A5054562700- Developmental Biology and Gene Regulation
- Congenital heart defects research
- Pluripotent Stem Cells Research
- Genomics and Chromatin Dynamics
- Axon Guidance and Neuronal Signaling
- Hearing, Cochlea, Tinnitus, Genetics
- Spaceflight effects on biology
- Cellular transport and secretion
- Zebrafish Biomedical Research Applications
- Single-cell and spatial transcriptomics
- Neuroscience and Neural Engineering
- Connexins and lens biology
- Genetic Mapping and Diversity in Plants and Animals
- Neurogenesis and neuroplasticity mechanisms
- Gene expression and cancer classification
- Neurogenetic and Muscular Disorders Research
- Pancreatic function and diabetes
- Extracellular vesicles in disease
- Planarian Biology and Electrostimulation
- Underwater Vehicles and Communication Systems
- Ear Surgery and Otitis Media
- Neonatal Respiratory Health Research
- Magnetic and Electromagnetic Effects
- Ocular Disorders and Treatments
- Neuropeptides and Animal Physiology
IRCCS Humanitas Research Hospital
2025
Humanitas University
2020-2025
The Francis Crick Institute
2019-2020
Istituti di Ricovero e Cura a Carattere Scientifico
2020
King's College London
2011-2019
STMicroelectronics (United Kingdom)
2019
Neurosciences Paris-Seine
2018
Sorbonne Université
2018
Institut de Biologie Paris-Seine
2018
Inserm
2018
Abstract Background Although invasiveness is one of the major determinants poor glioblastoma (GBM) outcome, mechanisms GBM invasion are only partially understood. Among intrinsic and environmental processes promoting cell-to-cell interaction processes, eventually driving invasion, we focused on pro-invasive role played by Extracellular Vesicles (EVs), a heterogeneous group cell-released membranous structures containing various bioactive cargoes, which can be transferred from donor to...
ABSTRACT Segmentation of the vertebrate hindbrain leads to formation rhombomeres, each with a distinct anteroposterior identity. Specialised boundary cells form at segment borders that act as source or regulator neuronal differentiation. In zebrafish, there is spatial patterning neurogenesis in which non-neurogenic zones boundaries and centres, part mediated by Fgf20 signalling. To further understand control neurogenesis, we have carried out single cell RNA sequencing zebrafish three...
In vertebrates, cranial placodes contribute to all sense organs and sensory ganglia arise from a common pool of Six1/Eya2+ progenitors. Here we dissect the events that specify ectodermal cells as placode progenitors using newly identified genes upstream Six/Eya complex. We show in chick two different tissues, namely lateral head mesoderm prechordal mesendoderm, gradually induce progenitors: pass through successive transcriptional states, each by distinct factors controlled signals. Both...
Spinal muscular atrophy (SMA) is a severe neurological disease caused by mutations in the SMN1 gene, characterized early onset and degeneration of lower motor neurons. Understanding neurodevelopmental defects SMA crucial for optimizing therapeutic interventions. Using spinal cord cerebral organoids generated from multiple type I donors, we revealed widespread mechanisms beyond neuron degeneration. Single-cell transcriptomics uncovered pervasive alterations across neural populations,...
Significance It is generally believed that the outcome of many inductive interactions occurring during development largely dependent on responding tissue, source signals playing a relatively minor part. Here, we compare induction neural plate by node, and placodes head mesoderm, show both inducing tissues elicit similar initial response but they later diverge. We characterize common state variety methods its similarity to ES cells, suggesting these inductions may begin with “reprogramming”...
Few families of signaling factors have been implicated in the control development. Here, we identify neuropeptides nociceptin and somatostatin, a neurotransmitter neuroendocrine hormone, as class developmental signals both chick zebrafish. We show that from anterior mesendoderm are required for formation placode progenitors, with one being somatostatin. Somatostatin controls ectodermal expression nociceptin, peptides regulate Pax6 lens olfactory progenitors. Consequently, loss somatostatin...
The inner ear is a complex vertebrate sense organ, yet it arises from simple epithelium, the otic placode. Specification towards fate requires diverse signals and transcriptional inputs that act sequentially and/or in parallel. Using chick embryo, we uncover novel genes gene regulatory network underlying commitment reveal dynamic changes expression. Functional analysis of selected transcription factors reveals genetic hierarchy transition progenitor to committed precursor, integrating known...
During neural circuit assembly, extrinsic signals are integrated into changes in growth cone (GC) cytoskeleton underlying axon guidance decisions. Microtubules (MTs) were shown to play an instructive role GC steering. However, the numerous actors required for MT remodeling during navigation and their precise mode of action far from being deciphered. Using loss- gain-of-function analyses zebrafish development, we identify this study meiotic clade adenosine triphosphatase Fidgetin-like 1...
Abstract During development cell commitment is regulated by inductive signals that are tightly controlled in time and space. In response, cells activate specific programmes, but the transcriptional circuits maintain identity a changing signalling environment often poorly understood. Specification of inner ear progenitors initiated FGF signalling. Here, we establish genetic hierarchy downstream systematic analysis many factors combined with network inference approach. We show rapidly...
Vertebrate ear progenitors are induced by fibroblast growth factor signalling, however the molecular mechanisms leading to coordinate activation of downstream targets yet be discovered. The ear, like other sensory placodes, arises from pre-placodal region at border neural plate. Using a multiplex NanoString approach, we determined response these FGF signalling examining changes more than 200 transcripts that define otic and crest plate territories. This analysis identifies new direct...
Abstract Segmentation of the vertebrate hindbrain leads to formation rhombomeres, each with a distinct anteroposterior identity. Specialised boundary cells form at segment borders that act as source or regulator neuronal differentiation. In zebrafish, there is spatial patterning neurogenesis in which non-neurogenic zones bounderies and centres, part mediated by Fgf20 signaling. To further understand control neurogenesis, we have carried out single cell RNA sequencing zebrafish three...
ABSTRACT The fibroblast growth factor pathway is essential for inner ear induction in many vertebrates, however how it regulates the chromatin landscape to coordinate activation of otic genes remains unclear. Here we show that FGF exposure sensory progenitors leads rapid deposition active marks H3K27ac near hundreds FGF-responsive, otic-epibranchial progenitor (OEP) genes, while depleted vicinity non-otic genes. Genomic regions gain act as cis-regulatory elements controlling OEP gene...