A5089069549- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- CRISPR and Genetic Engineering
- IL-33, ST2, and ILC Pathways
- Extracellular vesicles in disease
- Hematopoietic Stem Cell Transplantation
- Immune Response and Inflammation
- Single-cell and spatial transcriptomics
- Gene Regulatory Network Analysis
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Immune cells in cancer
- Mesenchymal stem cell research
- Cell Image Analysis Techniques
- RNA and protein synthesis mechanisms
- Reproductive System and Pregnancy
- Acute Myeloid Leukemia Research
- Cytokine Signaling Pathways and Interactions
National Institute of Dental and Craniofacial Research
2019-2022
National Institutes of Health
2019-2020
National Cancer Institute
2004-2009
Science Applications International Corporation (United States)
2004-2009
Cancer Research Center
2009
Highlights•A CRISPR interference platform for genetic screens in human iPSC-derived neurons•Survival uncover genes essential neurons, but not iPSCs or cancer cells•Single-cell RNA-seq reveal distinct neuronal roles ubiquitous genes•Arrayed high-content controlling morphologySummaryCRISPR/Cas9-based functional genomics have transformed our ability to elucidate mammalian cell biology. However, most previous CRISPR-based were conducted lines rather than healthy, differentiated cells. Here, we...
Interleukin-7 (IL-7) is required for lymphocyte development and homeostasis although the actual sites of IL-7 production have never been clearly identified. We produced a bacterial artificial chromosome (BAC) transgenic mouse expressing ECFP in Il7 locus. The construct lacked signal peptide (enhanced cyan fluorescent protein ) accumulated inside IL-7-producing stromal cells thoracic thymus, cervical thymus bone marrow. In an extensive reticular network IL-7-containing processes extended from...
It is commonly believed that IL-12 produced by DCs in response to pathogens the first signal stimulates production of IFN-γ NK cells. However, bacterial LPS depends on either engagement CD40 CD40L activated T cells or from This suggests during primary immune response, produce before DCs. Here, using single-cell measurements, cell sorting and mouse lines deficient IL-12, IL-23, type I IFN receptor IL-18 receptor, we show a subset BM-derived characterized low expression MHC class II (MHCII
Abstract Hemopoiesis depends on the expression and regulation of transcription factors, which control maturation specific cell lineages. We found that helix-loop-helix factor inhibitor DNA-binding protein 1 (Id1) is not expressed in hemopoietic stem cells (HSC), but increased more committed myeloid progenitors. Id1 levels decrease during neutrophil differentiation, remain high differentiated macrophages. at low or absent developing lymphoid erythroid cells. can be induced by IL-3 HSC growth...
Sarcoidosis is a devastating inflammatory disease affecting many organs, especially the lungs and lymph nodes. Bone marrow-derived mesenchymal stromal cells (MSCs) can "reprogram" various types of macrophages towards an anti-inflammatory phenotype. We wanted to determine whether alveolar from sarcoidosis subjects behave similarly by mounting response when co-cultured with MSCs. Fifteen eight control underwent bronchoscopy bronchoalveolar lavage (BAL). Unselected BAL (70-94% macrophages) were...
Abstract Dendritic cells (DCs) play a key role in the initiation of an immune response and are known as “professional” APCs because their ability to activate naive T cells. A widely used method generate DCs vitro is culture bone marrow (BM) or blood monocytes presence GM-CSF IL-4. In this study, we show that small population NK residing BM RAG−/−, but not RAG−/− γc chain−/− mice, remain DC source IFN-γ produced after stimulation with LPS. These cells, which may represent early promoters...
SUMMARY CRISPR/Cas9-based functional genomics have transformed our ability to elucidate mammalian cell biology. However, most previous CRISPR-based screens were conducted in cancer lines, rather than healthy, differentiated cells. Here, we describe a CRISPR interference (CRISPRi)-based platform for genetic human neurons derived from induced pluripotent stem cells (iPSCs). We demonstrate robust and durable knockdown of endogenous genes such neurons, present results three complementary...