A5036427066- Plant Molecular Biology Research
- Chromosomal and Genetic Variations
- CRISPR and Genetic Engineering
- Insect behavior and control techniques
- Photosynthetic Processes and Mechanisms
- Genetic Mapping and Diversity in Plants and Animals
- Genomics and Phylogenetic Studies
- Plant nutrient uptake and metabolism
- Insect symbiosis and bacterial influences
- Plant Stress Responses and Tolerance
- Plant Genetic and Mutation Studies
- Plant tissue culture and regeneration
- Plant Disease Resistance and Genetics
- Light effects on plants
- Legume Nitrogen Fixing Symbiosis
- Genomics and Chromatin Dynamics
- Plant Virus Research Studies
- Machine Learning in Bioinformatics
- Insect Resistance and Genetics
- RNA Research and Splicing
- Insect-Plant Interactions and Control
- Plant Gene Expression Analysis
- Genetic and phenotypic traits in livestock
- Horticultural and Viticultural Research
- Plant Reproductive Biology
Agriculture and Food
2020-2025
South Australian Research and Development Institute
2017-2025
The University of Adelaide
2019-2025
The University of Queensland
2020-2025
Australian Research Council
2009-2022
Australian National University
2009-2022
University of Minnesota
2017-2022
Macquarie University
2021
Brisbane School of Theology
2020
University of California, Davis
2019
Compartmentation of the eukaryotic cell requires a complex set subcellular messages, including multiple retrograde signals from chloroplast and mitochondria to nucleus, regulate gene expression. Here, we propose that one such signal is phosphonucleotide (3'-phosphoadenosine 5'-phosphate [PAP]), which accumulates in Arabidopsis thaliana response drought high light (HL) stress enzyme SAL1 regulates its levels by dephosphorylating PAP AMP. chloroplasts but not cytosol. sal1 mutants accumulate...
Abstract Carotenoid pigments are critical for plant survival, and carotenoid composition is tuned to the developmental stage, tissue, environmental stimuli. We report cloning of CAROTENOID CHLOROPLAST REGULATORY1 (CCR1) gene. The ccr1 mutant has increased shoot branching altered composition, namely, reduced lutein in leaves accumulation cis-carotenes dark-grown seedlings. CCR1 gene was previously isolated as EARLY FLOWERING IN SHORT DAYS encodes a histone methyltransferase (SET DOMAIN GROUP...
Improving the responsiveness, acclimation, and memory of plants to abiotic stress holds substantive potential for improving agriculture. An unresolved question is involvement chromatin marks in agriculturally relevant stresses. Such has spurred numerous investigations yielding both promising conflicting results. Consequently, it remains unclear what extent robust stress-induced DNA methylation variation can underpin memory. Using a slow-onset water deprivation treatment Arabidopsis...
Organelle-nuclear retrograde signaling regulates gene expression, but its roles in specialized cells and integration with hormonal remain enigmatic. Here we show that the SAL1-PAP (3′-phosphoadenosine 5′- phosphate) pathway interacts abscisic acid (ABA) to regulate stomatal closure seed germination Arabidopsis. Genetically or exogenously manipulating PAP bypasses canonical components ABA Insensitive 1 (ABI1) Open Stomata (OST1); priming an alternative restores ABA-responsive ROS bursts, ion...
Sunlight provides energy for photosynthesis and is essential nearly all life on earth. However, too much or little light rapidly fluctuating conditions cause stress to plants. Rapid changes in the amount of are perceived as a change reduced/oxidized (redox) state photosynthetic electron transport components chloroplasts. how this generates signal that relayed nuclear gene expression not well understood. We modified redox reference plant, Arabidopsis thaliana, using either excess low plus...
Stress recovery may prove to be a promising approach increase plant performance and, theoretically, mRNA instability facilitate faster recovery. Transcriptome (RNA-seq, qPCR, sRNA-seq, and PARE) methylome profiling during repeated excess-light stress was performed at intervals as short 3 min. We demonstrate that 87% of the stress-upregulated mRNAs analyzed exhibit very rapid For instance, HSP101 abundance declined 2-fold every 5.1 term this phenomenon gene downregulation (RRGD), whereby...
DNA methylation can provide a source of heritable information that is sometimes entirely uncoupled from genetic variation. However, the extent this uncoupling and roles in shaping diversity both gene expression phenotypes are hotly debated. Here, we investigate basis biological functions at population scale maize.We perform targeted profiling for diverse panel 263 maize inbred genotypes. All genotypes show similar levels globally, highlighting importance development. Nevertheless, identify...
Significance Crop genomes can be very large, with many repetitive elements and pseudogenes. Distilling a genome down to the relatively small fraction of regions that are functionally valuable for trait variation like looking needles in haystack. The location these is often not obvious, current detection technologies impractically expensive intensive research projects. unmethylated highly stable during vegetative development reveal locations potentially expressed genes or cis-regulatory...
Abstract CRISPR–Cas9-mediated genome editing has been widely adopted for basic and applied biological research in eukaryotic systems. While many studies consider DNA sequences of CRISPR target sites as the primary determinant mutagenesis efficiency mutation profiles, increasing evidence reveals substantial role chromatin context. Nonetheless, most prior are limited by lack sufficient epigenetic resources and/or only transiently expressing CRISPR–Cas9 a short time window. In this study, we...
