A5009186658- Lung Cancer Treatments and Mutations
- Cancer Genomics and Diagnostics
- Thyroid Cancer Diagnosis and Treatment
- Molecular Biology Techniques and Applications
- Cervical Cancer and HPV Research
- Pancreatic and Hepatic Oncology Research
- Lung Cancer Diagnosis and Treatment
- Lung Cancer Research Studies
- Cutaneous Melanoma Detection and Management
- Melanoma and MAPK Pathways
- Global Cancer Incidence and Screening
- Salivary Gland Tumors Diagnosis and Treatment
- CAR-T cell therapy research
- Cancer Research and Treatments
- Thyroid and Parathyroid Surgery
- Clinical Laboratory Practices and Quality Control
- BRCA gene mutations in cancer
- Neuroendocrine Tumor Research Advances
- Genetic factors in colorectal cancer
- Pancreatitis Pathology and Treatment
- Engineering Diagnostics and Reliability
- Head and Neck Surgical Oncology
- AI in cancer detection
- Breast Lesions and Carcinomas
- Gastric Cancer Management and Outcomes
Indiana University School of Medicine
2012-2023
Indiana University – Purdue University Indianapolis
2012-2023
Rush University Medical Center
2018
Columbia College - Missouri
2018
Indiana University Health
2014-2018
University Medical Center
2017
University Hospital and Clinics
2017
Indiana University
2017
HealthPartners
2015
Endoscopic ultrasound-guided fine needle aspiration (EUS-FNA) remains the most common EUS-guided tissue acquisition technique. This study aimed to evaluate performance of a new Franseen tip biopsy (FNB) device for EUSguided sampling solid lesions and compare it with historical FNA technique.Acquire® 22 G FNB (Boston Scientific Co., Natick, MA, USA) was used tumor (Study group). Tissue collected rapid on-site evaluation, touch crush preparations were made. Historical EUS-FNA samples obtained...
Intraductal papillary mucinous neoplasms (IPMN) have been considered difficult to diagnose by fine-needle aspiration (FNA) cytology. We identified 57 cases diagnosed as IPMN or consistent with endoscopic ultrasound (EUS)-guided FNA over a 9-yr period. Histologic follow-up was available for 20 patients (35%). Pancreatic neoplasia demonstrated in 18 of these (90%). The histologic diagnoses were (16 cases), pancreatic intraductal (grade 1b, 1 case), invasive mucin-producing adenocarcinoma (1...
The ability to produce adequate cell blocks profoundly impacts the diagnostic usefulness of cytology specimens. Cell are routinely processed from fine-needle aspiration specimens or concentrated fluid samples. Obtaining directed passes for sole purpose producing a block is common practice, particularly when cytopathologist anticipates need ancillary immunocytochemical stains and/or molecular studies.The authors developed an effective and inexpensive process that consistently yields abundant...
Abstract BACKGROUND The management of thyroid nodules with indeterminate cytology is challenging. Recently, molecular testing on fine‐needle aspirates (FNAs) has been advocated to determine whether clinical follow‐up or surgery warranted for patients. Three different platforms were performed from our institution (Afirma Thyroid FNA Analysis, RosettaGX Reveal, and Interpace ThyGenX/ThyraMIR). This study compares their diagnostic efficacy. METHODS We conducted a retrospective analysis FNAs...
Background: Anaplastic lymphoma kinase (ALK) immunohistochemical staining on formalin-fixed paraffin-embedded tissue or cell blocks (CB) has been reported as an effective alternative to fluorescence hybridization in situ (FISH) for the detection of ALK gene rearrangement. However, CB frequently lack adequate cellularity even when direct smears are cellular. This study aims assess utility immunocytochemical (ICC) using transfer (CT) technique Methods: Fine-needle aspiration (FNA) cases lung...
