...
Establishing stable Chinese Hamster Ovary (CHO) cells producing monoclonal antibodies (mAbs) usually pass through the random integration of vectors to cell genome, which is sensitive gene silencing. One approach overcome this issue target a highly transcribed region in genome. Transposons are useful devices active parts genomes, and PiggyBac (PB) transposon can be considered as good option. In present study, three PB donor containing both heavy light chains were constructed, one contained...
In recent years, monoclonal antibodies (mAbs) have been developed as powerful therapeutic and diagnostic agents Chinese hamster ovary (CHO) cells emerged the dominant host for recombinant expression of these proteins. A critical step in is utilization strong promoters, such Hamster Elongation Factor-1α (CHEF-1) promoter. To compare strengths CHEF with cytomegalovirus (CMV) promoter mAb CHO cells, four bicistronic vectors bearing either internal ribosome entry site (IRES) or Furin/2A (F2A)...
Traditional methods to generate CHO cell lines rely on random integration(s) of the gene interest and result in unpredictable unstable protein expression. In comparison, site-specific recombination increase recombinant expression by inserting transgene at a locus with specific features. PhiC31 serine integrase, catalyze unidirectional integration that occurs higher frequency comparison reversible carried out recombinases such as Cre. this study, using different ratios phiC31 we evaluated...
Tuberculous granulomas are the sites of interaction between T cells, macrophages, and extracellular matrix (ECM) to control infection caused by Mycobacterium tuberculosis (M. tuberculosis). A predominant role RD-1-encoded secretory proteins, early secreted antigenic target-6 (ESAT-6), culture filtrate protein-10 (CFP-10) in formation has recently been emphasized. However, precise molecular events that induce these granulomatous structures yet be elucidated. Macrophages use integrins adhere...