Abstract DNA methylation can contribute to the maintenance of genome integrity and regulation gene expression. In most situations, patterns are inherited quite stably. However, changes in occur at some loci as a result tissue culture resulting somaclonal variation. To investigate heritable epigenetic consequence culture, sequence-capture bisulfite sequencing approach was implemented monitor context-specific ∼15 Mb maize for population plants that had been regenerated from culture. Plants...
DNA methylation and epigenetic silencing play important roles in the regulation of transposable elements (TEs) many eukaryotic genomes. A majority maize genome is derived from TEs that can be classified into different orders families based on their mechanism transposition sequence similarity, respectively. themselves are highly methylated it tempting to view them as a single uniform group. However, analysis profiles flanking regions provides evidence for distinct groups chromatin properties...
Abstract The regulation of gene expression is central to many biological processes. Gene regulatory networks (GRNs) link transcription factors (TFs) their target genes and represent maps potential transcriptional regulation. Here, we analyzed a large number publically available maize (Zea mays) transcriptome data sets including >6000 RNA sequencing samples generate 45 coexpression-based GRNs that relationships between TFs other in different populations (cross-tissue, cross-genotype,...
SUMMARY In recent years, Setaria viridis has been developed as a model plant to better understand the C4 photosynthetic pathway in major crops. With increasing availability of genomic resources for S. research, highly efficient genome editing technologies are needed create genetic variation functional genomics. Here, we protoplast assay rapidly optimize multiplexed clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR‐associated (Cas9) system . Targeted mutagenesis...
Abstract Mass releases of sterilized male insects, in the frame sterile insect technique programs, have helped suppress pest populations since 1950s. In major horticultural pests Bactrocera dorsalis, Ceratitis capitata , and Zeugodacus cucurbitae a key phenotype white pupae (wp) has been used for decades to selectively remove females before releases, yet gene responsible remained unknown. Here, we use classical modern genetic approaches identify functionally characterize causal wp −...
Changes in gene expression are important for responses to abiotic stress. Transcriptome profiling of heat- or cold-stressed maize genotypes identifies many changes transcript abundance. We used comparisons multiple identify alleles with variable heat cold stress and distinguish examples cis- trans-regulatory variation stress-responsive changes. motifs enriched near the transcription start sites (TSSs) thermal genes develop predictive models responses. Prediction accuracies can be improved by...
Distinct ROS signaling pathways initiated by singlet oxygen ((1)O2) or superoxide and hydrogen peroxide have been attributed to either cell death acclimation, respectively. Recent studies revealed that more complex antagonistic synergistic relationships exist within between these pathways. As specific chloroplastic signals are difficult study, rapid systemic experiments using localized high light (HL) stress treatments were used in this study uncouple required for direct HL perception distal...
Abstract The Queensland fruit fly, Bactrocera tryoni (Froggatt), is a polyphagous horticultural pest in Australia that capable of causing significant damage to more than 100 different host fruits and vegetables. Chemical applications ecological control strategies, such as the sterile insect technique ( SIT ), are commonly used suppress established populations eradicate invasive outbreaks following migration. recently published B. draft genome provides new opportunities identify candidate...
Background Mutations in the FRY1/SAL1 Arabidopsis locus are highly pleiotropic, affecting drought tolerance, leaf shape and root growth. FRY1 encodes a nucleotide phosphatase that vitro has inositol polyphosphate 1-phosphatase 3′,(2′),5′-bisphosphate activities. It is not clear which activity mediates each of diverse biological functions planta. Principal Findings A fry1 mutant was identified genetic screen for mutants deregulated expression Pi High affinity Transporter 1;4 (PHT1;4)....
Abstract The capacity for plant stress priming and memory the notion of this being underpinned by DNA methylation‐mediated is an appealing hypothesis which there mixed evidence. We previously established a lack drought‐induced methylome variation in Arabidopsis thaliana (Arabidopsis); however, was tied to only minor observations physiological memory. There are numerous independent demonstrating that photoprotective mechanisms, induced excess‐light stress, can lead robust programmable changes...
To further our understanding of how sustained changes in temperature affect the carbon economy rice (Oryza sativa), hydroponically grown plants IR64 cultivar were developed at 30°C/25°C (day/night) before being shifted to 25/20°C or 40/35°C. Leaf messenger RNA and protein abundance, sugar starch concentrations, gas-exchange elongation rates measured on preexisting leaves (PE) already 30/25°C newly (ND) subsequent transfer. Following a shift growth temperature, there was transient adjustment...
Plants are subjected to extreme environmental conditions and must adapt rapidly. The phytohormone abscisic acid (ABA) accumulates during abiotic stress, signaling transcriptional changes that trigger physiological responses. Epigenetic modifications often facilitate transcription, particularly at genes exhibiting temporal, tissue-specific environmentally-induced expression. In maize (Zea mays), MEDIATOR OF PARAMUTATION 1 (MOP1) is required for progression of an RNA-dependent epigenetic...