A5087578365- Herpesvirus Infections and Treatments
- Virus-based gene therapy research
- Genomics and Chromatin Dynamics
- Toxin Mechanisms and Immunotoxins
- Cytomegalovirus and herpesvirus research
- Plant Molecular Biology Research
- Plant Gene Expression Analysis
- Plant Virus Research Studies
- Poxvirus research and outbreaks
- Polyomavirus and related diseases
- RNA and protein synthesis mechanisms
- Animal Virus Infections Studies
- Plant Stress Responses and Tolerance
- DNA Repair Mechanisms
- Ubiquitin and proteasome pathways
- RNA Interference and Gene Delivery
- T-cell and Retrovirus Studies
- RNA modifications and cancer
- DNA and Nucleic Acid Chemistry
- Viral Infections and Immunology Research
- RNA regulation and disease
- RNA Research and Splicing
- HIV Research and Treatment
- Glycosylation and Glycoproteins Research
- Bacteriophages and microbial interactions
Van Andel Institute
2008-2017
Michigan State University
2001-2011
University of Wisconsin–Madison
1998
Taos Orthopaedic Institute
1998
University of California, Los Angeles
1998
Howard Hughes Medical Institute
1998
Rutgers, The State University of New Jersey
1998
Keystone Symposia On Molecular and Cellular Biology
1998
National Institute of Allergy and Infectious Diseases
1995
University of Washington
1994
Mature virions of herpes simplex virus type 1 contain an activating factor that primes transcription from the five virally encoded immediate early (IE) genes. This activator is specified by a 65-kD polypeptide termed VP16. The action VP16 mediated through cis-regulatory elements located in regions adjacent to each IE gene. Although normally introduced into cells infecting virions, its trans-activating function can also be observed cotransfecting with plasmid encodes along reporter gene...
Virion protein 16 (VP16) of herpes simplex virus type 1 contains an acidic transcriptional activation domain. Missense mutations within this domain have provided insights into the structural elements critical for its function. Net negative charge contributed to, but was not sufficient for, by VP16. A putative amphipathic alpha helix did appear to be important component phenylalanine residue at position 442 exquisitely sensitive mutation. Transcriptional activators several classes contain...
Acidic transcriptional activation domains function well in both yeast and mammalian cells, some have been shown to bind the general transcription factors TFIID TFIIB. We now show that two acidic transactivators, herpes simplex virus VP16 human p53, directly interact with multisubunit factor TFIIH its Saccharomyces cerevisiae counterpart, b. The VP16- p53-binding these lie p62 subunit of homologous subunit, TFB1, Point mutations reduce transactivation activity cells weaken binding TFIIH. This...
Abstract We previously identified Arabidopsis genes homologous with the yeast ADA2 and GCN5 that encode components of ADA SAGA histone acetyltransferase complexes. In this report, we explore biological roles ADA2b genes. T-DNA insertion mutations in were found to have pleiotropic effects on plant growth development, including dwarf size, aberrant root short petals stamens flowers. Approximately 5% 8200 assayed by DNA microarray analysis showed changes expression mutants, three-fourths which...
The viral genes first expressed upon lytic infection by herpes simplex virus type 1 (HSV-1) encode the five immediate early (IE) proteins. IE gene expression is potently and specifically induced a virion protein termed VP16. Previous studies have shown that activating properties of VP16 are specific mediated upstream regulatory elements common to each gene. Paradoxically, however, does not appear be sequence-specific DNA-binding protein. To understand specificity activation, we identified...
Structural features of the transcriptional activation domain herpes simplex virion protein VP16 were examined by oligonucleotide-directed mutagenesis. Extensive mutagenesis at position 442 truncated (delta 456), normally occupied a phenylalanine residue, demonstrated importance an aromatic amino acid that position. On basis alignment sequence surrounding Phe-442 and sequences other domains, we subjected leucine residues positions 439 444 to Results from these experiments suggest bulky...
During infection by herpes simplex virus type 1 (HSV-1), the virion protein VP16 activates transcription of viral immediate-early (IE) genes. Genetic and biochemical assays have shown that potent transcriptional activation domain can associate with general factors chromatin-modifying coactivator proteins several types. The latter interactions are particularly intriguing because previous reports indicate HSV-1 DNA does not become nucleosomal during lytic infection. In present work, chemical...
Lesions resulting from recurrent genital herpes simplex virus (HSV) infection are characterized by infiltration of CD4+ lymphocytes. We have investigated the antigenic specificity 47 HSV-specific T-cell clones recovered HSV-2 buttock and thigh lesions five patients. Clones with proliferative responses to recombinant truncated glycoprotein B (gB) or gD purified natural gC comprised a minority total number isolated lesions. The gC2- gD2-specific had cytotoxic activity. approximate locations...
Acidic transcriptional activation domains function well in both yeast and mammalian cells, some have been shown to bind the general transcription factors TFIID TFIIB. We now show that two acidic transactivators, herpes simplex virus VP16 human p53, directly interact with multisubunit factor TFIIH its Saccharomyces cerevisiae counterpart, b. The VP16- p53-binding these lie p62 subunit of homologous subunit, TFB1, Point mutations reduce transactivation activity cells weaken binding TFIIH. This...
The transcriptional activation domain of the herpesvirus protein VP16 resides in carboxyl-terminal 78 amino acids (residues 413-490). Fluorescence analyses this indicated that critical are solvent-exposed highly mobile segments. To examine interactions between and components basal machinery, we incorporated (at position 442 or 473 VP16) tryptophan analogs can be selectively excited complexes with other Trp-containing proteins. TATA-box binding (TBP) (but not transcription factor B (TFIIB))...
During lytic infection by herpes simplex virus type 1 (HSV-1), histones are present at relatively low levels on the viral genome. However, mechanisms that account for such levels--how histone deposition genome is blocked or how removed from genome--are not yet defined. In this study, we show occupancy gradually increased with time when transcription of immediate-early (IE) genes was inhibited either deletion VP16 activation domain chemical inhibition RNA polymerase II (RNAP II). Inhibition...
The polyomavirus enhancer is required in cis for high-level expression of the viral early region and replication genome. We introduced multiple mutations which reduced transcription DNA replication. Polyomaviruses with these mutant enhancers formed very small plaques whole mouse embryo cells. Revertants mutants were isolated characterized. Reversion occurred by any following events: restoration guanosines at nucleotide (nt) 5134 nt 5140 within adenovirus 5 E1A core AGGAAGTGACT; acquisition...
ABSTRACT Virion protein 16 (VP16) of herpes simplex virus type 1 (HSV-1) is a potent transcriptional activator viral immediate-early (IE) genes. The VP16 activation domain can recruit various coactivators to target gene promoters. However, the role in HSV-1 IE expression during lytic infection had not been fully defined. We showed previously that such as p300 and CBP histone acetyltransferases BRM Brg-1 chromatin remodeling complexes are recruited promoters manner dependent mostly on...