A5081855463- Sepsis Diagnosis and Treatment
- Bacterial Identification and Susceptibility Testing
- Escherichia coli research studies
- Bacterial Genetics and Biotechnology
- Antibiotic Resistance in Bacteria
- Neonatal and Maternal Infections
- Vibrio bacteria research studies
- Antimicrobial Resistance in Staphylococcus
- Clinical Laboratory Practices and Quality Control
- Streptococcal Infections and Treatments
- Genomics and Phylogenetic Studies
- Hemodynamic Monitoring and Therapy
- Gut microbiota and health
- Salmonella and Campylobacter epidemiology
- Clostridium difficile and Clostridium perfringens research
- T-cell and B-cell Immunology
- RNA and protein synthesis mechanisms
- Inflammatory Biomarkers in Disease Prognosis
- Electronic Health Records Systems
- Biomedical Text Mining and Ontologies
- Clusterin in disease pathology
- Plant Pathogenic Bacteria Studies
- Genetic factors in colorectal cancer
- RNA Interference and Gene Delivery
- Bacterial biofilms and quorum sensing
University of Skövde
2005-2023
Karolinska Institutet
2001-2006
Background Early recognition is a key factor to achieve improved outcomes for septic patients. Combinations of biomarkers, as opposed single ones, may improve timely diagnosis and survival. We investigated the performance characteristics sepsis alone in combination, verified bacterial using Sepsis-2 Sepsis-3 criteria, respectively. Methods Procalcitonin (PCT), neutrophil-lymphocyte count ratio (NLCR), C-reactive protein (CRP), lactate were determined total 1,572 episodes adult patients...
ABSTRACT The global regulator CsrA (carbon storage regulator) is an RNA binding protein that coordinates central carbon metabolism, activates flagellum biosynthesis and motility, represses biofilm formation in Escherichia coli . activity antagonized by the untranslated CsrB, to which it binds forms a globular ribonucleoprotein complex. indirectly csrB transcription, apparent autoregulatory mechanism. In present study, we elucidate intermediate regulatory circuitry of this system. Mutations...
BarA is a membrane-associated protein that belongs to subclass of tripartite sensors the two-component signal transduction system family. In this study, we report UvrY cognate response regulator for Escherichia coli. This conclusion based upon homologies with analogous systems and demonstrated by both biochemical genetic means. We show purified able autophosphorylate when incubated [gamma-(32)P]ATP but not [alpha-(32)P]ATP or [gamma-(32)P]GTP. Phosphorylated BarA, in turn, acts as an...
ABSTRACT The Escherichia coli BarA and UvrY proteins were recently demonstrated to constitute a novel two-component system, although its function has remained largely elusive. Here we show that mutations in the sensor kinase gene, barA , or response regulator uvrY uropathogenic E. drastically affect survival long-term competition cultures. Using media with gluconeogenic carbon sources, mutants have clear growth advantage when competing wild type, but using sources feeding into glycolysis...
Sepsis is a serious medical condition requiring timely administered, appropriate antibiotic therapy. Blood culture regarded as the gold standard for aetiological diagnosis of sepsis, but it suffers from low sensitivity and long turnaround time. Thus, nucleic acid amplification tests (NAATs) have emerged to shorten time identification causative microbes. The aim present study was evaluate clinical utility in everyday practice emergency department two commercial NAATs patients suspected with...
Abstract Background The Salmonella enterica BarA-SirA, the Erwinia carotovora ExpS-ExpA, Vibrio cholerae BarA-VarA and Pseudomonas spp GacS-GacA all belong to same orthologous family of two-component systems as Escherichia coli BarA-UvrY. In first four species it has been demonstrated that disruption this system leads a clear reduction in virulence bacteria. Our aim was determine if BarA-UvrY is connected with using monkey cystitis model. Results Cystitis generated Macaque fascularis monkeys...
Klebsiella is a genus of Gram-negative bacteria known to be opportunistic pathogens that may cause variety infections in humans. Highly drug-resistant species, especially K. pneumoniae , have emerged rapidly and are becoming major concern clinical management. Although considered the most important pathogen within genus, true significance other species likely underrecognized due inability conventional microbiological methods distinguish between leading high rates misidentification. Bacterial...
ABSTRACT The BarA-UvrY two-component system family is strongly associated with virulence but poorly understood at the molecular level. During our attempts to complement a barA deletion mutant, we consistently generated various mutated BarA proteins. We reasoned that characterization of mutants would help us better understand signal transduction mechanism in tripartite sensors. This was aided by demonstrated ability activate UvrY regulator acetyl phosphate independently sensor. Many proteins...
Background: Early detection of bacteria and their antibiotic susceptibility patterns are critical to guide therapeutic decision-making for optimal care septic patients. The current gold standard, blood culturing followed by subculture on agar plates subsequent identification, is too slow leading excessive use broad-spectrum with harmful consequences the patient and, in long run, public health. aim present study was assess performance two commercial assays, QuickFISH® (OpGen) Maldi...
Neutrophil to lymphocyte count ratio performs better than procalcitonin as a biomarker for bacteremia and severe sepsis in the emergency department
Extra-intestinal pathogenic Escherichia coli (ExPEC) strains are responsible for a large number of human infections globally. The management caused by ExPEC has been complicated the emergence antimicrobial resistance, most importantly increasing recognition isolates producing extended-spectrum β-lactamases (ESBL). Herein, we used whole-genome sequencing (WGS) on comprehensive genotypic characterization. Twenty-one isolates, nine with and 12 without ESBL-production, from 16 patients suspected...
Abstract Background The rapidly growing area of sequencing technologies, and more specifically bacterial whole-genome sequencing, could offer applications in clinical microbiology, including species identification bacteria, prediction genetic antibiotic susceptibility virulence genes simultaneously. To accomplish the aforementioned points, commercial cloud-based platform, 1928 platform (1928 Diagnostics, Gothenburg, Sweden) was benchmarked against an in-house developed bioinformatic pipeline...