Background Ancillary molecular testing has been recommended for thyroid fine‐needle aspirates (FNA) with indeterminate cytologic diagnoses. Rosetta Genomics and Interpace Diagnostics have developed assays that can utilize direct smears as the substrate. Methods A retrospective study of FNAs known histologic follow‐up was performed. One Diff‐Quik‐stained smear one Papanicolaou‐stained similar cellularity (at least 60‐100 lesional cells) from each case were sent to Interpace, respectively,...
To determine the utility of cell transfer technique (CTT) for BRAF molecular testing on thyroid fine-needle aspiration (FNA) specimens. Polymerase chain reaction (PCR)–based was performed tissues obtained through CTT from both air-dried and ethanol-fixed direct smears FNA specimens then compared with corresponding thyroidectomy formalin-fixed, paraffin-embedded (FFPE) 30 cases. successfully 29 CTT, 27 FFPE tissues. The results exhibited 11, 13, 13 mutations 18, 14, 14 wild types tissues,...
Context.— In some instances the standard method of doing molecular testing from formalin-fixed, paraffin-embedded block is not possible because limited tissue. Tumor cell–enriched cell-transfer technique has been proven useful for performing immunocytochemistry and on cytologic smears. Objective.— To establish as a viable option isolating tumor cells hematoxylin-eosin (H&E)–stained slides. Design.— Molecular was performed by using 97 archived H&E-stained slides variety different...
Background Molecular tests are increasingly used to triage cytologically indeterminate thyroid nodules for surgery and/or follow‐up. We retrospectively compared the performance of Afirma Gene Expression Classifier (AGEC) with that more recently developed RosettaGX ® Reveal™ miRNA (Reveal) in a cohort Bethesda III–V FNAs surgical Design Eighty‐one samples (54 III, 26 IV, 1 V) available AGEC (74 AGEC‐SUSP and 7 AGEC‐BENIGN) pathology results were studied from three academic centers. Reveal was...
The aim of this study was to investigate epidermal growth factor receptor (EGFR) gene mutations and anaplastic lymphoma kinase (ALK) rearrangements using cytological specimens from the patients with a diagnosis primary or metastatic lung non-small cell carcinoma.A total 307 cases were submitted for EGFR mutational analysis 265 ALK analysis. specimen sources included lung, lymph node, liver, bone, adrenal gland, mesentery mass, body fluids/bronchial brushing. in exons 18 21 analyzed Qiagen...
BACKGROUND The frequency of unsatisfactory gynecologic specimens has increased in the study laboratory over last few years due to advent personal lubricants. Similarly, lysed blood, protein, and necrotic debris present a challenge terms negative cell transference caused by clogged filter. In current study, authors evaluated potential use modified SurePath reprocessing technique decrease specimens. METHODS An aliquot for human papillomavirus testing was set aside remaining specimen ThinPrep...
Background/Aims: Endoscopic ultrasound-guided fine needle aspiration (EUS-FNA) remains the most common EUS-guided tissue acquisition technique.This study aimed to evaluate performance of a new Franseen tip biopsy (FNB) device for EUSguided sampling solid lesions and compare it with historical FNA technique.Methods: Acquire ® 22 G FNB (Boston Scientific Co., Natick, MA, USA) was used tumor (Study group).Tissue collected rapid on-site evaluation, touch crush preparations were made.Historical...
<b><i>Objectives:</i></b> Fine-needle aspiration (FNA) is frequently used to diagnose metastatic melanoma. In this study, we validated the use of cell-transferred cytological smears for <i>BRAF</i> molecular testing. <b><i>Study Design:</i></b> We conducted a search our laboratory information system period 2011-2013 in order identify surgical pathology cases either primary or melanomas which mutation analyses had already been...
The human papillomavirus (HPV) status of squamous cell carcinomas (SCCs) the head and neck is relevant for therapy planning, staging, follow-up. Immunohistochemistry (IHC) p16 a surrogate marker HPV in oropharyngeal SCC, but not at other sites. We tested if cobas test was feasible superior to p16-IHC on fine-needle aspiration (FNA) supernatants frozen section (FS) scrapings suspected SCC. A 500 μL aliquots postcentrifugation supernatant CytoRich Red media FNA cellblock specimens FS suspended...