- Insect behavior and control techniques
- Insect symbiosis and bacterial influences
- Insect-Plant Interactions and Control
- CRISPR and Genetic Engineering
- Insect Resistance and Genetics
- Insect Utilization and Effects
- Mosquito-borne diseases and control
- Insect and Pesticide Research
- Insect Pest Control Strategies
- Cultural Heritage Materials Analysis
- Building materials and conservation
- Microbial Natural Products and Biosynthesis
- Forest Insect Ecology and Management
- Genomics and Phylogenetic Studies
- Fungal Biology and Applications
- Neurobiology and Insect Physiology Research
- Entomopathogenic Microorganisms in Pest Control
- Conservation Techniques and Studies
- Insect and Arachnid Ecology and Behavior
- Forest Ecology and Biodiversity Studies
FAO/IAEA Agriculture and Biotechnology Laboratories
2025
International Atomic Energy Agency
2017-2023
BOKU University
2017-2022
University of Thessaly
2012-2018
Abstract Mass releases of sterilized male insects, in the frame sterile insect technique programs, have helped suppress pest populations since 1950s. In major horticultural pests Bactrocera dorsalis, Ceratitis capitata , and Zeugodacus cucurbitae a key phenotype white pupae (wp) has been used for decades to selectively remove females before releases, yet gene responsible remained unknown. Here, we use classical modern genetic approaches identify functionally characterize causal wp −...
The spotted wing
Abstract Paper documents in archives, libraries, and museums often undergo biodeterioration by microorganisms. Fungi less bacteria have been described to advance paper staining, so called “foxing” degradation of substrates. In this study, for the first time, fungal bacterial diversity biodeteriorated Hellenic General State Archives dating back 19th 20th century has assessed culture‐dependent independent methods. The internally transcribed spacer ( ITS ) region 16S rRNA gene were amplified...
Abstract Background Aedes aegypti is expected to invade previously unoccupied areas, mainly due the climate change, increase in travel and trade activities continuous transformation of rural environment into urban areas. The sterile insect technique (SIT), which relies on mass production release males, an environmentally friendly approach that can be applied for population control Ae. aegypti. SIT programs greatly benefited by a genetic sexing strain (GSS) reliable sex sorting system...
The Asian tiger mosquito, Aedes albopictus, is an invasive species and a vector for several significant human pathogens. Gaining deeper understanding of its reproductive biology offers valuable insights into evolutionary success may inform the development sustainable strategies to control spread. This study presents comprehensive structural functional characterization fruitless gene in Ae. albopictus (Aalfru), pivotal regulator sexual behavior insects. Through silico analysis combined with...
Abstract Since its recent invasion of the European and American continents, spotted wing Drosophila suzukii has become a burden fruit industry. Armed with highly sclerotized ovipositor, females can lay eggs in wider variety ripening healthy fruits than other species. Economic losses due to D. reach millions dollars annually methods control natural populations field mainly rely on use chemical pesticides. We tested if Wolbachia bacteria represents potential ally this pest. These symbionts are...
Abstract Background The Mediterranean fruit fly, Ceratitis capitata, is a cosmopolitan agricultural pest of worldwide economic importance and model for the development Sterile Insect Technique (SIT) flies Tephritidae family (Diptera). SIT relies on effective mating laboratory-reared strains natural populations, therefore requires an efficient mass-rearing system that will allow production high-quality males. Adaptation wild to artificial laboratory environment can be accompanied by negative...
Abstract Aedes aegypti is a major vector of arboviruses that cause dengue, chikungunya, yellow fever, and Zika. Although recent success in reverse genetics has facilitated rapid progress basic applied research, integration forward with modern technologies remains challenging this important species, as up to 47% its chromosome refractory genetic mapping due extremely low rate recombination. Here, we report the development marker-assisted strategy readily screen for genotype only rare but...
Drosophila suzukii (Diptera: Drosophilidae) is an invasive pest of a wide range commercial soft-skinned fruits. To date, most management tactics are based on spraying conventional and/or organic insecticides, baited traps, and netting exclusion. Interest has been expressed in using the sterile insect technique (SIT) as part area-wide integrated (AW-IPM) programs to control D. infestations. Mass-rearing protocols one prerequisites for successful implementation SIT. establish mass-rearing...
Treating insects with a lower oxygen atmosphere before and during exposure to radiation can mitigate some of the negative physiological effects due irradiation. The irradiation pupae under oxygen-reduced environment such as hypoxia or anoxia is routinely used in sterile insect technique (SIT) tephritid species it provides radiological protection. This treatment allows have already sealed containers facilitating shipment. SIT an friendly control tactic that could be manage populations...
Area-wide integrated pest management (AW-IPM) programmes with a sterile insect technique component (SIT) are used to control populations of pests worldwide, including the Mediterranean fruit fly, Ceratitis capitata. SIT consists mass rearing, radiation-induced sterilization, handling, and release insects over target area. Although can be performed by using both males females, male-only releases significantly increase efficiency cost-effectiveness applications. Male-only achieved genetic...
Abstract Background The Mediterranean fruit fly, Ceratitis capitata , is a significant agricultural pest managed through area-wide integrated management (AW-IPM) including sterile insect technique (SIT) component. Male-only releases increase the efficiency and cost-effectiveness of SIT programs, which can be achieved development genetic sexing strains (GSS). most successful GSS developed to date C. VIENNA 8 GSS, constructed using classical approaches an irradiation-induced translocation with...
The Spotted-Wing Drosophila fly, suzukii, is an invasive pest species infesting major agricultural soft fruits. suzukii management currently based on insecticide applications that bear concerns regarding their efficiency, safety and environmental sustainability. sterile insect technique (SIT) efficient friendly to the environment control method has been suggested for D. population control. Successful SIT require mass-rearing of strain produce competitive high biological quality males will be...
Abstract Genetic sexing strains (GSS) are an important tool in support of sterile insect technique (SIT) applications against pests and disease vectors. The yet unknown temperature-sensitive lethal (tsl) gene the recently identified white pupae (wp) have been used as selectable markers most successful GSS developed so far, Ceratitis capitata (medfly) VIENNA 8 GSS. molecular identification tsl may open way for its use a marker development other vectors SIT importance. Prior studies already...
is an invasive mosquito species and major vector of human arboviruses. A wide variety control methods have been employed to combat populations. One them the sterile insect technique (SIT) that has recently attracted considerable research efforts due its proven record success absence harmful environmental footprints. The efficiency cost-effectiveness SIT significantly enhanced by male-only releases. For SIT, releases are ideally needed since females bite, blood-feed transmit pathogens.
Abstract Mass releases of sterilized male insects, in the frame sterile insect technique programs, have helped suppress pest populations since 1950s. In major horticultural pests Bactrocera dorsalis, Ceratitis capitata , and Zeugodacus cucurbitae a key phenotype white pupae (wp) has been used for decades to selectively remove females before releases, yet gene responsible remained unknown. Here we use classical modern genetic approaches identify functionally characterize causal wp − mutations...
Abstract Aedes aegypti is a major vector of arboviruses that cause dengue, chikungunya, yellow fever and Zika. Although recent success in reverse genetics has facilitated rapid progress basic applied research, integration forward with modern technologies remains challenging this important species, as up-to-47% its chromosome refractory to genetic mapping due extremely low rate recombination. Here we report the development marker-assisted-mapping (MAM) strategy readily screen for genotype...