A5032936559- Pain Mechanisms and Treatments
- Viral Infectious Diseases and Gene Expression in Insects
- Fibromyalgia and Chronic Fatigue Syndrome Research
- Animal Genetics and Reproduction
- CRISPR and Genetic Engineering
- Exercise and Physiological Responses
- Neuropeptides and Animal Physiology
- Phagocytosis and Immune Regulation
- Transgenic Plants and Applications
- Neuroinflammation and Neurodegeneration Mechanisms
- Pain Management and Placebo Effect
- Monoclonal and Polyclonal Antibodies Research
- Pain Management and Opioid Use
- Immunotherapy and Immune Responses
- Contact Dermatitis and Allergies
- Erythrocyte Function and Pathophysiology
- Nerve injury and regeneration
- Antifungal resistance and susceptibility
- Livestock and Poultry Management
- T-cell and B-cell Immunology
- Botulinum Toxin and Related Neurological Disorders
- Food Allergy and Anaphylaxis Research
- Allergic Rhinitis and Sensitization
- Histone Deacetylase Inhibitors Research
- Blood properties and coagulation
National Institute of Advanced Industrial Science and Technology
2020-2024
Biomedical Research Institute
2021-2024
Kobe Pharmaceutical University
2018-2024
Nagasaki University
2011-2016
Background and Purpose Hypoesthesia is a clinical feature of neuropathic pain. The partly explained by the evidence epigenetic repression Na v 1.8 sodium channel in dorsal root ganglion ( DRG ). Experimental Approach We investigated possibility trichostatin A TSA ), valproic acid VPA ) suberoylanilide hydroxamic SAHA to reverse unique C ‐fibre sensitivity observed following partial ligation sciatic nerve mice. Key Results Nerve injury‐induced down‐regulation ‐fibre‐related hypoesthesia were...
Abstract Neudesin, originally identified as a neurotrophic factor, has primarily been studied for its neural functions despite widespread expression. Using 8-week-old neudesin knockout mice, we elucidated the role of in spleen. The absence caused mild splenomegaly, shortened lifespan circulating erythrocytes, and abnormal recovery from phenylhydrazine-induced acute anemia. Blood cross-transfusion splenectomy experiments revealed that erythrocytes was attributable to splenic impairment....
Background: Fibromyalgia (FM) is characterized by chronic widespread pain, which often refractory to conventional painkillers. Numerous clinical studies have demonstrated that antidepressants are effective in treating FM pain. We previously established a mouse model of FM-like induced intermittent cold stress (ICS). Results: In this study, we find ICS exposure causes transient increase plasma corticosterone concentration, but not anxiety or depression-like behaviors. A single intrathecal...
Neuropathic pain is often insensitive to morphine. Our previous study has demonstrated that neuron-restrictive silencer factor represses mu opioid receptor (MOP) gene expression in the dorsal root ganglion (DRG) via histone hypoacetylation-mediated mechanisms after peripheral nerve injury, thereby causing loss of morphine analgesia. Here, we showed deacetylase (HDAC) inhibitors, such as trichostatin A and valproic acid, restored systemic analgesia neuropathic pain. Also, these agents blocked...
Increased commercial demand for monoclonal antibodies (mAbs) has resulted in the urgent need to establish efficient production systems. We previously developed a transgenic chicken bioreactor system that effectively produced human cytokines egg whites using genome-edited chickens. Here, we describe application of this mAb production. The genes encoding heavy and light chains humanized anti-HER2 mAb, linked by 2A peptide sequence, were integrated into ovalbumin gene locus CRISPR/Cas9...
Herein, we investigated the role of periaqueductal gray (PAG)-resident microglia in development morphine tolerance and its underlying mechanisms. We showed that clodronate minocycline known as inhibitors reversed tolerance, providing proof activation has key tolerance. The microglia-mediated anti-opioid mechanism occurs via sequential BDNF release NMDA expression. Experimental evidence is provided here conditional bdnf knockout mice (bdnf-/-) failed to develop following Cre-recombinase...
Ovomucoid is a major egg white protein which considered as the most dominant allergen in chicken eggs. Owing to difficulty of separating ovomucoid from whites, researchers have adopted genetic deletion for development hypoallergenic Previously, we used CRISPR/Cas9 establish chickens with gene (OVM) mutations, but it remained unknown whether such hens could produce eggs at maturity. Here, reported on laid by OVM-targeted hens. Except watery had no evident abnormalities. Real-time PCR revealed...
Treatment of fibromyalgia is an unmet medical need. To develop novel therapies for the treatment fibromyalgia, we explored pain therapeutic actions existing pharmaceuticals, which inhibit somatic symptoms frequently observed in patients. This study first examined pilocarpine, inhibits dry-eye and dry-mouth symptoms, using experimental fibromyalgia-like chronic model produced by intermittent cold stress (ICS) mice. A single intraperitoneal intracerebroventricular, but not intrathecal,...
Pregabalin (PGB) is a valuable therapeutic drug against chronic pain. Here we attempted to perform the combinatorial therapy with P-glycoprotein (P-gp) inhibitors lower dosage of PGB in intermittent cold stress-induced fibromyalgia-like pain model. Single intracerebroventricular (i.c.v.) injection exerted long-lasting anti-hyperalgesic effects for 72 h, while effect given intraperitoneally (i.p.) disappeared within 3 h. Importantly, pretreatment P-gp markedly prolonged effects, which lasted...
Transgenic chicken bioreactors can efficiently produce egg whites containing large quantities of recombinant proteins. We previously developed transgenic chickens that monoclonal antibodies (mAbs) against epidermal growth factor receptor 2 (HER2). However, the practical applications mAbs derived from eggs have not yet been examined. Therefore, we aimed to evaluate whether these be used in enzyme-linked immunosorbent assay (ELISA). Recombinant HER2 were dissolved phosphate-buffered saline and...
Increased commercial demand for monoclonal antibodies has resulted in an urgent need to establish alternative production systems. We previously developed a transgenic chicken bioreactor system that efficiently produced human cytokines egg whites from genome-edited chickens. Here, we report on the application of this antibody production. The genes encoding heavy and light chains humanized anti-HER2 mAb linked by 2A peptide sequence were integrated into ovalbumin locus using CRISPR/Cas9...